CAJ | 학술논문

目的探讨人体组织生物样本库建设,标本收集、保存、质最控制、管理及应用等有关问题.方法制定标准化操作流程,收取配套新鲜冷冻组织、石蜡包埋组织、全血、血清、血浆、脑脊液等标本,按不同要求处理分装,常规、液氮或-80℃超低温冰箱冷冻保存,应用数据库管理系统进行信息化管理.结果从2004年9月至2008年9月共收集各类疾病标本504个病种,11872例33137管份,至今已向省内外提供新鲜冷冻组织标本2500例4360管,应用于DNA、RNA、蛋白提取及组织芯片、免疫组织化学等的相关性研究.结论人体组织生物样本库的建立,有利于有效利用不可再生的人体组织标本资源,随时提供符合要求的良恶性疾病标本和正常对照标本;有利于探讨疾病的致病机制、开发疾病早期榆测新技术和新的治疗策略.
목적 탐토인체조직생물양본고건설,표본수집、보존、질최공제、관리급응용등유관문제.방법 제정표준화조작류정,수취배투신선냉동조직、석사포매조직、전혈、혈청、혈장、뇌척액등표본,안불동요구처리분장,상규、액담혹-80℃초저온빙상냉동보존,응용수거고관리계통진행신식화관리.결과 종2004년9월지2008년9월공수집각류질병표본504개병충,11872례33137관빈,지금이향성내외제공신선냉동조직표본2500례4360관,응용우DNA、RNA、단백제취급조직심편、면역조직화학등적상관성연구.결론 인체조직생물양본고적건립,유리우유효이용불가재생적인체조직표본자원,수시제공부합요구적량악성질병표본화정상대조표본;유리우탐토질병적치병궤제、개발질병조기유측신기술화신적치료책략.
Objective To explore issues of the human tissue biobank, ranging from its establishment, collection and preservation of samples, quality control, management and application. Methods Development of standardized operational procedures, collection of such samples as fresh frozen tissues from the surgery, paraffin-embedded tissues, whole blood, serum, plasma, and cerebrospinal fluid. Specimens were classified and made aliquots according to different requirements, and then stored at room temperature, -80℃ refrigerator or in liquid nitrogen. Microsoft Access database system was used in the management of these specimens. Results From September 2004 to September 2008, a total of 11 872 samples from patients with benign and malignant diseases were collected and preserved. Among them, 4360 tubes of fresh frozen tissue samples from 2500 cases were provided within and beyond the province. These samples were also applied to DNA, RNA, protein extraction and tissue microarray, and immunohistochemistry-related research. Conclusion Human tissue biobank is highly useful in sharing human tissue resources effectively, as it can provide high quality specimens from benign and malignant diseases and normal control. In addition, it plays a very important role in exploring pathogenesis, developing new technologies for early detection of disease and new therapeutic strategies.