CAJ | 학술논문

本试验利用中性盐可以使蛋白质沉淀的原理,采用硫酸铵盐析法对鲶鱼粘液进行盐析,再进行离子交换层析采集免疫球蛋白溶液,然后通过冷冻干燥得到免疫球蛋白冻干样品,在低温下贮存备用,进行免疫球蛋白的抑菌活性的测定.抑菌实验采用滤纸片实验法,首先确定免疫球蛋白是否具有抑菌活性,其后改变免疫球蛋白样品溶液所处的外界环境.如温度、酸碱度等,通过定性实验,最终确定本试验所用免疫球蛋白的抑菌特性.试验结果为:从鲶鱼体表牯液中提取的免疫球蛋白具有抑菌活性,且对大肠杆菌抑菌效果最好.免疫球蛋白样品溶液在中性条件下,0-4℃抑菌活性较强;在低温贮存时温度控制在0-4℃,有助于生物活性的保存,但是随着时间的延长,生物活性也会逐渐减弱.
본시험이용중성염가이사단백질침정적원리,채용류산안염석법대염어점액진행염석,재진행리자교환층석채집면역구단백용액,연후통과냉동간조득도면역구단백동간양품,재저온하저존비용,진행면역구단백적억균활성적측정.억균실험채용려지편실험법,수선학정면역구단백시부구유억균활성,기후개변면역구단백양품용액소처적외계배경.여온도、산감도등,통과정성실험,최종학정본시험소용면역구단백적억균특성.시험결과위:종염어체표고액중제취적면역구단백구유억균활성,차대대장간균억균효과최호.면역구단백양품용액재중성조건하,0-4℃억균활성교강;재저온저존시온도공제재0-4℃,유조우생물활성적보존,단시수착시간적연장,생물활성야회축점감약.
This experiment take the principle of neutral salt can precipitate protein.Firstly salt out the mucus of catfish by ammonium sulfate,then extract immunoglobulin by ion exchange chromatography.Lastly dry the foregoing immunoglobulin by lyophilization and store in low temperature for evaluate the activity of immunoglobulin while inhibite bacteria.After that,changing the circumstance where the solution is in such as temprature and pH value and finally prove the speciality of the inhibition of immunoglobin.The result is:1)the immunoglobin from mucus of catfish has the activity to inhibite bacteria especially Escherichia coli.2)the activity is high in neutral circumstance and 0-4 ℃.3)The biological activity will descend when the time lasting.