华人消化杂志
華人消化雜誌
화인소화잡지
WORLD CHINESE JOURNAL OF DIESTION
1998年
8期
650-652
,共3页
崔龙%曹广文%王元和%屠岳%孟荣贵%高军%仇小芳%吴宗娣%谢苏庆
崔龍%曹廣文%王元和%屠嶽%孟榮貴%高軍%仇小芳%吳宗娣%謝囌慶
최룡%조엄문%왕원화%도악%맹영귀%고군%구소방%오종제%사소경
colorectal carcinoma%cytosine deaminase gene%retroviral vector%carcinoembryonic antigen%transcriptional regulatory sequence
AIM In order to express suicide gene specifically in colorectal carcinoma cells, we constructed the recombinant retroviral vector promoted by transcriptional regulatory sequences of carcinoembryonic antigen (CEA) gene.METHODS A 1.3 kb EcoRⅠ/ BamHⅠ fragment of the CD gene was inserted into pUC118, and then the HindⅢ/ EcoRⅠ CD fragment was isolated. A 420 bp HindⅢ/ BamHⅠ fragment from pCEA 424/ 2CAT was released and subcloned into pUC118 to harvest HindⅢ/ EcoRⅠ CEA promoter. These two fragments were ligated with the linearized retroviral vector GINa using T4 DNA ligase.RESULTS Restriction enzyme SalⅠ was used to digest the recombinants to identify a positive clone A7 and a 1.7 kb band was observed on agarose electrophoresis gel. The recombinant retroviral vector, GICEACDNa was constructed.CONCLUSION Success in construction of GICEACDNa provides a basis for colorectal carcinoma-specific gene therapy.
