CAJ | 학술논문

偏分离是指观察到的基因型频率偏离预期的孟德尔频率的遗传分离方式,在大多数的遗传定位研究中非常普遍.在之前我们发表的遗传连锁图中,107个SSR标记在BC1作图群体[(Emian 22×3-79)×Emian 22]中表现偏分离.为阐明这些偏分离标记的遗传机制及其在其它群体中的偏分离情况,将其中97个共显性标记在另外两个回交群体中进行验证.结果表明,原图谱中的61个偏分离标记在另外2个回交群体中都表现正常分离,说明杂交方式是导致偏分离的一个重要因素.36个偏分离标记至少在两个群体中仍表现偏分离,偏分离应该是配子选择的结果.偏分离标记分布于14条染色体上,其中D亚基因组上的分布多于A亚基因组.偏分离标记在在第2、第16和第18染色体上分布最多,暗示在这些染色体上存在偏分离位点,该结果有助于在棉花中鉴定偏分离位点.
편분리시지관찰도적기인형빈솔편리예기적맹덕이빈솔적유전분리방식,재대다수적유전정위연구중비상보편.재지전아문발표적유전련쇄도중,107개SSR표기재BC1작도군체[(Emian 22×3-79)×Emian 22]중표현편분리.위천명저사편분리표기적유전궤제급기재기타군체중적편분리정황,장기중97개공현성표기재령외량개회교군체중진행험증.결과표명,원도보중적61개편분리표기재령외2개회교군체중도표현정상분리,설명잡교방식시도치편분리적일개중요인소.36개편분리표기지소재량개군체중잉표현편분리,편분리응해시배자선택적결과.편분리표기분포우14조염색체상,기중D아기인조상적분포다우A아기인조.편분리표기재재제2、제16화제18염색체상분포최다,암시재저사염색체상존재편분리위점,해결과유조우재면화중감정편분리위점.
Segregation distortion(SD)is the deviation of genetic segregation ratios from their expected Mendelian fashion and is a common phenomenon found in most genetic mapping studies.In our previously genetic map published,107 SSR markers showed SD in the BC1 mapping population[(Emian 22×3-79)× Emian 221.To verify their segregation in other populations and to clarify the possible mechanism of SD,97 co-dominant markers of them were evaluated in another two backcross populations.The results showed that 61 distorted markers in the mapping population segregated normally in the other backcross populations,which implied that the cross way is a major factor affecting segregation distortion.Thirty-six markers showed segregation distortion in at least two populations,which is assumed to be caused by gametic selection.These distorted markers distributed on 14 chromosomes with more markers in D sub-genome than in A sub-genome.Chr.2,Chr.16,and Chr.18 were the chromosomes with more distorted markers than others,which implies that there must be segregation distortion loci on these chromosomes,leading us to identify segregation distortion loci in cotton.