中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2012年
2期
102-106
,共5页
王梅%姜梅杰%李玉臣%于亮
王梅%薑梅傑%李玉臣%于亮
왕매%강매걸%리옥신%우량
大蒜素%美罗培南%鲍氏不动杆菌%抗坏血酸%耐药
大蒜素%美囉培南%鮑氏不動桿菌%抗壞血痠%耐藥
대산소%미라배남%포씨불동간균%항배혈산%내약
Allicin%Meropenem%Acinetobacter baumannii%Ascorbic acid%Drug resistance
目的 体外探讨维生素C对大蒜素与美罗培南联合对碳青霉烯类抗生素耐药鲍曼不动杆菌杀菌作用的影响.方法 采用EDTA-Na2纸片复合法和改良三维实验对6株多耐药鲍曼不动杆菌产生碳青霉烯酶进行鉴定.采用MH肉汤法,确定大蒜素、美罗培南的最小抑菌浓度(MIC)值,计算两药联用的联合抑菌分数(FIC).应用微孔板生物检测法检测不同浓度大蒜素、美罗培南及大蒜素+美罗培南、大蒜素+维生素C(0.25 g/L)的抑菌率.检测大蒜素(32、64、128 mg/L)、大蒜素(128 mg/L)+维生素C(0.25 g/L)作用后细菌谷胱甘肽、抗超氧阴离子自由基和总巯基的变化.结果 6株多耐药鲍曼不动杆菌均产生碳青霉烯酶.大蒜素对6株耐碳青霉烯抗生素鲍曼不动杆菌的MIC值均为512 mg/L、美罗培南为128 mg/L、大蒜素+美罗培南两种药物联用FIC值为0.25.不同浓度大蒜素+美罗培南联用比同浓度的两种药物单独使用时抑菌率明显提高.0.25 g/L维生素C与不同浓度大蒜素联用时的抑菌率较单独使用同浓度的大蒜素显著下降(均P＜0.05).各浓度大蒜素(32、64、128 mg/L)细菌谷胱甘肽、抗超氧阴离子自由基、总巯基水平均低于对照组(均P＜0.05),不同浓度的大蒜素作用后,细菌谷胱甘肽、抗超氧阴离子自由基、总巯基水平也随其浓度的升高而逐渐降低(均P＜0.05)；128 mg/L大蒜素作用后谷胱甘肽、抗超氧阴离子自由基、总巯基的含量分别为(4.52±0.71) mgGSH/gport、(115.21±4.24) U/L、(0.11±0.04) mmol/gprot,均低于128 mg/L大蒜素+0.25 g/L维生素C组的(5.64±0.40) mgGSH/gport、(145.84±2.30)U/L、(0.18±0.03) mmol/gprot(均P＜0.05).结论 大蒜素和美罗培南联用可显著提高对耐碳青霉烯类抗生素鲍曼不动杆菌抑菌杀菌作用.维生素C则可减低大蒜素通过氧化应激对耐碳青霉烯类抗生素鲍曼不动杆菌产生的抑制、杀伤作用.
目的 體外探討維生素C對大蒜素與美囉培南聯閤對碳青黴烯類抗生素耐藥鮑曼不動桿菌殺菌作用的影響.方法 採用EDTA-Na2紙片複閤法和改良三維實驗對6株多耐藥鮑曼不動桿菌產生碳青黴烯酶進行鑒定.採用MH肉湯法,確定大蒜素、美囉培南的最小抑菌濃度(MIC)值,計算兩藥聯用的聯閤抑菌分數(FIC).應用微孔闆生物檢測法檢測不同濃度大蒜素、美囉培南及大蒜素+美囉培南、大蒜素+維生素C(0.25 g/L)的抑菌率.檢測大蒜素(32、64、128 mg/L)、大蒜素(128 mg/L)+維生素C(0.25 g/L)作用後細菌穀胱甘肽、抗超氧陰離子自由基和總巰基的變化.結果 6株多耐藥鮑曼不動桿菌均產生碳青黴烯酶.大蒜素對6株耐碳青黴烯抗生素鮑曼不動桿菌的MIC值均為512 mg/L、美囉培南為128 mg/L、大蒜素+美囉培南兩種藥物聯用FIC值為0.25.不同濃度大蒜素+美囉培南聯用比同濃度的兩種藥物單獨使用時抑菌率明顯提高.0.25 g/L維生素C與不同濃度大蒜素聯用時的抑菌率較單獨使用同濃度的大蒜素顯著下降(均P＜0.05).各濃度大蒜素(32、64、128 mg/L)細菌穀胱甘肽、抗超氧陰離子自由基、總巰基水平均低于對照組(均P＜0.05),不同濃度的大蒜素作用後,細菌穀胱甘肽、抗超氧陰離子自由基、總巰基水平也隨其濃度的升高而逐漸降低(均P＜0.05)；128 mg/L大蒜素作用後穀胱甘肽、抗超氧陰離子自由基、總巰基的含量分彆為(4.52±0.71) mgGSH/gport、(115.21±4.24) U/L、(0.11±0.04) mmol/gprot,均低于128 mg/L大蒜素+0.25 g/L維生素C組的(5.64±0.40) mgGSH/gport、(145.84±2.30)U/L、(0.18±0.03) mmol/gprot(均P＜0.05).結論 大蒜素和美囉培南聯用可顯著提高對耐碳青黴烯類抗生素鮑曼不動桿菌抑菌殺菌作用.維生素C則可減低大蒜素通過氧化應激對耐碳青黴烯類抗生素鮑曼不動桿菌產生的抑製、殺傷作用.
