中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2009年
4期
197-200
,共4页
林希%朱坚胜%王赓歌%朱敏%陈华忠%赵海红
林希%硃堅勝%王賡歌%硃敏%陳華忠%趙海紅
림희%주견성%왕갱가%주민%진화충%조해홍
肝功能衰竭%Toll样受体%内毒素%肿瘤坏死因子-α%白细胞介素-6
肝功能衰竭%Toll樣受體%內毒素%腫瘤壞死因子-α%白細胞介素-6
간공능쇠갈%Toll양수체%내독소%종류배사인자-α%백세포개소-6
Liver failure%Toll-like receptors%Endotoxin%Tumor necrosis factor-α%Interleukin-6
目的 探讨肝衰竭患者外周血单核细胞(PBMC)Toll样受体2(TLR2)和TLR4表达的变化及其在肝衰竭发病机制中的作用.方法 采用RT-PCR法检测健康对照组(20名)、慢性乙型肝炎组(CHB,20例)、肝衰竭早期组(18例)和肝衰竭中晚期组(14例)患者PBMC中rrLR2 mRNA和TLR4 mRNA的表达.ELISA法检测患者血中内毒素、TNF-α和IL-6的水平.结果 与健康对照组比较,CHB组、肝衰竭早期组和中晚期组患者PBMC中的TLR2 mRNA、TLR4 mRNA,以及血中内毒素、TNF-α和IL-6水平有所上升,差异具有统计学意义(F值分别为32.997、37.476、23.951、57.265和38.403,P值均<0.01).TLR2 mRNA的表达在肝衰竭中晚期组中高于早期组,而TLR4 mRNA的表达则相反.在肝衰竭早期和中晚期,TLR2 mRNA的表达与TNF-α、IL-6均有明显相关性(rTNF为0.865和0.921,rIL-6为0.762和0.851,P值均<0.01),TLR4 mRNA在肝衰竭早期与TNF-α和IL-6具有相关性(r值分别为0.917和0.788,P值均<0.01).结论 TLR2和TLR4介导的炎性反应可能参与了肝衰竭的发病机制.
目的 探討肝衰竭患者外週血單覈細胞(PBMC)Toll樣受體2(TLR2)和TLR4錶達的變化及其在肝衰竭髮病機製中的作用.方法 採用RT-PCR法檢測健康對照組(20名)、慢性乙型肝炎組(CHB,20例)、肝衰竭早期組(18例)和肝衰竭中晚期組(14例)患者PBMC中rrLR2 mRNA和TLR4 mRNA的錶達.ELISA法檢測患者血中內毒素、TNF-α和IL-6的水平.結果 與健康對照組比較,CHB組、肝衰竭早期組和中晚期組患者PBMC中的TLR2 mRNA、TLR4 mRNA,以及血中內毒素、TNF-α和IL-6水平有所上升,差異具有統計學意義(F值分彆為32.997、37.476、23.951、57.265和38.403,P值均<0.01).TLR2 mRNA的錶達在肝衰竭中晚期組中高于早期組,而TLR4 mRNA的錶達則相反.在肝衰竭早期和中晚期,TLR2 mRNA的錶達與TNF-α、IL-6均有明顯相關性(rTNF為0.865和0.921,rIL-6為0.762和0.851,P值均<0.01),TLR4 mRNA在肝衰竭早期與TNF-α和IL-6具有相關性(r值分彆為0.917和0.788,P值均<0.01).結論 TLR2和TLR4介導的炎性反應可能參與瞭肝衰竭的髮病機製.
목적 탐토간쇠갈환자외주혈단핵세포(PBMC)Toll양수체2(TLR2)화TLR4표체적변화급기재간쇠갈발병궤제중적작용.방법 채용RT-PCR법검측건강대조조(20명)、만성을형간염조(CHB,20례)、간쇠갈조기조(18례)화간쇠갈중만기조(14례)환자PBMC중rrLR2 mRNA화TLR4 mRNA적표체.ELISA법검측환자혈중내독소、TNF-α화IL-6적수평.결과 여건강대조조비교,CHB조、간쇠갈조기조화중만기조환자PBMC중적TLR2 mRNA、TLR4 mRNA,이급혈중내독소、TNF-α화IL-6수평유소상승,차이구유통계학의의(F치분별위32.997、37.476、23.951、57.265화38.403,P치균<0.01).TLR2 mRNA적표체재간쇠갈중만기조중고우조기조,이TLR4 mRNA적표체칙상반.재간쇠갈조기화중만기,TLR2 mRNA적표체여TNF-α、IL-6균유명현상관성(rTNF위0.865화0.921,rIL-6위0.762화0.851,P치균<0.01),TLR4 mRNA재간쇠갈조기여TNF-α화IL-6구유상관성(r치분별위0.917화0.788,P치균<0.01).결론 TLR2화TLR4개도적염성반응가능삼여료간쇠갈적발병궤제.
Objective To investigate the expression of Toll-like receptor 2 and 4 (TLR2 and TLR4) in peripheral blood mononuclear cells (PBMCs) and the serum inflammatory cytokine levels in patients with liver failure. Methods The expressions of TLR2 mRNA and TLR4 mRNA in PBMCs were detected by RT-PCR in 20 healthy controls, 20 chronic hepatitis B (CHB) patients, 18 liver failure patients in early stage and 14 in intermediate-end stage. The serum contents of endotoxin, TNF-α and IL-6 were detected by ELISA. Results Compared with the healthy controls, the expression of TLR2 mRNA and TLR4 mRNA, and the contents of endotoxin, TNF-α and IL-6 increased in CHB patients and liver failure patients ( both early stage and intermediate-end stage) ( F = 32.997, 37.476, 23. 951,57. 265 and 38. 403, P < 0.01 ). TLR2 mRNA expression in liver failure patients in intermediate-end stage was higher than that in the early stage, but that for TLR4 mRNA was lower than that in early stage. The expressions of TLR2 mRNA and TLR4 mRNA in PBMCs were significantly correlated with the contents of TNF-α and IL-6 ( r = 0. 917 and 0. 788, P < 0. 01 ). Conclusion The inflammation reaction mediated by TLR2 and TLR4 might participate in the pathogenesis of liver failure.
