中国公共卫生
中國公共衛生
중국공공위생
CHINA PUBLIC HEALTH
2007年
2期
202-204
,共3页
曾垂焕%唐萌%熊丽林%王晓娜%夏婷
曾垂煥%唐萌%熊麗林%王曉娜%夏婷
증수환%당맹%웅려림%왕효나%하정
八氯二丙醚%中国仓鼠肺细胞%DNA合成%DNA荧光定量
八氯二丙醚%中國倉鼠肺細胞%DNA閤成%DNA熒光定量
팔록이병미%중국창서폐세포%DNA합성%DNA형광정량
目的 研究八氯二丙醚对体外培养中国仓鼠肺细胞(CHL)DNA合成影响.方法 采用DNA荧光定量测定方法,观察不同染毒浓度(0.250,0.187,0.125,0.062,0.046,0.031 μg/ml)对CHL细胞DNA合成影响.结果 八氯二丙醚浓度为0.062,0.046 μg/ml时DNA合成量与对照组比差异有统计学意义,表现为合成增加(P＜0.05),浓度为0.250,0.187,0.125μg/ml时,则表现为合成抑制(P＜0.05),且呈剂量-反应关系(P＜0.05);0.250 μg/ml浓度在脱离染毒后2,4,6,12 h内DNA合成量持续下降.结论 在低浓度时(0.062,0.046 μg/ml),八氯二丙醚可引起CHL细胞DNA合成能力增加,而在高浓度时(0.250,0.187,0.125 μg/ml)则表现为DNA合成抑制,且这种抑制由DNA损伤引起.
目的 研究八氯二丙醚對體外培養中國倉鼠肺細胞(CHL)DNA閤成影響.方法 採用DNA熒光定量測定方法,觀察不同染毒濃度(0.250,0.187,0.125,0.062,0.046,0.031 μg/ml)對CHL細胞DNA閤成影響.結果 八氯二丙醚濃度為0.062,0.046 μg/ml時DNA閤成量與對照組比差異有統計學意義,錶現為閤成增加(P＜0.05),濃度為0.250,0.187,0.125μg/ml時,則錶現為閤成抑製(P＜0.05),且呈劑量-反應關繫(P＜0.05);0.250 μg/ml濃度在脫離染毒後2,4,6,12 h內DNA閤成量持續下降.結論 在低濃度時(0.062,0.046 μg/ml),八氯二丙醚可引起CHL細胞DNA閤成能力增加,而在高濃度時(0.250,0.187,0.125 μg/ml)則錶現為DNA閤成抑製,且這種抑製由DNA損傷引起.
목적 연구팔록이병미대체외배양중국창서폐세포(CHL)DNA합성영향.방법 채용DNA형광정량측정방법,관찰불동염독농도(0.250,0.187,0.125,0.062,0.046,0.031 μg/ml)대CHL세포DNA합성영향.결과 팔록이병미농도위0.062,0.046 μg/ml시DNA합성량여대조조비차이유통계학의의,표현위합성증가(P＜0.05),농도위0.250,0.187,0.125μg/ml시,칙표현위합성억제(P＜0.05),차정제량-반응관계(P＜0.05);0.250 μg/ml농도재탈리염독후2,4,6,12 h내DNA합성량지속하강.결론 재저농도시(0.062,0.046 μg/ml),팔록이병미가인기CHL세포DNA합성능력증가,이재고농도시(0.250,0.187,0.125 μg/ml)칙표현위DNA합성억제,차저충억제유DNA손상인기.