목적 체외탐토유생소C대대산소여미라배남연합대탄청매희류항생소내약포만불동간균살균작용적영향.방법 채용EDTA-Na2지편복합법화개량삼유실험대6주다내약포만불동간균산생탄청매희매진행감정.채용MH육탕법,학정대산소、미라배남적최소억균농도(MIC)치,계산량약련용적연합억균분수(FIC).응용미공판생물검측법검측불동농도대산소、미라배남급대산소+미라배남、대산소+유생소C(0.25 g/L)적억균솔.검측대산소(32、64、128 mg/L)、대산소(128 mg/L)+유생소C(0.25 g/L)작용후세균곡광감태、항초양음리자자유기화총구기적변화.결과 6주다내약포만불동간균균산생탄청매희매.대산소대6주내탄청매희항생소포만불동간균적MIC치균위512 mg/L、미라배남위128 mg/L、대산소+미라배남량충약물련용FIC치위0.25.불동농도대산소+미라배남련용비동농도적량충약물단독사용시억균솔명현제고.0.25 g/L유생소C여불동농도대산소련용시적억균솔교단독사용동농도적대산소현저하강(균P＜0.05).각농도대산소(32、64、128 mg/L)세균곡광감태、항초양음리자자유기、총구기수평균저우대조조(균P＜0.05),불동농도적대산소작용후,세균곡광감태、항초양음리자자유기、총구기수평야수기농도적승고이축점강저(균P＜0.05)；128 mg/L대산소작용후곡광감태、항초양음리자자유기、총구기적함량분별위(4.52±0.71) mgGSH/gport、(115.21±4.24) U/L、(0.11±0.04) mmol/gprot,균저우128 mg/L대산소+0.25 g/L유생소C조적(5.64±0.40) mgGSH/gport、(145.84±2.30)U/L、(0.18±0.03) mmol/gprot(균P＜0.05).결론 대산소화미라배남련용가현저제고대내탄청매희류항생소포만불동간균억균살균작용.유생소C칙가감저대산소통과양화응격대내탄청매희류항생소포만불동간균산생적억제、살상작용.
Objective To investigate the impact of vitamin C on bactericidal effect of allicin combined with meropenem on carbapenem- resistant Acinetobacter baumannii in vitro.Method Six carbapenemase-producing multiple drug-resistant Acinetobacter baumannii strains were identified by EDTA-Na2 paper composite method and modified three-dimensional experimental approach.The minimal inhibitory concentration (MIC) of allicin and meropenem was determined for calculation of combination inhibition fraction (FIC) index for combined therapy using MH broth.The bacterial inhibitory rates of allicin,meropenem,allicin combined with meropenem and allicin combined with vitamin C (0.25 g/L) in various concentrations were detected via microplate bio-detection assay.After adding allicin (32,64 mg/L and 128mg/L respectively) and the combination of allicin and 0.25 g/L vitamin C,the variations in glutathione,antisuperoxide anion free radicals and total thiol group levels within the bacteria were measured.Results All the six carbapenem-resistant Acinetobacter baumannii strains were found to produce carbapenemase.These six strains yielded MIC of 512 mg/L for allicin and 128 mg/L for meropenem.Allicin combined with meropenem was associated with FIG of 0.25.Various concentrations of allicin combined with meropenem resulted in a considerably higher bacterial inhibitory rates than either drug alone.By contrast,0.25 g/Lvitamin C combined with various concentrations of allicin resulted in marked reduction of bacterial inhibitory rates (all P＜0.05) as compared with equal concentration of allicin alone.In addition,lower glutathione,antisuperoxide anion and total thiol group levels (all P＜0.05) were revealed in bacteria treated with various concentrations of allicin (32,64 mg/L and 128 mg/L) as compared with control group.Increased allicin concentration was associated with gradual decline in bacterial glutathione,anti-superoxide anion and total thiol group levels (all P＜0.05).The 128 mg/L allicin-treated bacteria exhibited lower levels of glutathione [(4.52±0.71 ) mgGSH/gport vs (5.64±0.40) mgGSP/gport],anti-superoxide anion radical [ (115.21±4.24)U/L vs (145.84±2.30) U/L] and total thiol group [ (0.11±0.04) mmol/gport vs (0.18±0.03) mmol/gport],as compared with the strains treated with 128 mg/L allicin combined with 0.25 g/L vitamin C (all P＜0.05).Conclusions Allicin combined with meropenem significantly increases bactericidal and inhibitory effects on carbapenem-resistant Acinetobacter baumannii.Vitamin C may decrease bactericidal and inhibitory effect of allicin on carbapenem-resistant Acinetobacter baumannii via suppressing oxidative stress.
