中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2001年
6期
550-552
,共3页
缺氧%哮喘%内皮缩血管肽类%嗜酸细胞
缺氧%哮喘%內皮縮血管肽類%嗜痠細胞
결양%효천%내피축혈관태류%기산세포
目的：探讨模拟高原防治哮喘的机理。方法：用卵蛋白致敏和诱发复制哮喘动物模型，分为哮喘发作组、缓解组和低压舱治疗组，并设立正常对照组进行以下检测：①放免法测定血浆及支气管灌洗液(BALF)中内皮素(ET)含量及血浆皮质醇水平；②肺组织切片光镜检查。结果：①光镜下哮喘发作组肺组织切片见嗜酸性粒细胞（EOS）浸润明显，缓解组浸润较少，治疗组明显减少；②血浆皮质醇水平发作组、缓解组分别明显高于和低于正常组，治疗组与正常组无明显差异；血浆ET含量哮喘缓解组明显高于其他3组，而BALF中ET含量则为发作组明显高于其他3组，后3组之间无明显差异。结论：低压低氧治疗后，哮喘豚鼠血浆及BALF中内皮素降低而血浆中皮质醇增高，肺组织嗜酸性粒细胞浸润减少，这可能是模拟高原环境防治哮喘的机理之一。
目的：探討模擬高原防治哮喘的機理。方法：用卵蛋白緻敏和誘髮複製哮喘動物模型，分為哮喘髮作組、緩解組和低壓艙治療組，併設立正常對照組進行以下檢測：①放免法測定血漿及支氣管灌洗液(BALF)中內皮素(ET)含量及血漿皮質醇水平；②肺組織切片光鏡檢查。結果：①光鏡下哮喘髮作組肺組織切片見嗜痠性粒細胞（EOS）浸潤明顯，緩解組浸潤較少，治療組明顯減少；②血漿皮質醇水平髮作組、緩解組分彆明顯高于和低于正常組，治療組與正常組無明顯差異；血漿ET含量哮喘緩解組明顯高于其他3組，而BALF中ET含量則為髮作組明顯高于其他3組，後3組之間無明顯差異。結論：低壓低氧治療後，哮喘豚鼠血漿及BALF中內皮素降低而血漿中皮質醇增高，肺組織嗜痠性粒細胞浸潤減少，這可能是模擬高原環境防治哮喘的機理之一。
목적：탐토모의고원방치효천적궤리。방법：용란단백치민화유발복제효천동물모형，분위효천발작조、완해조화저압창치료조，병설립정상대조조진행이하검측：①방면법측정혈장급지기관관세액(BALF)중내피소(ET)함량급혈장피질순수평；②폐조직절편광경검사。결과：①광경하효천발작조폐조직절편견기산성립세포（EOS）침윤명현，완해조침윤교소，치료조명현감소；②혈장피질순수평발작조、완해조분별명현고우화저우정상조，치료조여정상조무명현차이；혈장ET함량효천완해조명현고우기타3조，이BALF중ET함량칙위발작조명현고우기타3조，후3조지간무명현차이。결론：저압저양치료후，효천돈서혈장급BALF중내피소강저이혈장중피질순증고，폐조직기산성립세포침윤감소，저가능시모의고원배경방치효천적궤리지일。
AIM: To explore the mechanism of the therapeutic effect of hypobaric hypoxia on allergic asthmas guinea pigs. METHODS：After the model of asthma was established with ovalbumin(OA) challenge in OA sensitized guinea pigs, the animals were randomized into the asthmas group(AG), the alleviative group(ALG) and hypobaric hypoxia treated group(HHTG). The levels of plasma cortisol and endothelin(ET), ET in bronchoalveolar lavage fluid(BALF) were determined by radioimmunoassay. The pulmonary pathological changes were observed with optical microscope. RESULTS:(1) Infiltration of eosinophils (EOS) in alveolar septum was found in AG and ALG, while it was reduced in HHTG. (2) The level of plasma cortisol was significantly higher in AG than in normal control group(NCG) (P＜0.01).But it was significantly lower in ALG than in NCG(P＜0.01), there was no difference in plasma cortisol level between HHTG and NCG. (3) The content of ET in plasma was significantly higher in ALG than that in NCG, AG and HHTG(P＜0.01), and ET level in BALF was significantly higher in AG than that in NCG, ALG and HHTG(P＜0.05),however, no significant difference was found among the latter three groups, respectively. CONCLUSION: After the treatment with hypobaric hypoxia, the ET levels of the plasma and BALF were decreaced and the content of plasma cortisol was increaced, and infiltration of EOS in alveolar septum was decreaced in asthmatic guinea pigs.That may be one of the mechanisms by which hypobavic hypoxia prevents and cures asthma.
