CAJ | 학술논문

目的观察新西兰大耳白兔失血性休克后,肾组织中ATP酶和琥珀酸脱氢酶(SDH)活性变化,同时了解异丙酚预处理后,对以上各指标的影响,探讨异丙酚对失血性休克肾功能代谢的保护作用及其作用的可能机制.方法建立失血性休克动物模型,选健康新西兰大耳白兔32只,雌雄不拘,随机分为对照组、休克组、灌注组、异丙酚预处理组,每组8只.检测肾组织ATP酶和琥珀酸脱氢酶(SDH)活性变化.结果肾组织SDH和ATP酶活性,休克组和灌注组降低,分别与对照组相比差异有统计学意义(P＜0.01).异丙酚预处理组增高,与休克组相比差异也有统计学意义(P＜0.01).结论失血性休克可引起肾组织明显功能代谢障碍,异丙酚预处理后各项测定指标的活性均发生了变化,提示异丙酚对失血性休克肾代谢具有一定的保护作用,其保护作用是通过抗脂质过氧化、稳定细胞膜、提高生物氧化酶活性而起作用的.为临床应用提供了依据.
목적 관찰신서란대이백토실혈성휴극후,신조직중ATP매화호박산탈경매(SDH)활성변화,동시료해이병분예처리후,대이상각지표적영향,탐토이병분대실혈성휴극신공능대사적보호작용급기작용적가능궤제.방법 건립실혈성휴극동물모형,선건강신서란대이백토32지,자웅불구,수궤분위대조조、휴극조、관주조、이병분예처리조,매조8지.검측신조직ATP매화호박산탈경매(SDH)활성변화.결과 신조직SDH화ATP매활성,휴극조화관주조강저,분별여대조조상비차이유통계학의의(P＜0.01).이병분예처리조증고,여휴극조상비차이야유통계학의의(P＜0.01).결론 실혈성휴극가인기신조직명현공능대사장애,이병분예처리후각항측정지표적활성균발생료변화,제시이병분대실혈성휴극신대사구유일정적보호작용,기보호작용시통과항지질과양화、은정세포막、제고생물양화매활성이기작용적.위림상응용제공료의거.
Objective The change of kidney' sATPage and suecinate dehydrogqenage (SDH) in the New Zealan Rabbits after hemorrhagic shock;To observe the propofol pre-treatment effects;Discuss the protection of propofol to hemorrhagic shock's nephridium and the mechanism.Methods A model of hemorrhagic shock New Zealan Rabbit wag established by imitation.32 healthy New Zealan Rabbits were randomly divided into 4 groups ag following (n=8):the control group(CTG),shock group(Ⅰ group),perfusion group(Ⅱ group) and propofol pre-treatment group(Ⅲ group).Examine ATPase and succinate dehydrogqenage(SDH) in the kidney.Results SDH and ATPage in the kidney were decreased significantly in Ⅰ and Ⅱ group compared with CTG (P＜0.05～0.01),compared with Ⅰ group it increased significantly in Ⅲ group(P＜0.05～0.01).Conclusion Hemorrhagic shock can bring on a significant disfunction of metabolism.From the change of observed target after propofol pre-treatment we can conclude that propofol can take gome act of protection metabolism of kidhey by anti-lipoxidized、stabilized membrane of cell and elevated the activated of oxidage.