中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2008年
3期
193-196
,共4页
熊舒原%曹宁%察鹏飞%卓双木%陈建新
熊舒原%曹寧%察鵬飛%卓雙木%陳建新
웅서원%조저%찰붕비%탁쌍목%진건신
成纤维细胞%细胞移植%双光子显微镜
成纖維細胞%細胞移植%雙光子顯微鏡
성섬유세포%세포이식%쌍광자현미경
Fibroblasts%Cell transplantation%Two-photon fluorescence microscopy
目的 通过双光子显微镜观察体外培养的小鼠皮肤成纤维细胞皮内注射移植后的长期存活情况,了解胶原纤维等基质成分的变化.方法 将增强型绿色荧光蛋白(EGFP)慢病毒液转染成功的成纤维细胞注射到小鼠皮内,分别于注射1、2个月后取材,行连续冰冻切片,双光子显微镜观察,对胶原的分布面积和真皮厚度做图像分析,并对所得数据进行统计学处理.结果 双光子显微镜对注射移植细胞及胶原清晰成像,注射1个月时,胶原分布面积及真皮厚度与对照组比较差异无统计学意义(P>0.05);2个月后胶原分布面积及真皮厚度与对照组比较差异有统计学意义(P<0.01).结论 成纤维细胞注射移植到小鼠皮内可以长期存活,能够分泌胶原并增加真皮厚度,为临床上应用自体真皮成纤维细胞进行软组织填充注射治疗的可行性,提供了理论基础.
目的 通過雙光子顯微鏡觀察體外培養的小鼠皮膚成纖維細胞皮內註射移植後的長期存活情況,瞭解膠原纖維等基質成分的變化.方法 將增彊型綠色熒光蛋白(EGFP)慢病毒液轉染成功的成纖維細胞註射到小鼠皮內,分彆于註射1、2箇月後取材,行連續冰凍切片,雙光子顯微鏡觀察,對膠原的分佈麵積和真皮厚度做圖像分析,併對所得數據進行統計學處理.結果 雙光子顯微鏡對註射移植細胞及膠原清晰成像,註射1箇月時,膠原分佈麵積及真皮厚度與對照組比較差異無統計學意義(P>0.05);2箇月後膠原分佈麵積及真皮厚度與對照組比較差異有統計學意義(P<0.01).結論 成纖維細胞註射移植到小鼠皮內可以長期存活,能夠分泌膠原併增加真皮厚度,為臨床上應用自體真皮成纖維細胞進行軟組織填充註射治療的可行性,提供瞭理論基礎.
목적 통과쌍광자현미경관찰체외배양적소서피부성섬유세포피내주사이식후적장기존활정황,료해효원섬유등기질성분적변화.방법 장증강형록색형광단백(EGFP)만병독액전염성공적성섬유세포주사도소서피내,분별우주사1、2개월후취재,행련속빙동절편,쌍광자현미경관찰,대효원적분포면적화진피후도주도상분석,병대소득수거진행통계학처리.결과 쌍광자현미경대주사이식세포급효원청석성상,주사1개월시,효원분포면적급진피후도여대조조비교차이무통계학의의(P>0.05);2개월후효원분포면적급진피후도여대조조비교차이유통계학의의(P<0.01).결론 성섬유세포주사이식도소서피내가이장기존활,능구분비효원병증가진피후도,위림상상응용자체진피성섬유세포진행연조직전충주사치료적가행성,제공료이론기출.
Objective To investigate the survival profile of the intradermally injected mouse autologous skin fibroblasts and the changes of the collagen fibers by using green fluorescent protein labeling and two-photon fluorescence microscopy. Methods The cultured cells were transfected by EGFP lentivirus, and then the cells were injected into the corresponding mouse skin. Biopsy was taken from the animals after 1 and 2 months. The specimens made serial frozen sections, the survival profile of the injected cells and the changes of the collagen fibers were observed by two-photon fluorescence microscopy. The collagenic area and dermal thickness were measured with image analysis software, and statistical analysis was also carried out. Results Two-photon fluorescence microscopy showed clear images of the injected cells and collagen fibers. Both the area of collagen fibers and the dermal thickness were significantly increased in injected cells after 2 months (P<0.05), however, there were no difference between injected cells and control after 1 mouth (P>0.05). Conclusions Autologous cultured fibroblasts could survive in a long time after transplantating into the skin, and collagen could be newly produced, the depth of dermis increases, which provides a possibility to treat mini-defects of the tissue.