CAJ | 학술논문

目的检测活性调节细胞骨架蛋白(Arc)在模型大鼠海马组织中的表达,探讨Arc与岛叶点燃大鼠学习记忆的关系.方法成年SD大鼠随机分为模型组、假手术组和空白对照组.应用避暗试验检测各组大鼠学习记忆功能.采用免疫组化、Western blot检测不同时间点Arc蛋白在海马组织中表达水平,运用RT-PGR检测其基因表达水平.结果慢性电点燃成功后第1天(即点燃0周),点燃组、假手术组及空白对照组学习记忆能力差异无统计学意义(P＞0.05).点燃2周:潜伏期(LT)(48.87±3.42)s,穿梭次数(EN)(2.75±0.99)次.点燃3周:LT为(48.71±2.77)s,EN为(2.75±1.15)次.点燃2周、3周与0周相比学习记忆功能增强(P＜0.05).点燃4周与0周组比学习记忆功能明显下降(P＜0.05).Arc在各组内蛋白及基因的表达均在1 h开始增加,6 h达到高峰,12 h恢复到基线水平.组间对比,2-3周内峰值最高,上升幅度明显(P＜0.01),4周峰值及幅度明显下降(P＜0.05),假手术组、空白对照组差异无统计学意义(P＞0.05).结论岛叶电点燃大鼠学习记忆功能及Arc在海马中表达变化趋势一致.Arc与岛叶点燃大鼠学习记忆功能密切相关.
목적 검측활성조절세포골가단백(Arc)재모형대서해마조직중적표체,탐토Arc여도협점연대서학습기억적관계.방법 성년SD대서수궤분위모형조、가수술조화공백대조조.응용피암시험검측각조대서학습기억공능.채용면역조화、Western blot검측불동시간점Arc단백재해마조직중표체수평,운용RT-PGR검측기기인표체수평.결과 만성전점연성공후제1천(즉점연0주),점연조、가수술조급공백대조조학습기억능력차이무통계학의의(P＞0.05).점연2주:잠복기(LT)(48.87±3.42)s,천사차수(EN)(2.75±0.99)차.점연3주:LT위(48.71±2.77)s,EN위(2.75±1.15)차.점연2주、3주여0주상비학습기억공능증강(P＜0.05).점연4주여0주조비학습기억공능명현하강(P＜0.05).Arc재각조내단백급기인적표체균재1 h개시증가,6 h체도고봉,12 h회복도기선수평.조간대비,2-3주내봉치최고,상승폭도명현(P＜0.01),4주봉치급폭도명현하강(P＜0.05),가수술조、공백대조조차이무통계학의의(P＞0.05).결론 도협전점연대서학습기억공능급Arc재해마중표체변화추세일치.Arc여도협점연대서학습기억공능밀절상관.
Objective To research the expression of activity- regulated cytoskeletal protein in Insula Chronic Electricity igniting epilepsy rat's hippocampus,and to study the relation between Arc and electricity igniting epilepsy rat's learning and memory.Methods Adult SD rats were divided into model groups,sham operation groups,blank control groups.The model of rat insula chronic electricity igniting was built,and rats learning and memory ability were examined by way of passive avoidance test.The expression level of Arc in hippocampus was test by both immunohistochemistry and western blot,and the expression of Arc gene was test by RT- PCR.ResultThere was no significant difference of learning- remembering ability of rats between igniting group,control group and sham groups (P ＞ 0.05) in the first week after insular chronic ignition when passive avoidance test was examined.The increased learning- remembering ability appeared in the 2nd week (LT:48.87 ± 3.42 s; EN:2.75 ± 0.99) ,3rd week(LT:48.71 ± 2.77 s;EN:2.75 ± 1.15) after the ignition(P ＜0.05).After 4 weeks,there was obvious decline of learn and memory (P<0.05 ).When testing the molecular biology of Arc,there was an enhancement in expression of protein and gene for Arc in subgroup time point from 1 h,to the submit when 6 h,and to primary level when 12 h.The general tendency was to rise when one week(w),to rise fast when 2w-3w,to rise obviously slowly when 4w(P ＜0.05).There was no sense for false operation sets and blank comparison sets.Conclusion The general tendency of the expression of Arc in rat hippocampus after the chronic insular electricity ignition is consistent with the learning- remembering ability of epilepsy rats.Arc is closely associated with the learning- remember ability for epilepsy rats.