中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2011年
5期
381-385
,共5页
肝癌%MAGI1%PTEN%侵袭
肝癌%MAGI1%PTEN%侵襲
간암%MAGI1%PTEN%침습
hepatocellular carcinoma%MAGI1%PTEN%invasion
目的:探讨MAGI1基因在肝癌侵袭转移中的作用及分子机制.方法:将MAGI1过表达载体,稳定转染HepG2细胞(HepG2MAGI1),应用划痕愈合实验和基质胶侵袭实验检测HepG2MAGI1 和对照组细胞(HepG2)在细胞运动侵袭能力上的差别.采用Western印迹方法检测MAGI1和PTEN的表达,并分析其相关性.结果:HepG2MAGI1 细胞的划痕愈合能力明显弱于HepG2 细胞[(90±10)% vs.(50±15)%,P＜0.05],HepG2MAGI1 细胞穿过基质胶的细胞数亦明显少于HepG2细胞(68±18 vs.150±30,P＜0.05).HepG2MAGI1 细胞中PTEN的蛋白表达水平明显高于HepG2细胞(1.40±0.32 vs.0.28±0.15,P＜0.05).相关分析显示MAGI1和PTEN存在高度正相关(r=0.913,P＜0.01).结论:MAGI1可能通过调节PTEN蛋白表达抑制肝癌的侵袭运动能力.
目的:探討MAGI1基因在肝癌侵襲轉移中的作用及分子機製.方法:將MAGI1過錶達載體,穩定轉染HepG2細胞(HepG2MAGI1),應用劃痕愈閤實驗和基質膠侵襲實驗檢測HepG2MAGI1 和對照組細胞(HepG2)在細胞運動侵襲能力上的差彆.採用Western印跡方法檢測MAGI1和PTEN的錶達,併分析其相關性.結果:HepG2MAGI1 細胞的劃痕愈閤能力明顯弱于HepG2 細胞[(90±10)% vs.(50±15)%,P＜0.05],HepG2MAGI1 細胞穿過基質膠的細胞數亦明顯少于HepG2細胞(68±18 vs.150±30,P＜0.05).HepG2MAGI1 細胞中PTEN的蛋白錶達水平明顯高于HepG2細胞(1.40±0.32 vs.0.28±0.15,P＜0.05).相關分析顯示MAGI1和PTEN存在高度正相關(r=0.913,P＜0.01).結論:MAGI1可能通過調節PTEN蛋白錶達抑製肝癌的侵襲運動能力.
목적:탐토MAGI1기인재간암침습전이중적작용급분자궤제.방법:장MAGI1과표체재체,은정전염HepG2세포(HepG2MAGI1),응용화흔유합실험화기질효침습실험검측HepG2MAGI1 화대조조세포(HepG2)재세포운동침습능력상적차별.채용Western인적방법검측MAGI1화PTEN적표체,병분석기상관성.결과:HepG2MAGI1 세포적화흔유합능력명현약우HepG2 세포[(90±10)% vs.(50±15)%,P＜0.05],HepG2MAGI1 세포천과기질효적세포수역명현소우HepG2세포(68±18 vs.150±30,P＜0.05).HepG2MAGI1 세포중PTEN적단백표체수평명현고우HepG2세포(1.40±0.32 vs.0.28±0.15,P＜0.05).상관분석현시MAGI1화PTEN존재고도정상관(r=0.913,P＜0.01).결론:MAGI1가능통과조절PTEN단백표체억제간암적침습운동능력.
Objective To explore the biological function and molecular mechanism of membrane associated guanylate kinase,WW and PDZ domain containing 1 (MAGI1) in hepatocellular carcinoma.Methods HepG2MAGI1 stable cell line was constructed by transfecting HepG2 cells with pcDNA3.1-MAGI1 plasmid.Wound healing and invasion assay were performed to compare the migration and invasion ability of HepG2MAGI1 and HepG2 cells.Furthermore,the expression of MAGI1 and phosphatase and tensin homolog deleted on chromosome ten (PTEN) was also examined by Western blot and the relationship was analyzed.Results The wound healing assay showed that the closure of HepG2MAGI1 cells was significantly slower than that of HepG2 cells [(90±10)% vs.(50±15)%,P＜0.05],and the invasion assay showed that the number of HepG2MAGI1 cells that passed through the matrigel was fewer than HepG2 cells (68±18 vs.150±30,P＜0.05).The protein expression level of PTEN was significantly elevated in HepG2MAGI1 cells compared with HepG2 cells (1.40±0.32 vs.0.28±0.15,P＜0.05).MAGI1 and PTEN protein expression levels were positively correlated (r=0.913,P＜0.01).Conclusion MAGI1 may inhibit the cancer cell migration and invasion of hepatocellular carcinoma via regulating PTEN.
