CAJ | 학술논문

目的研究当归补血汤(DBT)对骨髓移植小鼠免疫功能重建作用的影响.方法 BALB/c小鼠一次性接受全身137Csγ线致死量照射8.5Gy,照射后0-4h内经尾静脉输注同基因骨髓细胞107/只,同时给于不同剂量DBT,每日灌胃1次,连续15d.于骨髓移植后30、60d,检测以下指标:外周血红细胞(RBC)、白细胞(WBC)计数,骨髓有核细胞计数,胸腺、脾细胞总数和相应淋巴细胞亚类,以及反映免疫细胞功能的迟发型超敏反应(DTH)、空斑形成细胞数(PFC)等.结果经一定剂量DBT治疗的骨髓移植小鼠,其外周血中RBC、WBC计数,骨髓有核细胞计数,脾细胞和胸腺细胞总数,胸腺内各类细胞[双阴性细胞(DN)、双阳性细胞(DP)、单阳性细胞(SP)]的百分比值,与单纯骨髓移植小鼠比较差异有统计学意义(P<0.05),并且淋巴细胞的功能也得到进一步增强.到移植后60d,其各项指标进一步恢复.结论当归补血汤可以促进骨髓移植小鼠免疫功能的恢复.
목적 연구당귀보혈탕(DBT)대골수이식소서면역공능중건작용적영향.방법 BALB/c소서일차성접수전신137Csγ선치사량조사8.5Gy,조사후0-4h내경미정맥수주동기인골수세포107/지,동시급우불동제량DBT,매일관위1차,련속15d.우골수이식후30、60d,검측이하지표:외주혈홍세포(RBC)、백세포(WBC)계수,골수유핵세포계수,흉선、비세포총수화상응림파세포아류,이급반영면역세포공능적지발형초민반응(DTH)、공반형성세포수(PFC)등.결과 경일정제량DBT치료적골수이식소서,기외주혈중RBC、WBC계수,골수유핵세포계수,비세포화흉선세포총수,흉선내각류세포[쌍음성세포(DN)、쌍양성세포(DP)、단양성세포(SP)]적백분비치,여단순골수이식소서비교차이유통계학의의(P<0.05),병차림파세포적공능야득도진일보증강.도이식후60d,기각항지표진일보회복.결론 당귀보혈탕가이촉진골수이식소서면역공능적회복.
Objective To study the effect of Danggui Buxue Tang (DBT) on immunological reconstitution of bone marrow transplantation (BMT) in mice. Methods BALB/c mice were irradiated by 137Cs γ once 8.5 Gy, then the mice were engrafted with bone marrow cells (107 cells/mouse) within 4 hours lethal irradiation. And the mice were fed by DBT every day for 15 days. Flow cytometry technique combined with immunological methods were performed to evaluate immunological reconstitution of BMT mice in 30 and 60 days pest-transplantation. Peripheral blood RBC and WBC were counted, and nucleated cells were assayed in recipient bone marrow. Lymphocyte numbers in thymus and periphery were counted and subpopulations of the two origins were observed respectively. Lymphocyte proliferation induced by Con A and LPS, plaque-forming cell (PFC), delayed type hypersensitivity (DTH) were also examined in 30,60 days in post-transplantation respectively. Results Compared with BMT mice, BMT mice treated with a certain dose of DBT could increase the number of peripheral blood RBC and WBC in the recipients, and also could increase that of nucleated cells significantly. BMT mice treated with DBT could increase lymphocyte numbers in thymus and periphery, and improve thymocyte subpopulations, resulting in enhancement in immune function. Conclusion DBT can enhance the immunological reconstitution of BMT mice.