中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2011年
10期
901-904
,共4页
袁平%吴文汇%刘岽%张锐%荆志成
袁平%吳文彙%劉崠%張銳%荊誌成
원평%오문회%류동%장예%형지성
高血压,肺性%心脏导管插入术%血管阻力%心排血量
高血壓,肺性%心髒導管插入術%血管阻力%心排血量
고혈압,폐성%심장도관삽입술%혈관조력%심배혈량
Hypertension,pulmonary%Heart catheterization%Vascular resistance%Cardiac output
目的 建立一种简单、可重复性强的测定正常及肺动脉高压大鼠肺血管阻力的方法.方法 雄性Sprague-Dawley( 180 ～ 200 g)大鼠45只,随机分为3组:正常对照组、低剂量野百合碱组(50 mg/kg)和高剂量野百合碱组(60 mg/kg).给予大鼠颈背部皮下一次性注射野百合碱建立肺动脉高压大鼠模型.大鼠肺动脉压力采用自制改良的末端呈圆弧形的PE- 50导管行右心导管法测定.心输出量利用热稀释法原理检测.肺血管阻力由平均肺动脉压力除心输出量得出.结果 三组大鼠肺动脉压力、心输出量及肺血管阻力的检测总成功率均分别为98％、100％和96％,组间差异无统计学意义.注射野百合碱21 d后,低、高剂量野百合碱组大鼠的平均肺动脉压均显著高于对照组[(43.1±0.8)mm Hg(1 mm Hg=0.133 kPa)、(54.8±2.2) mm Hg比(17.4±1.0) mm Hg,P均＜0.001],且高剂量组明显高于低剂量组(P＜0.001).低、高剂量野百合碱组大鼠心输出量均明显低于对照组[(77.5±6.9) ml/min、(71.0+6.7)ml/min比(126.8±3.9) ml/min,P均＜0.001],低、高剂量组间差异无统计学意义.低、高剂量野百合碱组大鼠肺血管阻力均显著高于对照组[(0.56±0.06) mm Hg·min-1·ml-1、(0.76±0.08)mm Hg·min-1·ml-1比(0.13±0.01)mm Hg- min-1·ml-1,P均＜0.001],且高剂量组明显高于低剂量组(P=0.01).结论 采用此方法检测大鼠肺血管阻力准确、可靠、操作性强,具有推广价值.
目的 建立一種簡單、可重複性彊的測定正常及肺動脈高壓大鼠肺血管阻力的方法.方法 雄性Sprague-Dawley( 180 ～ 200 g)大鼠45隻,隨機分為3組:正常對照組、低劑量野百閤堿組(50 mg/kg)和高劑量野百閤堿組(60 mg/kg).給予大鼠頸揹部皮下一次性註射野百閤堿建立肺動脈高壓大鼠模型.大鼠肺動脈壓力採用自製改良的末耑呈圓弧形的PE- 50導管行右心導管法測定.心輸齣量利用熱稀釋法原理檢測.肺血管阻力由平均肺動脈壓力除心輸齣量得齣.結果 三組大鼠肺動脈壓力、心輸齣量及肺血管阻力的檢測總成功率均分彆為98％、100％和96％,組間差異無統計學意義.註射野百閤堿21 d後,低、高劑量野百閤堿組大鼠的平均肺動脈壓均顯著高于對照組[(43.1±0.8)mm Hg(1 mm Hg=0.133 kPa)、(54.8±2.2) mm Hg比(17.4±1.0) mm Hg,P均＜0.001],且高劑量組明顯高于低劑量組(P＜0.001).低、高劑量野百閤堿組大鼠心輸齣量均明顯低于對照組[(77.5±6.9) ml/min、(71.0+6.7)ml/min比(126.8±3.9) ml/min,P均＜0.001],低、高劑量組間差異無統計學意義.低、高劑量野百閤堿組大鼠肺血管阻力均顯著高于對照組[(0.56±0.06) mm Hg·min-1·ml-1、(0.76±0.08)mm Hg·min-1·ml-1比(0.13±0.01)mm Hg- min-1·ml-1,P均＜0.001],且高劑量組明顯高于低劑量組(P=0.01).結論 採用此方法檢測大鼠肺血管阻力準確、可靠、操作性彊,具有推廣價值.
