CAJ | 학술논문

用台中65(粳稻)/ARC10313(籼稻)的重组近交系(F10)构建了RFLP连锁图谱, 含113个分布均匀的标记. 作成的图谱覆盖全基因组, 全图总长1462.4 cM, 图中标记位置与所使用的参照图谱基本符合. 利用该重组自交家系材料与亲本台中65回交得到BF1家系, 用于对小穗不育和花粉不育的QTL分析, 检测出3个小穗不育和1个花粉不育QTL, 且有一个小穗不育位点和花粉不育位点重叠于第7条染色体的标记R1789处. 已知的恢复基因Rf4与该座位对应. 正在建立该座位的近等基因系以进行基因精确定位和克隆.
용태중65(갱도)/ARC10313(선도)적중조근교계(F10)구건료RFLP련쇄도보, 함113개분포균균적표기. 작성적도보복개전기인조, 전도총장1462.4 cM, 도중표기위치여소사용적삼조도보기본부합. 이용해중조자교가계재료여친본태중65회교득도BF1가계, 용우대소수불육화화분불육적QTL분석, 검측출3개소수불육화1개화분불육QTL, 차유일개소수불육위점화화분불육위점중첩우제7조염색체적표기R1789처. 이지적회복기인Rf4여해좌위대응. 정재건립해좌위적근등기인계이진행기인정학정위화극륭.
Quantitative trait locus (QTL) analysis has been carried out to identify genes conferring spikelet sterility and pollen sterility in rice. As a first step, an RFLP linkage map based on the recombinant inbred lines (RILs) was constructed. 125 RILs (F10) derived from a cross between a Japonica cultivar Taichung 65 and an Indica cultivar ARC10313 were developed. The RFLP map contained 113 well-dispersed RFLP markers. Total map length was 1462.4 cM Linkage arrangement of the RFLP markers was in good agreement with that of the previously constructed maps. 63 backcross F1 lines (BF1) derived from RILs were used as a segregating population for QTL analysis. Three QTLs for F1 spikelet sterility were detected on Chromosome 1, 7 and 11. Among them, a QTL near the RFLP marker R1789 (chromosome 7) was detected at significant level P=0.0001, and one QTL for F1 pollen sterility was also detected near R1789 marker. Rf4 on chromosome 7 is known as fertility restorer gene. Above QTLs detected on chromosome 7 in this study appeared to correspond to Rf4 locus.