CAJ | 학술논문

目的探讨ghrelin基因Arg51Gln和Leu72Met多态性与甘肃东乡族、回族、汉族2型糖尿病及脂代谢的相关性.方法选取2008年10月至2010年2月在甘肃省人民医院、甘肃临夏市人民医院和东乡县医院初次就诊的2型糖尿病患者744例作为病例组(男386例,女358例),其中东乡族242例、回族242例、汉族260例.对照组673例为同期上述医院体检的健康志愿者,其中东乡族223例、回族203例、汉族247例.采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)检测ghrelin的Arg51Gln、Leu72Met基因型,并进行血糖、血脂等各项临床指标测定.基因型、等位基因频率分布比较用x2检验,正态分布的计量资料采用t检验,基因型与2型糖尿病和血脂的相关性使用简单相关分析和logistic回归分析.结果 (1)Arg51 Gln携带等位基因A(GA +AA)的个体较GG基因型个体患糖尿病的风险有增加趋势,Leu72Met等位基因A携带者(CA +AA)的个体较CC基因型个体患糖尿病的风险亦有增加趋势,但差异无统计学意义(均OR＞1,均P＞0.05).校正性别、年龄、体质指数、血压等因素后,仍未发现此两多态性位点与2型糖尿病的相关性(均P＞0.05).(2)校正性别、年龄、体质指数、总胆同醇等因素后,2型糖尿病组东乡族和回族Arg51Gln GA+ AA基因型与低密度脂蛋白胆固醇(LDL-C)水平呈正相关(r=0.406、0.377,均P＜0.05).而校正性别、年龄、体质指数、LDL-C后,2型糖尿病组东乡族和回族Leu72Met CA+ AA基因型与总胆固醇水平呈正相关(r=0.189、0.208,P＜0.05).结论 ghrelin基因、Arg51 Gln、Leu72Met多态性与东乡族、回族、汉族2型糖尿病可能无关,但与东乡族、回族血脂代谢可能有关.
목적 탐토ghrelin기인Arg51Gln화Leu72Met다태성여감숙동향족、회족、한족2형당뇨병급지대사적상관성.방법 선취2008년10월지2010년2월재감숙성인민의원、감숙림하시인민의원화동향현의원초차취진적2형당뇨병환자744례작위병례조(남386례,녀358례),기중동향족242례、회족242례、한족260례.대조조673례위동기상술의원체검적건강지원자,기중동향족223례、회족203례、한족247례.채용취합매련식반응-한제성편단장도다태성(PCR-RFLP)검측ghrelin적Arg51Gln、Leu72Met기인형,병진행혈당、혈지등각항림상지표측정.기인형、등위기인빈솔분포비교용x2검험,정태분포적계량자료채용t검험,기인형여2형당뇨병화혈지적상관성사용간단상관분석화logistic회귀분석.결과 (1)Arg51 Gln휴대등위기인A(GA +AA)적개체교GG기인형개체환당뇨병적풍험유증가추세,Leu72Met등위기인A휴대자(CA +AA)적개체교CC기인형개체환당뇨병적풍험역유증가추세,단차이무통계학의의(균OR＞1,균P＞0.05).교정성별、년령、체질지수、혈압등인소후,잉미발현차량다태성위점여2형당뇨병적상관성(균P＞0.05).(2)교정성별、년령、체질지수、총담동순등인소후,2형당뇨병조동향족화회족Arg51Gln GA+ AA기인형여저밀도지단백담고순(LDL-C)수평정정상관(r=0.406、0.377,균P＜0.05).이교정성별、년령、체질지수、LDL-C후,2형당뇨병조동향족화회족Leu72Met CA+ AA기인형여총담고순수평정정상관(r=0.189、0.208,P＜0.05).결론 ghrelin기인、Arg51 Gln、Leu72Met다태성여동향족、회족、한족2형당뇨병가능무관,단여동향족、회족혈지대사가능유관.
Objective To investigate the association of the ghrelin gene Arg51Gln and Leu72Met polymorphisms with type 2 diabetes meilitus and lipid metabolism in Dongxiang,Hui,Han population in Gansu province. Methods From October 2008 to February 2010,744 patients ( 386 males and 358 females) with type 2 diabetes mellitus( T2DM group) from Gansu Provincial People's Hospital,People's Hospital of Linxia and Dongxiang County Hospital were recruited,including 242 cases of Dongxiang nationality,242 cases of Hui nationality,260 cases of Han nationality.Total of 673 healthy volunteers from above 3 hospitals at the same time were regarded as the control group,including 223 Dongxiang nationality,203 Hui nationality and 247 Han nationality.Ghrelin gene Arg51Gln and Leu72Met polymorphisms were analyzed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Plasma glucose,lipids and other clinical indicators were detected.The genotype and allele frequency distribution were compared with the x2 test,while the correlation of genotype and plasma lipid levels were compared using correlation analysis and logistic regression analysis.Results ( 1 ) The carriers of A ( GA + AA) had a higher risk tendency in T2DM group than the non-carriers in Arg51Gln,and the carriers of A (CA + AA)had a higher risk tendency than the non-carriers in Leu72Met,but the differences were no statistical significance ( all OR ＞ 1,all P ＞ 0.05 ).No correlations were found in Arg51 Gln,Leu72Met polymorphisms with T2DM after adjusted the gender,age,body mass index(BMI) and blood pressure,etc( all P ＞0.05 ).(2)In the T2DM group,Arg51Gln genotype(GA + AA) in Dongxiang and Hui population was positively correlated with low-density lipoprotein cholesterol (LDL-C) level after adjusted for gender,age,BMI and total cholesterol(r =0.406,0.377,all P＜0.05).Leu72Met genotype(GA +AA) in Dongxiang and Hui population was positively correlated with total cholesterol level after adjusted for gender,age,BMI and LDL-C(r=0.189,0.208,all P＜0.05).Conclusions Arg51Gln,Leu72Met polymorphisms in ghrelin gene are probably unrelated to T2DM in Dongxiang,Hui and Han population,but might related to lipid metabolism in Dongxiang and Hui population.