中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
10期
878-880
,共3页
郑梅梅%贺燕%马艳%马学强%邢华医%杜怡峰
鄭梅梅%賀燕%馬豔%馬學彊%邢華醫%杜怡峰
정매매%하연%마염%마학강%형화의%두이봉
海风藤%阿尔茨海默病%β-淀粉样蛋白%行为学%学习记忆
海風籐%阿爾茨海默病%β-澱粉樣蛋白%行為學%學習記憶
해풍등%아이자해묵병%β-정분양단백%행위학%학습기억
Piper kadsura ohwi%Alzheimer's disease%Beta-amyloid%Learning and memory
目的 研究寡聚体Aβ1-42与纤丝状Aβ1-42对大鼠行为学不同程度的影响及应用海风藤干预后AD模型大鼠行为学是否发生变化.方法 采用微渗泵向侧脑室持续灌注寡聚体Aβ1-42或纤丝状Aβ1-42法制作动物模型.术后寡聚体Aβ1-42+海风藤组和纤丝状Aβ1-42+海风藤组大鼠每天腹腔注射10％海风藤提取物,寡聚体Aβ1-42+二甲基亚枫(dimethyl sulfoxide,DMSO)组和纤丝状Aβ1-42+DMSO组大鼠每天腹腔注射2.5％ DMSO,共5周.应用水迷宫实验观察大鼠的学习记忆功能变化.结果 (1)水迷宫实验结果显示,寡聚体Aβ1-42组的平均潜伏期为(89.40±7.20)s,长于纤丝状Aβ1-42组(65.00±10.89)s;纤丝状Aβ1-42+海风藤组潜伏期为(34.00±11.26)s,短于纤丝状Aβ1.+DMSO组(60.6 +6.95)s和纤丝状Aβ1-42组；寡聚体Aβ1-42+海风藤组的潜伏期(65.33±8.89)s,短于寡聚体Aβ1-42组和寡聚体Aβ1-42+DMSO组(85.60±6.02)s;寡聚体Aβ1-42组大鼠经过平台位置的次数(3.00±0.71)、在靶象限的时间比率(0.23±0.02)和路程比率(0.23 ±0.04)少于纤丝状Aβ1-42组(0.25±0.01,0.26±0.03,6.00±1.58);纤丝状Aβ1-42+海风藤组的此三项指标多于纤丝状Aβ1-42+DMSO组和纤丝状Aβ1-42组；寡聚体Aβ1-42+海风藤组的此三项指标多于寡聚体Aβ1-42组和寡聚体Aβ1-42+DMSO组.以上对比具有统计学意义(P＜0.05).结论 寡聚体Aβ1-42对大鼠行为学的影响较纤丝状Aβ1-42对大鼠行为学的影响大；海风藤干预能部分改善Aβ1-42组大鼠的学习记忆能力.
目的 研究寡聚體Aβ1-42與纖絲狀Aβ1-42對大鼠行為學不同程度的影響及應用海風籐榦預後AD模型大鼠行為學是否髮生變化.方法 採用微滲泵嚮側腦室持續灌註寡聚體Aβ1-42或纖絲狀Aβ1-42法製作動物模型.術後寡聚體Aβ1-42+海風籐組和纖絲狀Aβ1-42+海風籐組大鼠每天腹腔註射10％海風籐提取物,寡聚體Aβ1-42+二甲基亞楓(dimethyl sulfoxide,DMSO)組和纖絲狀Aβ1-42+DMSO組大鼠每天腹腔註射2.5％ DMSO,共5週.應用水迷宮實驗觀察大鼠的學習記憶功能變化.結果 (1)水迷宮實驗結果顯示,寡聚體Aβ1-42組的平均潛伏期為(89.40±7.20)s,長于纖絲狀Aβ1-42組(65.00±10.89)s;纖絲狀Aβ1-42+海風籐組潛伏期為(34.00±11.26)s,短于纖絲狀Aβ1.+DMSO組(60.6 +6.95)s和纖絲狀Aβ1-42組；寡聚體Aβ1-42+海風籐組的潛伏期(65.33±8.89)s,短于寡聚體Aβ1-42組和寡聚體Aβ1-42+DMSO組(85.60±6.02)s;寡聚體Aβ1-42組大鼠經過平檯位置的次數(3.00±0.71)、在靶象限的時間比率(0.23±0.02)和路程比率(0.23 ±0.04)少于纖絲狀Aβ1-42組(0.25±0.01,0.26±0.03,6.00±1.58);纖絲狀Aβ1-42+海風籐組的此三項指標多于纖絲狀Aβ1-42+DMSO組和纖絲狀Aβ1-42組；寡聚體Aβ1-42+海風籐組的此三項指標多于寡聚體Aβ1-42組和寡聚體Aβ1-42+DMSO組.以上對比具有統計學意義(P＜0.05).結論 寡聚體Aβ1-42對大鼠行為學的影響較纖絲狀Aβ1-42對大鼠行為學的影響大；海風籐榦預能部分改善Aβ1-42組大鼠的學習記憶能力.
