CAJ | 학술논문

特异引物对(TOX1P/1F；TOX2P/2F)用于检测微囊藻毒素合成酶基因mcyB片段在38种水华蓝藻中的分布情况。结果显示，所有能产生微囊藻毒素的微囊藻都有特异扩增条带，非产毒株则没有。几种常规的毒性检测方法验证了PCR方法所获结果的准确性。本研究发展了以全细胞PCR法检测mcyB片断，说明全细胞PCR检测法适用于不同来源的蓝藻材料。结果证明以DNA为基础鉴别产毒和非产毒微囊藻及其他水华蓝藻的方法是可行和实用的。
특이인물대(TOX1P/1F；TOX2P/2F)용우검측미낭조독소합성매기인mcyB편단재38충수화람조중적분포정황。결과현시，소유능산생미낭조독소적미낭조도유특이확증조대，비산독주칙몰유。궤충상규적독성검측방법험증료PCR방법소획결과적준학성。본연구발전료이전세포PCR법검측mcyB편단，설명전세포PCR검측법괄용우불동래원적람조재료。결과증명이DNA위기출감별산독화비산독미낭조급기타수화람조적방법시가행화실용적。
The present study surveyed the distribution ofmicrocystin-producing gene——mcyB by PCR among 38 bloom-forming cyanobacterial strains from FACHB-Collection. The results demonstrated that special amplifications were being gotten only from 19 toxic Microcystis strains, whereas it was deficient in nontoxic ones got none. The PCR results showed highly agreement with the toxicity of every strain determined by HPLC, ELISA, and Bioassay respectively. Furthermore, the traditional PCR protocols were simplified by checking the cyanobacteria cells directly instead of the previously used extracted genomic DNA. The whole cells PCR we developed in this study could be successfully applied to cultured algae materials, water samples and lyophilized cyanobacterial cells. Thus, discrimination of toxic and nontoxic Microcystis strains by molecular biological technologies proves to be practicable and efficacious.