CAJ | 학술논문

目的探讨过氧化物酶体增殖物激活受体(PPAR)γ激动剂吡咯列酮在雨蛙肽诱导大鼠急性胰腺炎中对氧化应激产物的影响及保护作用.方法 30只雄性SD大鼠随机分为对照组、雨蛙肽+不同剂量吡咯列酮组、雨蛙肽组、雨蛙肽+吡咯列酮+GW9662组.每组6只.急性胰腺炎造模30 min后处死大鼠,光镜下观察胰腺组织病理学变化,测定各组大鼠胰腺组织质量与体重比,比色法检测胰腺组织髓过氧化物酶(MPO)活性、丙二醛(MDA)和一氧化氮合酶(NOS)及组织诱导型一氧化氮合酶(iNOS)含量.结果与对照组比较,雨蛙肽组胰腺组织水肿严重胰腺净重/体重(0.0072比0.0042)],MPO活性、MDA、NOS及iNOS含量升高(P＜0.01).与雨蛙肽组比较,吡咯列酮20 mg/kg及40 mg/kg组胰腺损伤减轻,胰腺净重/体重、MPO活性、MDA和NOS及iNOS含量降低(P＜0.05);与吡咯列酮40 mg/kg组比较,PPARγ拮抗剂GW9662逆转了吡咯列酮的保护作用(P＜0.05).结论在雨蛙肽诱导的大鼠急性胰腺炎发病中,胰腺腺泡细胞的氧化应激损伤起了重要的作用,PPARγ激动剂吡咯列酮预先干预,通过降低氧化应激过程,对雨蛙肽诱导的大鼠急性胰腺炎有一定的保护作用.
목적 탐토과양화물매체증식물격활수체(PPAR)γ격동제필각렬동재우와태유도대서급성이선염중대양화응격산물적영향급보호작용.방법 30지웅성SD대서수궤분위대조조、우와태+불동제량필각렬동조、우와태조、우와태+필각렬동+GW9662조.매조6지.급성이선염조모30 min후처사대서,광경하관찰이선조직병이학변화,측정각조대서이선조직질량여체중비,비색법검측이선조직수과양화물매(MPO)활성、병이철(MDA)화일양화담합매(NOS)급조직유도형일양화담합매(iNOS)함량.결과 여대조조비교,우와태조이선조직수종엄중이선정중/체중(0.0072비0.0042)],MPO활성、MDA、NOS급iNOS함량승고(P＜0.01).여우와태조비교,필각렬동20 mg/kg급40 mg/kg조이선손상감경,이선정중/체중、MPO활성、MDA화NOS급iNOS함량강저(P＜0.05);여필각렬동40 mg/kg조비교,PPARγ길항제GW9662역전료필각렬동적보호작용(P＜0.05).결론 재우와태유도적대서급성이선염발병중,이선선포세포적양화응격손상기료중요적작용,PPARγ격동제필각렬동예선간예,통과강저양화응격과정,대우와태유도적대서급성이선염유일정적보호작용.
Objective To investigate the effects of pioglitazone,a peroxisome proliferation activated receptor(PPAR)γ agonist on oxidative stress process and the therapeutic effects on severity of acute pancreatitis(AP).Methods Thirty male Sprague-Dawley rats were randomly divided into five groups with 6 in each:control group,eerulein plus pioglitazone group,cerulein group,cerulein plus vehicle group,cerulein plus pioglitazone and GW9662 group.Rats were sacrificed at 30 min after the induction of pancreatitis.Pathologic changes of the pancreas were observed under light microscope.The ratios of pancreatic wet/body weight of rat were determined in each group.Serum amylase,pancreatic nitric oxide synthase(NOS),inducible nitric oxide synthase(iNOS),malondialdehyde(MDA),and myeloperoxidase (MPO)were determined by chromometry.Results The serum amylase,pancreatic wet/body weight and the score of pathologic damage increased after the induction of pancreatitis,AP samples were characterized by increased pancreatic MDA,MPO,NOS and iNOS(P＜0.01).Pioglitazone(20 mg/kg and 40 mg/kg)exhibited a protective effect against oxygen free radicals reflected by lower serum amylase,less severe pancreatic lesions,normal pancreatic MDA,MPO and NOS levels(P＜0.05).GW9662 reversed the effects against oxidative stress of pioglitazone(40 mg/kg)(P＜0.05).Conclusions Pretreatment with pioglitazone may exert its therapeutic effect on AP by lowering pancreatic oxidative free radicals and reducing pancreatic tissue infiltration of neutrophils.