CAJ | 학술논문

目的比较孕酮的还原性代谢产物四氢孕酮和地西泮抗焦虑作用.方法 C57小鼠腹腔注射四氢孕酮、地西泮或赋形剂,20 min后观察在架高十字迷宫试验中的表现,测定其自发活动.结果腹腔注射四氢孕酮0.1 mg.kg-1,明显缩短小鼠进入十字迷宫开臂的潜伏期[从(31.30±8.39)s减少到(8.80±6.00)s,P<0.001],并显著增加进入十字迷宫开臂的次数(从1.20± 0.42增到4.80±1.75,P<0.001)及在开臂的滞留时间占总时间的百分比(从7.13%增加到32.50%,P<0.001).而腹腔注射地西泮0.25 mg·kg-1的抗焦虑作用弱于四氢孕酮.自发活动实验显示,0.5 mg·kg-1地西泮明显减少小鼠自发活动(P<0.01),而0.1 mg·kg-1地西泮和0.25 mg·kg-1的四氢孕酮均不影响小鼠自发活动.结论试验结果提示神经甾体和地西泮对小鼠行为有不同的作用.四氢孕酮具选择性抗焦虑作用而不影响自发活动,可望成为地西泮抗焦虑的代用品.
목적비교잉동적환원성대사산물사경잉동화지서반항초필작용.방법 C57소서복강주사사경잉동、지서반혹부형제,20 min후관찰재가고십자미궁시험중적표현,측정기자발활동.결과복강주사사경잉동0.1 mg.kg-1,명현축단소서진입십자미궁개비적잠복기[종(31.30±8.39)s감소도(8.80±6.00)s,P<0.001],병현저증가진입십자미궁개비적차수(종1.20± 0.42증도4.80±1.75,P<0.001)급재개비적체류시간점총시간적백분비(종7.13%증가도32.50%,P<0.001).이복강주사지서반0.25 mg·kg-1적항초필작용약우사경잉동.자발활동실험현시,0.5 mg·kg-1지서반명현감소소서자발활동(P<0.01),이0.1 mg·kg-1지서반화0.25 mg·kg-1적사경잉동균불영향소서자발활동.결론시험결과제시신경치체화지서반대소서행위유불동적작용.사경잉동구선택성항초필작용이불영향자발활동,가망성위지서반항초필적대용품.
To compare the anxiolytic effects of reduced metabolite of progesterone and benzodiazepine.Methods　The effects of allopregnanolone and diazepam on spontaneous locomotor activity and on exploration in the elevated plus-maze were studied in C57 mice 20 min after vehicle or drug intraperitoneal administration.Results Allopregnanolone (0.1 mg.kg-1,ip) elicited marked anxiolytic effects in terms of significantly reducing the latency to enter the open arm from (31.30±8.39)s to (8.80±6.00)s,(P<0.001),and significantly increasing both the number of open arm entries from 1.20 ± 0.42 to 4.80 ±1.75,(P<0.001) and the proportion of total time spent on the open arm from 7.13％ to 32.50％,(P<0.001).Meanwhile,the diazepam (0.25 mg·kg-1) produced a lower anxiolytic effect comparing to that of the allopregnanolone.Analysis of spontaneous locomotor activity showed while 0.5 mg·kg-1 of diazepam decreased the locomotor activity (P<0.01),neither 0.1 mg·kg-1 of allopregnanolone nor 0.25 mg·kg-1 of diazepam affect the locomotor activity score.Conclusion Together,these results provide evidence for differential behavioral actions of the neurosteroids and benzodiazepines.Since the allopregnanolone produce a selective anxiolytic effect without affecting the spontaneous locomotor activity,the allopregnanolone may be a better alternative for diazepam in treating anxiety.