CAJ | 학술논문

目的探讨百草枯中毒致急性肺损伤中热休克蛋白70(HSP70)的表达.方法 72只健康Wistar大鼠随机平均分成3组,分别腹腔注射生理盐水(A组)、20 mg/kg百草枯(B组)和40 mg/kg百草枯(C组).分别于1,2,3,6,12和24 h留取标本,应用免疫组织化学染色、Western blot方法测定肺组织中HSP70的蛋白表达.结果 B组和C组大鼠肺组织发生急性肺损伤.B组大鼠2 h后HSP70表达与A组相比开始增强,6 h为表达高峰,12 h表达减少,24 h表达水平与A组相当.C组1 h后HSP70表达与A组相比明显增强,3 h达到高峰,24 h后与A组水平一致.结论百草枯诱导的急性肺损伤可引起支气管、细支气管和肺泡上皮细胞HSP70应激性表达,提示HSP70对肺损伤起保护作用.
목적 탐토백초고중독치급성폐손상중열휴극단백70(HSP70)적표체.방법 72지건강Wistar대서수궤평균분성3조,분별복강주사생리염수(A조)、20 mg/kg백초고(B조)화40 mg/kg백초고(C조).분별우1,2,3,6,12화24 h류취표본,응용면역조직화학염색、Western blot방법측정폐조직중HSP70적단백표체.결과 B조화C조대서폐조직발생급성폐손상.B조대서2 h후HSP70표체여A조상비개시증강,6 h위표체고봉,12 h표체감소,24 h표체수평여A조상당.C조1 h후HSP70표체여A조상비명현증강,3 h체도고봉,24 h후여A조수평일치.결론 백초고유도적급성폐손상가인기지기관、세지기관화폐포상피세포HSP70응격성표체,제시HSP70대폐손상기보호작용.
Objective To investigate the expression of heat shock protein-70(HSP70)in acute lung injury induced by paraquat poisoning.Methods A total of 72 Wistar rats were randomly divided into 3 groups(n=24 each group)and intraperitoneally injected with normal saline in group A,20 mg/kg paraquat in group B,and 40 mg/kg paraquat in group C.The lungs of rats were excised 1,3,6,12,and 24 hours after the injection,respectively.The expression of HSP70 in the lung tissue was detected by immunohistochemistry and Western blot.Results Paraquat induced acute lung injury in groups B and C.In group B,the expression of HSP70 increased at 2 hours,reached the peak at 6 hours,decreased at 12 hours,and returned to the baseline level at 24 hours.In group C,the expression of HSP70 increased at 1 hour,reached the peak at 3 hours,and returned to the baseline level at 24 hours.Conclusion Paraquat-induced acute lung injury may contribute to the enhanced expression of HSP70 in the bronchus,bronchiole,and alveolar epithelial cells,which indicates that HSP70 has a protective effect on lung injury.