CAJ | 학술논문

建立了麦冬中薯蓣皂甙元含量的高效毛细管电泳安培检测方法.着重探讨了缓冲溶液种类、浓度、酸碱度,工作电位,有机改性剂及其操作电压、进样时间等对检测的影响.Na_2B_4O_7-NaOH(Na_2B_4O_7浓度为30 mmol·L~(-1))为缓冲液,工作电位为0.4V,φ为7%甲醇为有机添加剂,进样时间为5 s,在15 kV分离高压,pH=9.5的碱性条件下柱端安培法检测了薯蓣皂甙元的含量.该法的线性范围为:100～1 mg·L~(-1),线性方程为Y=17 224 + 8 722c,相关系数r=0.999 5,检出限为0.1 mg·L~(-1).
건립료맥동중서여조대원함량적고효모세관전영안배검측방법.착중탐토료완충용액충류、농도、산감도,공작전위,유궤개성제급기조작전압、진양시간등대검측적영향.Na_2B_4O_7-NaOH(Na_2B_4O_7농도위30 mmol·L~(-1))위완충액,공작전위위0.4V,φ위7%갑순위유궤첨가제,진양시간위5 s,재15 kV분리고압,pH=9.5적감성조건하주단안배법검측료서여조대원적함량.해법적선성범위위:100～1 mg·L~(-1),선성방정위Y=17 224 + 8 722c,상관계수r=0.999 5,검출한위0.1 mg·L~(-1).
Diosgenin in radix ophiopogonis was determined by capillary electrophoresis with amperometric detection(CE-AD).The affected conditions,such as the running buffer pH value (pH),the types of running buffer,the concentration of running buffer,the detection potential,the applied voltage and the sampling time were investigated.The optimized condition was in a running buffer composed of 30 mmol/L Na_2B_4O_7 buffer at a pH 9.5,separation voltage at 15 kV,injection time for 5 s,organic additive for 7% methanol and detection potential at 0.4 V.A linear relation between peak area(Y) and mass concentration of diosgenin (c) was obtained in the range of 1～100mg·L~(-1) (r=0.999 5) and the determination limit is 0.1 mg·L~(-1).