CAJ | 학술논문

目的:研究针刺降压和褪黑素(Mel)在下丘脑前核(AHA)引起应激性高血压大鼠血压降低的作用是否与延髓头端腹外侧区(rVLM)氨基酸类递质释放的改变有关,并分析Mel降压机制与针刺降压机制之间的关系.方法:采用足底电击结合噪声的方法建立应激性高血压大鼠模型;针刺应用电针的方法,穴位取双侧足三里;采用中枢核团微量注射的方法给药,观察动脉血压的改变,同时应用脑内微透析的方法取样,并利用高效液相色谱-荧光检测的方法,分析透析液中氨基酸含量的改变.结果:大鼠在接受应激处理后,血压升高,rVLM内谷氨酸(Glu)释放量增加,应激性高血压大鼠在经针刺处理后,血压回降,rVLM内Glu释放量回减.微量注射Mel至应激性高血压大鼠的AHA能使血压降低,rVLM内Glu释放量也减少,而γ-氨基丁酸(GABA)和牛磺酸(Tau)释放量则增加.在rVLM微量注射GABAA受体拮抗剂荷包牡丹碱(bicuc)则可部分阻断Mel在AHA引起的降压效应.结论:针刺降压与rVLM内Glu释放量减少有关,而Mel在AHA引起的降压效应则与rVLM内Glu释放量减少,以及GABA和Tau释放量增加有关.针刺降压和Mel在AHA引起的降压机制都与rVLM内Glu释放量减少有关.
목적:연구침자강압화퇴흑소(Mel)재하구뇌전핵(AHA)인기응격성고혈압대서혈압강저적작용시부여연수두단복외측구(rVLM)안기산류체질석방적개변유관,병분석Mel강압궤제여침자강압궤제지간적관계.방법:채용족저전격결합조성적방법건립응격성고혈압대서모형;침자응용전침적방법,혈위취쌍측족삼리;채용중추핵단미량주사적방법급약,관찰동맥혈압적개변,동시응용뇌내미투석적방법취양,병이용고효액상색보-형광검측적방법,분석투석액중안기산함량적개변.결과:대서재접수응격처리후,혈압승고,rVLM내곡안산(Glu)석방량증가,응격성고혈압대서재경침자처리후,혈압회강,rVLM내Glu석방량회감.미량주사Mel지응격성고혈압대서적AHA능사혈압강저,rVLM내Glu석방량야감소,이γ-안기정산(GABA)화우광산(Tau)석방량칙증가.재rVLM미량주사GABAA수체길항제하포모단감(bicuc)칙가부분조단Mel재AHA인기적강압효응.결론:침자강압여rVLM내Glu석방량감소유관,이Mel재AHA인기적강압효응칙여rVLM내Glu석방량감소,이급GABA화Tau석방량증가유관.침자강압화Mel재AHA인기적강압궤제도여rVLM내Glu석방량감소유관.
To investigate whether changes of amino acid neurotransmitter releases in the rostral ventrolateral medulla (rVLM) were related to acupuncture and to the antihypertensive effect of melatonin (Mel) microinjected into the anterior hypothalamic area (AHA) of rats with stress-induced hypertension (SIH), as well as to compare and analyze the relationship between the both antihypertensive mechanisms of acupuncture and of Mel in the AHA. Methods: Animal model of SIHR was made by electric foot shocks combined with noises. Electroacupuncture (EA) was used and "Zusanli" acupoint of both side was selected. The technique of drug microinjection into the brain was used to observe the change of blood pressure (BP), and synchronously, brain microdialysis was performed for collecting dialysate samples, and then the concentration of amino acid neurotransmitters in the dialysate samples was determined by high performance liquid chromatography combined with fluorescent detection (HPLC-FD). Results: After the animal received stress treatment, the BP elevated, synchronously, the release of glutamate (Glu) in the rVLM increased, and when EA was performed, the elevated BP of the rats with SIH decreased,simultaneously, the release of Glu in the rVLM decreased also. After Mel was microinjected of into the AHA of the SIHR, the elevated BP attenuated, meanwhile, the release of Glu decreased,and those of γ-aminobutyric acid (GABA) and taurine (Tau) increased in the rVLM.Administration ofbicuculline, an antagonist of GABAA receptor, into the rVLM prior to microinjection of Mel into the AHA could partially block the depressor effect of Mel in the AHA. Conclusion: The decrease in the release of Glu in the rVLM contributes to the antihypertensive effect of both acupuncture and Mel in the AHA in the rats with SIH, and the increase in the release of GABA and Tau in the rVLM is also important to Mel in the AHA.