CAJ | 학술논문

目的应用双重标记技术观察咬肌神经切断对支配咬肌的三叉神经运动神经元所含高亲和性神经营养物质受体--Trk受体,即TrkA、TrkB和TrkC表达的影响.方法切断大鼠咬肌神经7和14 d后,对脑切片进行免疫组织化学染色并观察荧光金(FG)标记的三叉神经运动核(Mo5)神经元表达的三种Trk受体.结果(1)荧光金标记神经元中TrkA免疫反应阳性神经元比例无显著性变化(P＞0.05);(2)神经切断后7和14 d均观察到TrkB表达上调(P＜0.05);(3)神经切断后14 d观察到TrkC表达下调(P＜0.05).结论咬肌神经切断后,三叉神经运动核神经元所含不同Trk受体的表达存在差异性调节.
목적응용쌍중표기기술관찰교기신경절단대지배교기적삼차신경운동신경원소함고친화성신경영양물질수체--Trk수체,즉TrkA、TrkB화TrkC표체적영향.방법절단대서교기신경7화14 d후,대뇌절편진행면역조직화학염색병관찰형광금(FG)표기적삼차신경운동핵(Mo5)신경원표체적삼충Trk수체.결과(1)형광금표기신경원중TrkA면역반응양성신경원비례무현저성변화(P＞0.05);(2)신경절단후7화14 d균관찰도TrkB표체상조(P＜0.05);(3)신경절단후14 d관찰도TrkC표체하조(P＜0.05).결론교기신경절단후,삼차신경운동핵신경원소함불동Trk수체적표체존재차이성조절.
Objective With double-labeling technique, the present study aims to observe the effects of nerve transection on the trigeminal motoneurons innervating masseter with regard to the expression of high-affinity neurotrophin receptors, viz. tropomyosin-related kinase (Trk) receptors: TrkA, TrkB and TrkC. Methods 7 and 14 d following masseteric nerve transection in the rat, brain sections were processed immunohistochemically for the detection of the three Trk isoforms on the motor trigeminal nucleus ( Mo5 ) neurons, which were retrogradely identified with FluoroGold (FG). Results The results showed ( 1 ) no significant change in the percentage of TrkA-immunoreactive (IR) neurons to the total FG-la-beled neurons ( P ＞ 0.05 ), (2) up-regulation of TrkB at both 7 and 14 d after axotomy ( P ＜ 0.05 ) and ( 3 ) down-regu-lation of TrkC 14 d following nerve transection (P ＜ 0.05). Conclusion The expression of the individual Trk receptors on the Mo5 neurons were differentially regulated post masseteric nerve transection.