CAJ | 학술논문

观察了兵豆(Lens culinaris Medic.)初生根原皮层组织的细胞周期在其种子萌发过程中时间和空间上的动态变化.免疫组织化学和细胞学证据表明,原皮层细胞分别在种子吸胀大约13 h和17 h开始DNA复制和细胞分裂.最早进行DNA复制和细胞分裂的细胞位于远基端1 mm附近,但这些分裂细胞的DNA复制是在种子成熟过程中完成的,而不是在萌发后.第一个细胞周期的激活样式表明,这些细胞并不同步激活,而是依次进入细胞周期,且进入的次序与自身在根尖中的相对位置有关.在兵豆初生根原皮层组织中,邻近位置上的细胞的细胞周期同步化程度较高.
관찰료병두(Lens culinaris Medic.)초생근원피층조직적세포주기재기충자맹발과정중시간화공간상적동태변화.면역조직화학화세포학증거표명,원피층세포분별재충자흡창대약13 h화17 h개시DNA복제화세포분렬.최조진행DNA복제화세포분렬적세포위우원기단1 mm부근,단저사분렬세포적DNA복제시재충자성숙과정중완성적,이불시재맹발후.제일개세포주기적격활양식표명,저사세포병불동보격활,이시의차진입세포주기,차진입적차서여자신재근첨중적상대위치유관.재병두초생근원피층조직중,린근위치상적세포적세포주기동보화정도교고.
Cell cycle kinetic activity in the cortical cells of the lentil (Lens culinaris Medic. cv. Verte du Puy) primary root during germination was examined both temporally and spatially. Immunohistochemical and cytological evidence indicated that DNA replication and cell division started in the cortical cells of the lentil primary root after around 13 and 17 h of imbibition, respectively. The first cells in DNA synthesis and the first mitotic figures all appeared in the cortical cells about 1 mm from the root-cap junction, but these divided cells had synthesized their DNA during the maturity of seed instead of during germination. The kinetic pattern of activity of the first cell cycle showed that these cells were not activated synchronously, but re-entered the cell cycle in turn depending on their places in the root tip. However, the adjacent cells partially synchronously proceeded their cell cycle.