中国医药生物技术
中國醫藥生物技術
중국의약생물기술
CHINESE MEDICINAL BIOTECHNOLOGY
2010年
1期
32-37
,共6页
汪爱媛%李永滨%许文静%彭江%郭全义%赵斌%张莉%卢世璧
汪愛媛%李永濱%許文靜%彭江%郭全義%趙斌%張莉%盧世璧
왕애원%리영빈%허문정%팽강%곽전의%조빈%장리%로세벽
合金%组织相容性%镍%离子%表面涂层
閤金%組織相容性%鎳%離子%錶麵塗層
합금%조직상용성%얼%리자%표면도층
Alloys%Histocompatibility%Nickel%Ions%Surface coating
目的 NiTi记忆合金表面分别进行不涂层、Ti涂层和TLNb涂层表面修饰后,对Ni~2+的释放安全性及生物相容性进行分析.方法 将NiTi记忆合金制备成10 mm×10 mm×10 mm立方体,一组采用等离子喷涂技术分别进行Ti涂层和TiNb涂层,另一组仅表面抛光清洗,不进行涂层.经X射线光电子能谱(XPS)测定植入体的表面成分,扫描电镜(SEM)和X射线荧光分析显微镜(XFAM)测定涂层厚度和形态.成年杂种犬18只,随机分为4组,NiTi合金组(无表面喷涂)、Ti涂层组、TiNb涂层组各5只,空白对照组3只.实验组分别于骶棘肌内埋入合金,每只犬每侧植入5枚,共10枚.空白对照组术式相同,只是不植入任何物体即缝合.12个月后处死取材,采用石墨炉原子吸收仪(GFAAS)分别检测心、肝、脾、肺、肾、脑、骨、鼻咽黏膜、血液、肌肉组织中Ni~2+>含量.对植入体周围纤维囊厚度进行显微镜下测量和结构分析,并进行统计学分析.结果 ①涂层表面光滑、平整、均匀,厚度约3.25 μm;②Ti和TiNb涂层可成功屏蔽合金表面Ni元素.NiTi合金组肝、肾组织中Ni~(2+)含量分别是空白组的1.6和2.4倍;Ti涂层组、TiNb涂层组、空白组之间Ni~(2+)含量无显著差异;③组织学观察三组植入体周围肌肉组织发现无明显巨噬细胞和炎性细胞浸润,具有良好的肌肉组织生物相容性;④植入体周围纤维囊厚度定量分析NiTi合金组、Ti涂层组和TiNb涂层组厚度分别为38.8、25.8和26.1 μm,NiTi组对Ti组和TiNb组差异极显著(P<0.01),而Ti组和TiNb组间无显著差异.结论 经Ti或TiNb涂层后的NiTi记忆合金可有效防止金属表面Ni~(2+)释放,提高NiTi合金的生物相容性和安全性.
目的 NiTi記憶閤金錶麵分彆進行不塗層、Ti塗層和TLNb塗層錶麵脩飾後,對Ni~2+的釋放安全性及生物相容性進行分析.方法 將NiTi記憶閤金製備成10 mm×10 mm×10 mm立方體,一組採用等離子噴塗技術分彆進行Ti塗層和TiNb塗層,另一組僅錶麵拋光清洗,不進行塗層.經X射線光電子能譜(XPS)測定植入體的錶麵成分,掃描電鏡(SEM)和X射線熒光分析顯微鏡(XFAM)測定塗層厚度和形態.成年雜種犬18隻,隨機分為4組,NiTi閤金組(無錶麵噴塗)、Ti塗層組、TiNb塗層組各5隻,空白對照組3隻.實驗組分彆于骶棘肌內埋入閤金,每隻犬每側植入5枚,共10枚.空白對照組術式相同,隻是不植入任何物體即縫閤.12箇月後處死取材,採用石墨爐原子吸收儀(GFAAS)分彆檢測心、肝、脾、肺、腎、腦、骨、鼻嚥黏膜、血液、肌肉組織中Ni~2+>含量.對植入體週圍纖維囊厚度進行顯微鏡下測量和結構分析,併進行統計學分析.結果 ①塗層錶麵光滑、平整、均勻,厚度約3.25 μm;②Ti和TiNb塗層可成功屏蔽閤金錶麵Ni元素.NiTi閤金組肝、腎組織中Ni~(2+)含量分彆是空白組的1.6和2.4倍;Ti塗層組、TiNb塗層組、空白組之間Ni~(2+)含量無顯著差異;③組織學觀察三組植入體週圍肌肉組織髮現無明顯巨噬細胞和炎性細胞浸潤,具有良好的肌肉組織生物相容性;④植入體週圍纖維囊厚度定量分析NiTi閤金組、Ti塗層組和TiNb塗層組厚度分彆為38.8、25.8和26.1 μm,NiTi組對Ti組和TiNb組差異極顯著(P<0.01),而Ti組和TiNb組間無顯著差異.結論 經Ti或TiNb塗層後的NiTi記憶閤金可有效防止金屬錶麵Ni~(2+)釋放,提高NiTi閤金的生物相容性和安全性.
