CAJ | 학술논문

目的探讨Pyrin重组蛋白对支气管哮喘小鼠气道炎症的抑制作用及机制的研究.方法 40只雄性清洁级BALB/c小鼠,随机分为4组,每组10只.分别为生理盐水对照组、卵蛋白(OVA)哮喘组、Pyrin重组蛋白治疗3d组和Pyrin重组蛋白治疗7d组.在末次激发24h后所有小鼠取左肺组织行苏木精-伊红(HE)染色,Masson三色染色;用图像分析软件测定支气管壁厚度(WAt/Pbm)、支气管平滑肌厚度(WAm/Pbm)及胶原纤维面积;用酶联免疫吸附法( ELISA)检测支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中IL-4、IL-5、TNF-α及IFN-γ含量;用RT-PCR检测肺组织中结缔组织生长因子(connective tissue growth factor,CTGF)和转化生长因子-β1(transforming growth factor-β1,TGF-β1)的mRNA表达;用Western blot方法检测核转录因子(nuclear factor kappa B,NF-kB)的表达.结果哮喘模型组小鼠BALF中细胞总数、IL-4、IL-5、TNF-α水平增高和IFN-γ水平降低;肺组织支气管管壁厚度、气道平滑肌厚度、胶原纤维面积,CTGF、TGF-β1的转录和表达水平以及NF-kB表达水平均显著高于生理盐水对照组(P＜0.05).Pyrin重组蛋白3d和7d干预组小鼠BALF中细胞总数、IL-4、IL-5、TNF-α水平和肺组织支气管管壁厚度、气道平滑肌厚度、胶原纤维面积、NF-kB表达水平显著降低,与哮喘模型组比较差异均具有统计学意义(P＜0.05),而BALF中IFN-γ水平明显上升,与哮喘模型组比较,差异均具有统计学意义(P＜0.05);Pyrin重组蛋白7d干预组CTGF、TGF-β1的转录和表达水平显著低于哮喘组(P＜0.05).结论我们推测Pyrin重组蛋白可能部分是通过抑制NF-kB信号途径来阻断TGF-β1和CTGF的过度表达而实现抑制气道炎症,进而抑制气道重构的发生.
목적 탐토Pyrin중조단백대지기관효천소서기도염증적억제작용급궤제적연구.방법 40지웅성청길급BALB/c소서,수궤분위4조,매조10지.분별위생리염수대조조、란단백(OVA)효천조、Pyrin중조단백치료3d조화Pyrin중조단백치료7d조.재말차격발24h후소유소서취좌폐조직행소목정-이홍(HE)염색,Masson삼색염색;용도상분석연건측정지기관벽후도(WAt/Pbm)、지기관평활기후도(WAm/Pbm)급효원섬유면적;용매련면역흡부법( ELISA)검측지기관폐포관세액(bronchoalveolar lavage fluid,BALF)중IL-4、IL-5、TNF-α급IFN-γ함량;용RT-PCR검측폐조직중결체조직생장인자(connective tissue growth factor,CTGF)화전화생장인자-β1(transforming growth factor-β1,TGF-β1)적mRNA표체;용Western blot방법검측핵전록인자(nuclear factor kappa B,NF-kB)적표체.결과 효천모형조소서BALF중세포총수、IL-4、IL-5、TNF-α수평증고화IFN-γ수평강저;폐조직지기관관벽후도、기도평활기후도、효원섬유면적,CTGF、TGF-β1적전록화표체수평이급NF-kB표체수평균현저고우생리염수대조조(P＜0.05).Pyrin중조단백3d화7d간예조소서BALF중세포총수、IL-4、IL-5、TNF-α수평화폐조직지기관관벽후도、기도평활기후도、효원섬유면적、NF-kB표체수평현저강저,여효천모형조비교차이균구유통계학의의(P＜0.05),이BALF중IFN-γ수평명현상승,여효천모형조비교,차이균구유통계학의의(P＜0.05);Pyrin중조단백7d간예조CTGF、TGF-β1적전록화표체수평현저저우효천조(P＜0.05).결론 아문추측Pyrin중조단백가능부분시통과억제NF-kB신호도경래조단TGF-β1화CTGF적과도표체이실현억제기도염증,진이억제기도중구적발생.
Objective To investigate the blocking effects of Pyrin domain protein on mouse allergic airway inflammation induced by ovalbumin and its mechanism.Methods Forty male BALB/c mice were randomly divided into 4 groups with 10 mice in each group.Control group:mice were treated with saline;OVA group:mice were sensitized and challenged with OVA; Pyrin domain protein 3 d group:mice were sensitized and challenged as asthmatic group and treated with 100 μg/kg triptolide before challenged; Pyrin domain protein 7 d group:mice were sensitized and challenged as asthmatic group and treated with 100 μg/kg triptolide before challenged.All mice were killed 24 h after final OVA challenge.The left lung was isolated for pathological examination.Lung sections were stained with hematoxylin and eosin(HE),Masson's trichrome.The thickness of bronchial airway,bronchial smooth muscle thickness,and the collagen deposition area were measured by image analysig system.The concentrations of IL-4,IL-5,TNF-α and IFN-γ in BALF were measured by ELISA,the RT-PCR and Western blot was performed to detect the mRNA expression of connective tissue growth factor (CTGF),TGF-β 1 mRNA and NF -kB from the right lung tissues.Results In OVA group,the number of inflammatory cells and the concentrations of IL-4,IL-5,TNF-a in BALF and Bronchial airway thickness,bronchial smooth muscle thickness,the collagen deposition area,CTGF,TGF-β1 mRNA and NF-kB in lung tissue were significantly higher than those in control group(P＜0.05).IFN-γ were lower than those in control group( P＜0.05 ).While in Pyrin domain protein 3 d group and Pyrin domain protein 7 d group,the number of inflammatory cells,the concentrations of IL-4,IL-5,TNF-α in BALF and bronchial airway thickness,bronchial smooth muscle thickness,the collagen deposition area and NF-kB in lung tissue were significantly lower than those in OVA group,IFN-γwere higher than those in OVA group( P ＜0.05).In Pyrin domain protein 7 d group CTGF and TGF-β1 mRNA in lung tissue were significantly lower than those in OVA group.Conclusion Pyrin domain protein might inhibits airway inflammation and development of airway remodeling in asthmatic mouse,the possible mechanism might in part by associated with reducing the expression of NF-kB and further inhibiting CTGF,TGF-β1.