CAJ | 학술논문

目的验证转内皮抑素基因抑制高转移性黑色素瘤细胞的转移能力.方法将合胰岛素信号肽序列的pcDNA3.1-Endo真核表达载体转染小鼠黑色素瘤高转移细胞株B16,运用RT-PCR和Western-blot验证Endo的表达后,进行黑色素瘤细胞的粘附实验、体外侵袭和运动实验以及C57BL/6小鼠的肺转移试验.结果转内皮抑素基因能明显抑制黑色素瘤细胞的粘附、体外侵袭和运动以及肺转移能力,其中粘附抑制率36.4%,体外侵袭抑制率48.4%,细胞运动功能抑制率52.1%,肺转移抑制率67.3%.结论转内皮抑素基因能明显抑制黑色素瘤细胞的高转移能力.
목적 험증전내피억소기인억제고전이성흑색소류세포적전이능력.방법 장합이도소신호태서렬적pcDNA3.1-Endo진핵표체재체전염소서흑색소류고전이세포주B16,운용RT-PCR화Western-blot험증Endo적표체후,진행흑색소류세포적점부실험、체외침습화운동실험이급C57BL/6소서적폐전이시험.결과 전내피억소기인능명현억제흑색소류세포적점부、체외침습화운동이급폐전이능력,기중점부억제솔36.4%,체외침습억제솔48.4%,세포운동공능억제솔52.1%,폐전이억제솔67.3%.결론 전내피억소기인능명현억제흑색소류세포적고전이능력.
Objective To validate genetic suppression of metastastic capability of highly metastastic melanoma cells by endostatin transfection.Method pcDNA3.1-Endo eukaryotic expression vector contained insulin signal peptide sequence was transfected into highly metastatic mice melanoma cell strain B 16.The expression of endostain was detected by RT-PCR and Western blot experiment,melanoma cells were determined with adhere experiment,in vitro invasion and migration experiment and pulmonary metastasis experiment on C57BL/6 mice.Result Endostatin can obviously inhibit the capability of adherence,in vitro invasion and migration and pulmonary metastasis of melanoma cells.Among them,adhere inhibition ratio was 67.3%,in vitro invasion inhibition ratio was 48.4%,cell migration inhibition ratiowas 52.1%and pulmonary metastasis inhibition ratio was 67.3%.Conclusion Endostatin transfection can obviously inhibit the highly metastatie capability of melanoma cells.