中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2011年
3期
150-154
,共5页
陈志涛%周峰%黄莎%陈立平%姜挺%邹开芳%夏冰
陳誌濤%週峰%黃莎%陳立平%薑挺%鄒開芳%夏冰
진지도%주봉%황사%진립평%강정%추개방%하빙
细胞毒T淋巴细胞相关抗原4%克罗恩病%红细胞沉降率%C反应蛋白
細胞毒T淋巴細胞相關抗原4%剋囉恩病%紅細胞沉降率%C反應蛋白
세포독T림파세포상관항원4%극라은병%홍세포침강솔%C반응단백
Cytotoxic T-lymphocyte antigen 4%Crohn's disease%Blood sedimentation: C reactive protein
目的 研究可溶性细胞毒T淋巴细胞相关抗原4(sCTLA4)在克罗恩病(CD)患者血清中的表达状况,分析sCTLA4水平与红细胞沉降率(ESR)和C反应蛋白(CRP)的关系及其临床意义,探讨CTLA4基因-1661A/G和-1722T/C多态性与CD的相关性.方法 收集126例CD患者和300名健康对照者,酶联免疫吸附实验测定血清sCTLA4水平.全自动红细胞沉降系统分析仪测定ESR水平.速率散射比浊法测定CRP水平.DNA测序技术检测CTLA4基因-1661A/G和-1722T/C多态性.结果 CD患者血清中sCTLA4水平为(18.70±3.72)ng/ml,明显高于健康对照者[(1.72±0.32)ng/ml,P＜0.01].活动期CD患者血清sCTLA4水平为(19.83±4.35)ng/ml,比缓解期CD患者[(18.02±3.14)ng/ml,P=0.015]增高.CD患者血清sCTLA4水平与ESR和CRP水平呈正相关(r=0.267,P=0.003;r=0.524,P＜0.01).疾病行为影响CD患者血清sCTLA4水平,狭窄型CD患者血清sCTLA4水平高于非狭窄非穿透型和穿透型CD患者,差异均有统计学意义(P值分别=0.021和0.015).CD组和健康对照组中CTLA4基因-1661A/G,-1722T/C等位基因及基因型频率差异无统计学意义(P＞0.05).结论 CD患者血清sCTLA4水平增高,增高的sCTLA4水平不仅与疾病活动状态及狭窄型疾病行为相关,且与CRP正相关,提示sCTLA4分子在CD免疫学发病中可能起重要作用.
目的 研究可溶性細胞毒T淋巴細胞相關抗原4(sCTLA4)在剋囉恩病(CD)患者血清中的錶達狀況,分析sCTLA4水平與紅細胞沉降率(ESR)和C反應蛋白(CRP)的關繫及其臨床意義,探討CTLA4基因-1661A/G和-1722T/C多態性與CD的相關性.方法 收集126例CD患者和300名健康對照者,酶聯免疫吸附實驗測定血清sCTLA4水平.全自動紅細胞沉降繫統分析儀測定ESR水平.速率散射比濁法測定CRP水平.DNA測序技術檢測CTLA4基因-1661A/G和-1722T/C多態性.結果 CD患者血清中sCTLA4水平為(18.70±3.72)ng/ml,明顯高于健康對照者[(1.72±0.32)ng/ml,P＜0.01].活動期CD患者血清sCTLA4水平為(19.83±4.35)ng/ml,比緩解期CD患者[(18.02±3.14)ng/ml,P=0.015]增高.CD患者血清sCTLA4水平與ESR和CRP水平呈正相關(r=0.267,P=0.003;r=0.524,P＜0.01).疾病行為影響CD患者血清sCTLA4水平,狹窄型CD患者血清sCTLA4水平高于非狹窄非穿透型和穿透型CD患者,差異均有統計學意義(P值分彆=0.021和0.015).CD組和健康對照組中CTLA4基因-1661A/G,-1722T/C等位基因及基因型頻率差異無統計學意義(P＞0.05).結論 CD患者血清sCTLA4水平增高,增高的sCTLA4水平不僅與疾病活動狀態及狹窄型疾病行為相關,且與CRP正相關,提示sCTLA4分子在CD免疫學髮病中可能起重要作用.
목적 연구가용성세포독T림파세포상관항원4(sCTLA4)재극라은병(CD)환자혈청중적표체상황,분석sCTLA4수평여홍세포침강솔(ESR)화C반응단백(CRP)적관계급기림상의의,탐토CTLA4기인-1661A/G화-1722T/C다태성여CD적상관성.방법 수집126례CD환자화300명건강대조자,매련면역흡부실험측정혈청sCTLA4수평.전자동홍세포침강계통분석의측정ESR수평.속솔산사비탁법측정CRP수평.DNA측서기술검측CTLA4기인-1661A/G화-1722T/C다태성.결과 CD환자혈청중sCTLA4수평위(18.70±3.72)ng/ml,명현고우건강대조자[(1.72±0.32)ng/ml,P＜0.01].활동기CD환자혈청sCTLA4수평위(19.83±4.35)ng/ml,비완해기CD환자[(18.02±3.14)ng/ml,P=0.015]증고.CD환자혈청sCTLA4수평여ESR화CRP수평정정상관(r=0.267,P=0.003;r=0.524,P＜0.01).질병행위영향CD환자혈청sCTLA4수평,협착형CD환자혈청sCTLA4수평고우비협착비천투형화천투형CD환자,차이균유통계학의의(P치분별=0.021화0.015).CD조화건강대조조중CTLA4기인-1661A/G,-1722T/C등위기인급기인형빈솔차이무통계학의의(P＞0.05).결론 CD환자혈청sCTLA4수평증고,증고적sCTLA4수평불부여질병활동상태급협착형질병행위상관,차여CRP정상관,제시sCTLA4분자재CD면역학발병중가능기중요작용.
Objective To investigate the expression of serum soluble cytotoxic T lymphocyte associated antigen 4 (sCTLA4), the association of sCTLA4 level with erythrocyte sedimentation rate (ESR) and C reactive protein (CRP), as well as its role in patients with Crohn's disease (CD). The relationship-1661A/G and -1722T/C polymorphisms of CTLA4 gene and between disease susceptibility and phenotype of CD was analyzed. Methods A total of 126 CD patients and 300 healthy controls were enrolled in the study. Serum sCTLA4 level was determined by enzyme-linked immunosorbent assay. The concentrations of ESR and CRP were analyzed by automatic ESR Analyzer SRS 100/Ⅱ and rate nephelometry, respectively. The polymorphisms of CTLA4-1661A/G and -1722 T/C were genotyped by DNA sequencing. Results Serum sCTLA4 level was higher in CD patients than in healthy controls [(18. 70±3. 72) ng/ml vs (1.72±0. 32) ng/ml, P＜0. 01)]. Among CD patients, sCTLA4 level was higher in patients with active disease when compared to those with inactive disease [(19.83±4.35) ng/ml vs (18. 02±3.14) ng/ml, P=0. 015)]. sCTLA4 level was positively correlated with ESR and CRP levels (r=0. 267, P=0. 003; r=0. 524 P ＜0.01, respectively). In CD patients, serum sCTLA4 level was significantly higher in those with stricturing disease behavior than that in those without stricturing and penetrating or with penetrating disease behavior (P= 0.021; P=0. 015, respectively). Detection of CTLA4 -1661A/G and -1722T/C polymorphisms showed no significant difference between CD patients and healthy controls. Conclusions The high level of serum sCTLA4 in CD patients is correlated with disease activity, CRP levels and disease behavior. It suggests that sCTLA4 may play an important role in pathogenesis of CD.
