生物技术
生物技術
생물기술
BIOTECHNOLOGY
2010年
2期
33-37
,共5页
单鼠李精糖脂%双鼠李糖糖脂%鼠李糖脂%电喷雾质谱%含量%离子化效率
單鼠李精糖脂%雙鼠李糖糖脂%鼠李糖脂%電噴霧質譜%含量%離子化效率
단서리정당지%쌍서리당당지%서리당지%전분무질보%함량%리자화효솔
single rhamnose glycolipid%double rhamnose glycolipid%rhamnolipid%ESI - MS%content%ionization efficiency
目的:确定微生物培养液中鼠李糖脂的组成、含量和鼠李糖脂在电喷雾质谱(ESI-MS)中的离子化效率.方法:用薄层色谱分离并结合ESI-MS分析培养液中的糖脂产物、测定样品中单鼠李糖脂和双鼠李糖脂比例及离子化强度.结果:根据苯酚-硫酸法和蒽酮光度法中糖及糖脂与吸光度的定量关系[A=0.0103X+0.0465(X为鼠李糖量,μg),A=0.0043X+0.0446(X为鼠李糖脂量,μg)],用化学计量方法确定了糖脂含量84.8%,其中单鼠李糖脂的质量分数0.344,双鼠李糖脂的质量分数0.656,并基于电喷雾质谱中的离子强度和测定的浓度计算了鼠李糖脂的离子化效率,双鼠李糖脂的钠离子化效率仅为单鼠李糖脂钠离子化效率的50%.结论:可用于定量分析单双糖脂及评价鼠李糖脂的生产.
目的:確定微生物培養液中鼠李糖脂的組成、含量和鼠李糖脂在電噴霧質譜(ESI-MS)中的離子化效率.方法:用薄層色譜分離併結閤ESI-MS分析培養液中的糖脂產物、測定樣品中單鼠李糖脂和雙鼠李糖脂比例及離子化彊度.結果:根據苯酚-硫痠法和蒽酮光度法中糖及糖脂與吸光度的定量關繫[A=0.0103X+0.0465(X為鼠李糖量,μg),A=0.0043X+0.0446(X為鼠李糖脂量,μg)],用化學計量方法確定瞭糖脂含量84.8%,其中單鼠李糖脂的質量分數0.344,雙鼠李糖脂的質量分數0.656,併基于電噴霧質譜中的離子彊度和測定的濃度計算瞭鼠李糖脂的離子化效率,雙鼠李糖脂的鈉離子化效率僅為單鼠李糖脂鈉離子化效率的50%.結論:可用于定量分析單雙糖脂及評價鼠李糖脂的生產.
목적:학정미생물배양액중서리당지적조성、함량화서리당지재전분무질보(ESI-MS)중적리자화효솔.방법:용박층색보분리병결합ESI-MS분석배양액중적당지산물、측정양품중단서리당지화쌍서리당지비례급리자화강도.결과:근거분분-류산법화은동광도법중당급당지여흡광도적정량관계[A=0.0103X+0.0465(X위서리당량,μg),A=0.0043X+0.0446(X위서리당지량,μg)],용화학계량방법학정료당지함량84.8%,기중단서리당지적질량분수0.344,쌍서리당지적질량분수0.656,병기우전분무질보중적리자강도화측정적농도계산료서리당지적리자화효솔,쌍서리당지적납리자화효솔부위단서리당지납리자화효솔적50%.결론:가용우정량분석단쌍당지급평개서리당지적생산.
Objective: To determine the composition and contents of the rhamnohpids in microbial culture meadia, and the ionization effi-ciency of rharmnolipids in Electro Spray Ionization Mass Spectrometry (ESI - MS).Method : Thin Layer Chromatography (TLC) combined with ESI - MS were used to analysize the composition of rhamnolipids, the ratio of single rhanmose glycolipid and double rhamnose glyco-lipid, and ionization strength.Result: Based on the relationship of rhamnose and glycolipid with absorbance in the methods of phenol -vitriol and anthrone photometry [A = 0.0103X + 0.0465 (X, rhamnose quantity, μg), A = 0.0043X + 0.0446 (X, thamnolipid quantity, μg)], the glycohpid content was deternined by thermometry as 84.8% with single rhamnose glyeolipid mass fraction of 0.344 and double rhamnose glycolipid mass fraction of 0.656.The ionization efficiency was calculated using intensity of eationized components in ESI - MS and concentration.The ionization efficiency of double rhamnose glycolipid in ESI - MS was low as 50% that of single rhamnose glycolipid.Conclusion: The results and procedure are useful for determining the ratio and content of glycolipids and evaluating the production of rh-amnolipids.
