癌症
癌癥
암증
CHINESE JOURNAL OF CANCER
2001年
3期
229-235
,共7页
宋怀东%彭永德%胡仁明%陈家伦%黄秋花%周隽%吴昕彦%张庆华%任双喜%付刚
宋懷東%彭永德%鬍仁明%陳傢倫%黃鞦花%週雋%吳昕彥%張慶華%任雙喜%付剛
송부동%팽영덕%호인명%진가륜%황추화%주준%오흔언%장경화%임쌍희%부강
垂体瘤%基因表达谱%生物信息学%半定量RT-PCR
垂體瘤%基因錶達譜%生物信息學%半定量RT-PCR
수체류%기인표체보%생물신식학%반정량RT-PCR
目的：探讨垂体瘤与垂体组织差异表达基因的大规模筛选及鉴定。方法：垂体无功能腺瘤及正常垂体组织cDNA文库构建，大规模表达序列标签(expressed sequence tags，ESTs)测序、表达谱差异的生物信息学处理及半定量逆转录-聚合酶链反应(reverse transcriptase polymerase chain reaction，RT-PCR)鉴定。结果：从无功能腺瘤和正常垂体组织中分别获得1253和7222个质量满意的ESTs；垂体瘤中已知基因的品种数200个，经组织表达谱差异比较的统计学软件处理，在这些已知基因中，差异的可靠性≥0.99者38个，≥0.95者130个；其中与细胞生长、分化相关的G2类基因共17个；6个经半定量RT-PCR证实4个基因的表达在垂体瘤中明显高于正常垂体。结论：与细胞生长、分化相关的G2类基因MEIS2、SMT3C、C1D、BUB3在垂体瘤中表达高于正常垂体，推测可能与垂体瘤的发生有关。
目的：探討垂體瘤與垂體組織差異錶達基因的大規模篩選及鑒定。方法：垂體無功能腺瘤及正常垂體組織cDNA文庫構建，大規模錶達序列標籤(expressed sequence tags，ESTs)測序、錶達譜差異的生物信息學處理及半定量逆轉錄-聚閤酶鏈反應(reverse transcriptase polymerase chain reaction，RT-PCR)鑒定。結果：從無功能腺瘤和正常垂體組織中分彆穫得1253和7222箇質量滿意的ESTs；垂體瘤中已知基因的品種數200箇，經組織錶達譜差異比較的統計學軟件處理，在這些已知基因中，差異的可靠性≥0.99者38箇，≥0.95者130箇；其中與細胞生長、分化相關的G2類基因共17箇；6箇經半定量RT-PCR證實4箇基因的錶達在垂體瘤中明顯高于正常垂體。結論：與細胞生長、分化相關的G2類基因MEIS2、SMT3C、C1D、BUB3在垂體瘤中錶達高于正常垂體，推測可能與垂體瘤的髮生有關。
목적：탐토수체류여수체조직차이표체기인적대규모사선급감정。방법：수체무공능선류급정상수체조직cDNA문고구건，대규모표체서렬표첨(expressed sequence tags，ESTs)측서、표체보차이적생물신식학처리급반정량역전록-취합매련반응(reverse transcriptase polymerase chain reaction，RT-PCR)감정。결과：종무공능선류화정상수체조직중분별획득1253화7222개질량만의적ESTs；수체류중이지기인적품충수200개，경조직표체보차이비교적통계학연건처리，재저사이지기인중，차이적가고성≥0.99자38개，≥0.95자130개；기중여세포생장、분화상관적G2류기인공17개；6개경반정량RT-PCR증실4개기인적표체재수체류중명현고우정상수체。결론：여세포생장、분화상관적G2류기인MEIS2、SMT3C、C1D、BUB3재수체류중표체고우정상수체，추측가능여수체류적발생유관。
Objective： The aim of this study was to investigate the method of large-scale screening for genes differentially expressed in nonfunctional pituitary tumor and normal pituitary. Methods： cDNA libraries of nonfunctional pituitary tumor (NFPT) and human normal pituitary were constructed. Large-scale sequencing, bioinformatics and semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) were used. Significant difference of known genes expression in 2 tissues were analyzed by statistical software offered by Audic S and Claverie JM (http： //igs-server.cnrs-mrs.fr/). Results： A total of 1253 and 7222 ESTs with good quality were obtained from the tissues of NFPT and normal pituitary, respectively. Two hundred known genes were found in NFPT. Among them, 38 genes creditability of significant difference expression in the 2 tissues were over 0.99, and 130 were over 0.95. Out of the 130 differentially expressed genes in the 2 tissues, 17 genes were associated with cell differentiation and development (G2). 6 differentially expressed G2 category genes were selected and the liability of the expression significant difference in the 2 tissues was confirmed by semi-quantitative RT-PCR. Out of them, 4 were confirmed differentially expressed in the 2 tissues. Conclusion： MEIS2, SMT3C, C1D, and BUB3 genes associated with cell differentiation and development (G2) were expressed more in nonfunctional pituitary tumor than in normal pituitary. They may play a role in the pathogenesis of nonfunctional pituitary carcinoma.