목적 연구과취체Aβ1-42여섬사상Aβ1-42대대서행위학불동정도적영향급응용해풍등간예후AD모형대서행위학시부발생변화.방법 채용미삼빙향측뇌실지속관주과취체Aβ1-42혹섬사상Aβ1-42법제작동물모형.술후과취체Aβ1-42+해풍등조화섬사상Aβ1-42+해풍등조대서매천복강주사10％해풍등제취물,과취체Aβ1-42+이갑기아풍(dimethyl sulfoxide,DMSO)조화섬사상Aβ1-42+DMSO조대서매천복강주사2.5％ DMSO,공5주.응용수미궁실험관찰대서적학습기억공능변화.결과 (1)수미궁실험결과현시,과취체Aβ1-42조적평균잠복기위(89.40±7.20)s,장우섬사상Aβ1-42조(65.00±10.89)s;섬사상Aβ1-42+해풍등조잠복기위(34.00±11.26)s,단우섬사상Aβ1.+DMSO조(60.6 +6.95)s화섬사상Aβ1-42조；과취체Aβ1-42+해풍등조적잠복기(65.33±8.89)s,단우과취체Aβ1-42조화과취체Aβ1-42+DMSO조(85.60±6.02)s;과취체Aβ1-42조대서경과평태위치적차수(3.00±0.71)、재파상한적시간비솔(0.23±0.02)화로정비솔(0.23 ±0.04)소우섬사상Aβ1-42조(0.25±0.01,0.26±0.03,6.00±1.58);섬사상Aβ1-42+해풍등조적차삼항지표다우섬사상Aβ1-42+DMSO조화섬사상Aβ1-42조；과취체Aβ1-42+해풍등조적차삼항지표다우과취체Aβ1-42조화과취체Aβ1-42+DMSO조.이상대비구유통계학의의(P＜0.05).결론 과취체Aβ1-42대대서행위학적영향교섬사상Aβ1-42대대서행위학적영향대；해풍등간예능부분개선Aβ1-42조대서적학습기억능력.
Objectives To explore the impact of Aβ oligomer(Aβo) and fibrillar Aβ( Aβf) on the ethology of model rat and to explore whether or not piper kadsura ohwi(PKO) can ameliorate the ethological changes.Methods AD animal model was made by continuous intracerebroventricular infusion of Aβ through mini-osmotic pump.After surgery,rats of Aβo + PKO group and Aβf + PKO group received intraperitoneal injection of 10％PKO everyday,rats from Aβo + DMSO group and Aβf + DMSO group received intraperitoneal injection of 2.5％ DMSO.The treatment lasted 5 weeks.Morris water maze experiment began on the 31st day after surgery.Results ( 1 ) Acquisition trials:the escape latency was longer in Aβo group ( ( 89.40 ± 7.20 ) s ) than that in Aβf group ( (65.00 ± 10.89 ) s ) ; escape latency was shorter in Aβf + PKO group ( ( 34.00 ± 11.26 ) s) than that in Aβf group and Aβf + DMSO group( (60.6 ± 6.95 ) s) ; escape latency was shorter in Aβ3o + PKO group ( ( 65.33 ±8.89) s) than that in Aβo group and Aβo + DMSO group ( ( 85.60 ± 6.02) s).( 2 ) Robe trial:the times ( 3.00 ±0.71 ) and the proportion of time (0.23 ± 0.02 ) and path(0.23 ± 0.04) spent in the target quadrant in Aβo group was less than that in Aβf group(6.00 ± 1.58,0.25 ± 0.01,0.26 ± 0.03 ) ; the three parameters in Aβf + PKOgroup were more than those in Aβ3f group and Aβf+ DMSO group; the three parameters in Aβo + PKO group were more than those in Aβo group and Aβo + DMSO group.Conclusions Aβ oligomer had a more severe impact on the ethology of AD model rats than fibrillar Aβ did; piper kadsura ohwi may ameliorate the ethological changes of AD model rats.
