CAJ | 학술논문

目的以菲为模型,分离能够以菲作为惟一碳源和能源进行生长繁殖的微生物,研究菲的降解特性,为多环芳烃的生物降解机制和工业应用的研究奠定基础.方法从焦化污水池附近的土壤中,通过选择性富集培养,筛选、分离具有菲降解能力的茵株,通过16s rDNA序列分析鉴定其种属,并在不同的温度和pH条件下,测定菌株的生长及对菲的降解性能.结果得到两株较好的菲降解茵,初步鉴定其分别属于不动杆菌属和假单胞茵属.其最适生长温度均为37℃,最适降解温度分别为34℃和37℃,最适生长pH分别为7.0和6.5～7.0,最适降解pH为7.0和6.5,两天内菲的最大降解率分别达到80.4%和86.6%.结论分离得到的两株茵对菲都具有较好的降解能力,且假单胞茵略微优于不动杆菌,降解机制及降解策略、工程应用研究正在进行中.
목적 이비위모형,분리능구이비작위유일탄원화능원진행생장번식적미생물,연구비적강해특성,위다배방경적생물강해궤제화공업응용적연구전정기출.방법 종초화오수지부근적토양중,통과선택성부집배양,사선、분리구유비강해능력적인주,통과16s rDNA서렬분석감정기충속,병재불동적온도화pH조건하,측정균주적생장급대비적강해성능.결과 득도량주교호적비강해인,초보감정기분별속우불동간균속화가단포인속.기최괄생장온도균위37℃,최괄강해온도분별위34℃화37℃,최괄생장pH분별위7.0화6.5～7.0,최괄강해pH위7.0화6.5,량천내비적최대강해솔분별체도80.4%화86.6%.결론 분리득도적량주인대비도구유교호적강해능력,차가단포인략미우우불동간균,강해궤제급강해책략、공정응용연구정재진행중.
Aim Indigenous aromatic hydrocarbons degraders ( AHD) are the primary member for bioremediation of oil-contaminated and chemical-contaminated soils. Screening of polycyclic aromatic hydrocarbons ( PAHs) -degrading strains and identification of their characteristics are the bases of PAHs biodegradation. Methods Selective enrichment culture was used to screen phenanthrene-degrading microorganisms from coke-plant wastewater-contami-nated soils. 16S rDNA gene sequence analysis was used to determine species of isolated microorganisms. Conditions of growth and phenanthrene degradability were optimized. Results Two phenanthrene-degrading strains belonging to Acinetobacter sp and Pseudomonas sp respectively were isolated. For the strain belonging to Acinetobacter sp, 80. 4% of phenanthrene was degraded under the optimized conditions 37℃ , pH =7.0 for growth and 34℃, pH = 7. 0 for phenanthrene degradation. 86. 6% of phenanthrene was degraded by Pseudomonas sp strain on the conditions of 371, pH =6. 5～7.0 for growth and 371 , pH =6. 5 for phenanthrene degradation. Conclusion The two isolated strains could degrade phenanthrene efficiently but Pseudomonas sp better. Rhaminolipid, an important biosurfactant produced by Pseudomonas may probably facilitate phenanthrene degradation. The study of degradation mechanism of PAHs and biodegradation of PAHs on industry scale is in progress.