목적 건립일충간단、가중복성강적측정정상급폐동맥고압대서폐혈관조력적방법.방법 웅성Sprague-Dawley( 180 ～ 200 g)대서45지,수궤분위3조:정상대조조、저제량야백합감조(50 mg/kg)화고제량야백합감조(60 mg/kg).급여대서경배부피하일차성주사야백합감건립폐동맥고압대서모형.대서폐동맥압력채용자제개량적말단정원호형적PE- 50도관행우심도관법측정.심수출량이용열희석법원리검측.폐혈관조력유평균폐동맥압력제심수출량득출.결과 삼조대서폐동맥압력、심수출량급폐혈관조력적검측총성공솔균분별위98％、100％화96％,조간차이무통계학의의.주사야백합감21 d후,저、고제량야백합감조대서적평균폐동맥압균현저고우대조조[(43.1±0.8)mm Hg(1 mm Hg=0.133 kPa)、(54.8±2.2) mm Hg비(17.4±1.0) mm Hg,P균＜0.001],차고제량조명현고우저제량조(P＜0.001).저、고제량야백합감조대서심수출량균명현저우대조조[(77.5±6.9) ml/min、(71.0+6.7)ml/min비(126.8±3.9) ml/min,P균＜0.001],저、고제량조간차이무통계학의의.저、고제량야백합감조대서폐혈관조력균현저고우대조조[(0.56±0.06) mm Hg·min-1·ml-1、(0.76±0.08)mm Hg·min-1·ml-1비(0.13±0.01)mm Hg- min-1·ml-1,P균＜0.001],차고제량조명현고우저제량조(P=0.01).결론 채용차방법검측대서폐혈관조력준학、가고、조작성강,구유추엄개치.
Objective To establish an easy and repeatable method for determination of pulmonary vascular resistance in normal and pulmonary arterial hypertension (PAH) rats.Methods Forty-five Sprague-Dawley rats were randomly assigned into three groups:control group,low dose monocrotaline (MCT) group (50 mg/kg) and high dose MCT group (60 mg/kg).Rats in PAH groups received single subcutaneous injection of MCT.We measured pulmonary artery pressure by right heart catheterization using an improved hand-made PE-50 catheter.Cardiac output was calculated through thermodilution method.Pulmonary vascular resistance equals the mean pulmonary artery pressure divided by cardiac output.Results The total percentages of success to detect pulmonary artery pressure,cardiac output and pulmonary vascular resistance were 98％,100％ and 96％ respectively in 3 groups.Twenty-one days after MCT injection,mean pulmonary artery pressure significantly increased in MCT group compared to control group [ (43.1 + 0.8 ),(54.8 +2.2) vs.(17.4 + 1.0) mm Hg( 1 mm Hg =0.133 kPa),P ＜0.001 ],and the mPAP was also significantly higher in high dose MCT group than in low dose MCT group (P ＜ 0.001 ).Cardiac output was significantly lower in PAH rats than in control rats [(77.5 ±6.9),(71.0 ±6.7) vs.(126.8 ±3.9) ml/min,P ＜0.001 ].Pulmonary vascular resistance was significantly increased in PAH rats compared with control rats [(0.56 +0.06),(0.76+0.08) vs.(0.13 ±:0.01) mm Hg · min-1 · ml-1,P＜0.001].There were significant differences in both MCT-treated groups (P =0.01 ).Conclusions Pulmonary vascular resistance in rats could be reliably detected using the improved hand-made PE-50 right heart catheter.
