CAJ | 학술논문

目的探讨脂质体的局部淋巴结靶向性和富集性.方法 18只兔分为3组,每组6只.在兔双侧第一趾蹼皮下注射99mTc-氟尿嘧啶脂质体(99mTc-FL)各18.5 MBq,然后在注射后不同时间(第1组3 h,第2组6 h,第3组8 h)检测局部淋巴结、非引流淋巴结、血、尿、肝、脾、肺、肾、心、肠等组织的放射性.结果3、6和8 h组的每克局部淋巴结摄取率分别为(2.32±0.75)%、(5.37±1.73)%和(8.61±1.89)%(P＜0.05);3 h组每克局部淋巴结与非引流淋巴结、血、尿、肝、脾、肺、肾、心、肠等组织摄取率的均数比分别为232.00、16.57、23.20、29.00、19.33、25.78、46.40、46.40和25.78,6 h组分别为89.50、41.31、18.52、67.13、41.31、25.57、134.25、59.67和59.67,8 h组分别为86.10、61.50、16.56、53.81、57.40、10.01、107.63、107.63和86.10,差异有统计学意义(P＜0.01).结论 FL可经淋巴途径缓慢流向局部淋巴结,并不直接进入血液循环,造成局部淋巴结靶向富集.脂质体的淋巴结靶向富集提示胸部恶性肿瘤局部淋巴靶向化疗的可行性.
목적 탐토지질체적국부림파결파향성화부집성.방법 18지토분위3조,매조6지.재토쌍측제일지복피하주사99mTc-불뇨밀정지질체(99mTc-FL)각18.5 MBq,연후재주사후불동시간(제1조3 h,제2조6 h,제3조8 h)검측국부림파결、비인류림파결、혈、뇨、간、비、폐、신、심、장등조직적방사성.결과3、6화8 h조적매극국부림파결섭취솔분별위(2.32±0.75)%、(5.37±1.73)%화(8.61±1.89)%(P＜0.05);3 h조매극국부림파결여비인류림파결、혈、뇨、간、비、폐、신、심、장등조직섭취솔적균수비분별위232.00、16.57、23.20、29.00、19.33、25.78、46.40、46.40화25.78,6 h조분별위89.50、41.31、18.52、67.13、41.31、25.57、134.25、59.67화59.67,8 h조분별위86.10、61.50、16.56、53.81、57.40、10.01、107.63、107.63화86.10,차이유통계학의의(P＜0.01).결론 FL가경림파도경완만류향국부림파결,병불직접진입혈액순배,조성국부림파결파향부집.지질체적림파결파향부집제시흉부악성종류국부림파파향화료적가행성.
Objective To investigate the feasibility of regional lymph nodes targetting with enrichment of radioactive 99mTc-polyphase liposome of 5-fluorouracil(99mTc-FL,FL).Methods 18 rabbits were randomly divided into 3 groups,6 rabbits per group.All rabbits were injected hypodermally with of 99mTc-FL in the right and left big toe webs.18.5 MBq each side.The post-injection interval was 3 h in group 1,6 h in group 2,and 8 h in group 3.The radioactivity was examined in the resected local lymph nodes,non-draining lymph nodes,liver,spleen,kidney,heart,lung,intestines,and in blood and urine.Results The radioactive isotope uptake percentage(%)was 2.32±0.75 in group 1,5.37±1.73 in group 2,8.61±1.89 in group 3.The radioactive isotope uptake percentage(%)per gram in local lymph nodes was significantly different between each two groups among the 3 groups(P＜0.05).The ratios of-x of regional lymph nodes/non-draining lymph nodes,regional lymph nodes/blood,regional lymph nodes/urine,regional lymph nodes/liver,regional lymph nodes/spleen,regional lymph nodes/kidney,regional lymph nodes/heart,regional lymph nodes/lung,regional lymph nodes/intestine in group 1 were 232.00,16.57,23.20,29.00,19.33,25.78,46.40,46.40 and 25.78,respectively.The ratios in group 2 were 89.50,41.31,18.52,67.13,41.31,25.57,134.25,59.67 and 59.67,respectively.The ratios in group 3 were 86.10,61.50,16.56,53.81,57.40,10.01,107.63,107.63 and 86.10,respectively.The differences of radioactive isotope uptake percentage were statistically significant(P＜0.01)between regional lymph nodes and other organs,i.e.non-draining lymph nodes,blood,urine,liver,spleen,kidney,heart,lung and ifftestine per gram in each group.Conclusion The radioactive 99m Tc-FL may slowly flow into regional lymphatic chains rather than directly enter blood circulation.So 99mTc-FL can be highly accumulated in the local lymph nodes.This regional lymph nodes targetting with enrichment of radioactive 99mTc-FL evidently indicates the feasibility of regional lymph system chemotherapy for pulmonary malignancies.