목적 NiTi기억합금표면분별진행불도층、Ti도층화TLNb도층표면수식후,대Ni~2+적석방안전성급생물상용성진행분석.방법 장NiTi기억합금제비성10 mm×10 mm×10 mm립방체,일조채용등리자분도기술분별진행Ti도층화TiNb도층,령일조부표면포광청세,불진행도층.경X사선광전자능보(XPS)측정식입체적표면성분,소묘전경(SEM)화X사선형광분석현미경(XFAM)측정도층후도화형태.성년잡충견18지,수궤분위4조,NiTi합금조(무표면분도)、Ti도층조、TiNb도층조각5지,공백대조조3지.실험조분별우저극기내매입합금,매지견매측식입5매,공10매.공백대조조술식상동,지시불식입임하물체즉봉합.12개월후처사취재,채용석묵로원자흡수의(GFAAS)분별검측심、간、비、폐、신、뇌、골、비인점막、혈액、기육조직중Ni~2+>함량.대식입체주위섬유낭후도진행현미경하측량화결구분석,병진행통계학분석.결과 ①도층표면광활、평정、균균,후도약3.25 μm;②Ti화TiNb도층가성공병폐합금표면Ni원소.NiTi합금조간、신조직중Ni~(2+)함량분별시공백조적1.6화2.4배;Ti도층조、TiNb도층조、공백조지간Ni~(2+)함량무현저차이;③조직학관찰삼조식입체주위기육조직발현무명현거서세포화염성세포침윤,구유량호적기육조직생물상용성;④식입체주위섬유낭후도정량분석NiTi합금조、Ti도층조화TiNb도층조후도분별위38.8、25.8화26.1 μm,NiTi조대Ti조화TiNb조차이겁현저(P<0.01),이Ti조화TiNb조간무현저차이.결론 경Ti혹TiNb도층후적NiTi기억합금가유효방지금속표면Ni~(2+)석방,제고NiTi합금적생물상용성화안전성.
Objective To study the biocompatibility of Nitinol memory alloy with Ti and TiNb coated. And compared it with Nitinol alloy without coating. Methods NiTi memory alloys were made into 10 mm × 10 mm × 10 mm cubes. Their surfaces were coated with Ti (Ti group) and TLNb alloy (TiNb group) respectively by the Plasma Spray (PS) technique. While NiTi group were not treated on the surface. Surface component and coating thickness were measured before implanting. Eighteen mongrel dogs were divided into 4 groups, 5 dogs in each experimental group and 3 dogs in control group. The NiTi cubes, Ti coated cubes and TLNb coated cubes were implanted in the muscle by the sides of saerospinalis respectively in each experimental group. 10 pieces of each kind of implants were imbedded respectively in each dog. However the control group was imbedded nothing, The materials were obtained by sacrificing the dogs 12 months after the operation. Ni~(2+) concentration of heart, liver, spleen, lung, kidney, brain, nasopharyngeal mucous membrane, blood, bone tissue and muscular tissues around the implant were examined by Graphite Furnace Atomic Adsorption Spectrometry. The histological section was observed and the thickness and structure of the Fibrous Capsule Around Implants (FCAI) were measured quantitatively.
Results ①The coating was smooth and the thickness was approximately 3.25 μm. ② Two kinds of coating have succeeded in shielding of nickel element. In vivo, the Ni~2+ concentration in liver and kidney was much higher in NiTi group (P < 0.05) compared to Ti-coated and TiNb-coated and control groups. ③ The histologic observation did not have the obvious macrophage and the inflammation cell infiltrate in three groups. ④ The thickness of FCAI of the NiTi group, Ti-coated group and TiNb-coated group was 38.8, 25.8 and 26.1 μm respectively. The differences were remarkable between NiTi group and the other two groups (P < 0.01). But there was no obvious difference between Ti-coated group and TtNb-coated group. Condusion Ti-coated and TiNb-coated NiTi memory alloy could availably prevent the release of Ni~2+ and improve the biocompatibility and biosafety of NiTi alloys.
