南京师大学报(自然科学版)
南京師大學報(自然科學版)
남경사대학보(자연과학판)
JOURNAL OF NANJING NORMAL UNIVERSITY(NATURAL SCIENCE)
2001年
1期
92-94
,共3页
孔亚慧%张锡然%曹祥荣%薛建丽
孔亞慧%張錫然%曹祥榮%薛建麗
공아혜%장석연%조상영%설건려
冻存%毛冠鹿%细胞株%细胞遗传学效应
凍存%毛冠鹿%細胞株%細胞遺傳學效應
동존%모관록%세포주%세포유전학효응
对液氮冻存19年的雄性毛冠鹿的胚肺细胞株进行复苏培养并对其核型进行观察,结果发现F21代时细胞株的核型已发生了变化,出现了一对大的末端着丝粒染色体.C带显示这一对染色体异染色质丰富,与已存在的相关染色体同源.这一核型不同于已报道的核型,是一种新的核型.
對液氮凍存19年的雄性毛冠鹿的胚肺細胞株進行複囌培養併對其覈型進行觀察,結果髮現F21代時細胞株的覈型已髮生瞭變化,齣現瞭一對大的末耑著絲粒染色體.C帶顯示這一對染色體異染色質豐富,與已存在的相關染色體同源.這一覈型不同于已報道的覈型,是一種新的覈型.
대액담동존19년적웅성모관록적배폐세포주진행복소배양병대기핵형진행관찰,결과발현F21대시세포주적핵형이발생료변화,출현료일대대적말단착사립염색체.C대현시저일대염색체이염색질봉부,여이존재적상관염색체동원.저일핵형불동우이보도적핵형,시일충신적핵형.
A lung cell strain of a male embryo of Elaphodus cephalophus wasresuscitated after being preserved in liquid nitrogen for about 19 years. Cytogenetics analysis showed that a new karyotype had been found at the 21st generation of cell strain. This new karyotype had more 48 chromosomes and a pair of big telocentric chromosomes compared to only single in the original karyotype of 48 chromosomes. The frequency of cells with such a pair of chromosomes greatly increased as the growth of cell. The results show the unstability of the cell strain of Elaphodus cephalophus.