Real-time PCR技术与传统培养法检测肠道沙门菌和志贺菌的比较
Real-time PCR기술여전통배양법검측장도사문균화지하균적비교
Comparison between real-time PCR technique and conventional culture assay for the quantitative detection of Salmonella and Shigella in intestinal tract
  • 万方数据
    中国卫生检验杂志 중국위생검험잡지
    CHINESE JOURNAL OF HEALTH LABORATORY TECHNOLOGY
    2007年 8期 1458-1460 ,共3页

    褚国方%张月娟%李明珠%沈隽卿%倪争鸣%曹钟艺

    저국방%장월연%리명주%침준경%예쟁명%조종예

    Real-time PCR技术%传统培养法%沙门菌%志贺菌 Real-time PCR技術%傳統培養法%沙門菌%誌賀菌
    Real-time PCR기술%전통배양법%사문균%지하균
    目的:Real-time PCR技术与传统培养法检测肠道致病菌的比较.方法:根据沙门菌、志贺菌的基因保守区设计特异性引物和Taqman探针,建立Real-time PCR技术,检测样本中的沙门菌、志贺菌,与传统培养法检测作对比.结果:Real-time PCR技术显示出特异性强、灵敏度高、操作简便快捷等优点.结论:Real-time PCR技术相比传统培养法更适用于肠道致病菌的检测和筛选.
    목적:Real-time PCR기술여전통배양법검측장도치병균적비교.방법:근거사문균、지하균적기인보수구설계특이성인물화Taqman탐침,건립Real-time PCR기술,검측양본중적사문균、지하균,여전통배양법검측작대비.결과:Real-time PCR기술현시출특이성강、령민도고、조작간편쾌첩등우점.결론:Real-time PCR기술상비전통배양법경괄용우장도치병균적검측화사선.
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