中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2012年
2期
132-136
,共5页
刘勇%谢丛良%邱元正%田勇泉%张欣%黄东海%周小娟%谭平清%余长云%柒琳%李波%肖健云
劉勇%謝叢良%邱元正%田勇泉%張訢%黃東海%週小娟%譚平清%餘長雲%柒琳%李波%肖健雲
류용%사총량%구원정%전용천%장흔%황동해%주소연%담평청%여장운%칠림%리파%초건운
喉肿瘤%肿瘤,鳞状细胞%HMGB1蛋白%肿瘤转移%预后
喉腫瘤%腫瘤,鱗狀細胞%HMGB1蛋白%腫瘤轉移%預後
후종류%종류,린상세포%HMGB1단백%종류전이%예후
Laryngeal neoplasms%Neoplasms,squamous cell%HMGB1 protein%Neoplasm metastasis%Prognosis
目的 检测高迁移率族蛋白1( HMGB1)在喉鳞状细胞癌(简称喉鳞癌)组织中的表达,并探讨其表达与喉鳞癌临床病理特征和预后之间的关系.方法 采用免疫组织化学技术检测HMGB1蛋白在93例喉鳞癌和15例癌旁黏膜组织中的表达,并分析其表达水平与临床病理特征和预后的关系.结果 HMGB1蛋白在喉鳞癌组织中的阳性率(87.1％)显著高于癌旁黏膜中的阳性率(46.7％,P=0.001),且其表达水平与喉鳞癌的T分期(x2=10.878,P=0.004)、临床分期(x2=21.115,P＜0.01)、转移(x2=28.298,P＜0.01)及复发(x2=14.923,P=0.001)密切相关.KaplanMeier生存分析显示,HMGB1高表达组、中表达组和低表达组患者的5年无瘤生存率分别为27.8％、43.9％和91.7％,5年总生存率分别为38.9％、68.3％和91.7％,差异均有统计学意义(x2=13.815,P=0.001；x2=11.912,P=0.003).单因素及多因素Cox比例风险回归模型分析显示,HMGB1蛋白表达水平和淋巴结转移情况为喉鳞癌患者预后的独立影响因素.结论 HMGB1蛋白在喉鳞癌组织中表达显著升高,且其高表达与喉鳞癌患者的预后密切相关.HMGB1可能在喉鳞癌的发生、发展中有重要作用,有可能成为评估喉鳞癌患者预后的重要分子标志物.
目的 檢測高遷移率族蛋白1( HMGB1)在喉鱗狀細胞癌(簡稱喉鱗癌)組織中的錶達,併探討其錶達與喉鱗癌臨床病理特徵和預後之間的關繫.方法 採用免疫組織化學技術檢測HMGB1蛋白在93例喉鱗癌和15例癌徬黏膜組織中的錶達,併分析其錶達水平與臨床病理特徵和預後的關繫.結果 HMGB1蛋白在喉鱗癌組織中的暘性率(87.1％)顯著高于癌徬黏膜中的暘性率(46.7％,P=0.001),且其錶達水平與喉鱗癌的T分期(x2=10.878,P=0.004)、臨床分期(x2=21.115,P＜0.01)、轉移(x2=28.298,P＜0.01)及複髮(x2=14.923,P=0.001)密切相關.KaplanMeier生存分析顯示,HMGB1高錶達組、中錶達組和低錶達組患者的5年無瘤生存率分彆為27.8％、43.9％和91.7％,5年總生存率分彆為38.9％、68.3％和91.7％,差異均有統計學意義(x2=13.815,P=0.001；x2=11.912,P=0.003).單因素及多因素Cox比例風險迴歸模型分析顯示,HMGB1蛋白錶達水平和淋巴結轉移情況為喉鱗癌患者預後的獨立影響因素.結論 HMGB1蛋白在喉鱗癌組織中錶達顯著升高,且其高錶達與喉鱗癌患者的預後密切相關.HMGB1可能在喉鱗癌的髮生、髮展中有重要作用,有可能成為評估喉鱗癌患者預後的重要分子標誌物.
목적 검측고천이솔족단백1( HMGB1)재후린상세포암(간칭후린암)조직중적표체,병탐토기표체여후린암림상병리특정화예후지간적관계.방법 채용면역조직화학기술검측HMGB1단백재93례후린암화15례암방점막조직중적표체,병분석기표체수평여림상병리특정화예후적관계.결과 HMGB1단백재후린암조직중적양성솔(87.1％)현저고우암방점막중적양성솔(46.7％,P=0.001),차기표체수평여후린암적T분기(x2=10.878,P=0.004)、림상분기(x2=21.115,P＜0.01)、전이(x2=28.298,P＜0.01)급복발(x2=14.923,P=0.001)밀절상관.KaplanMeier생존분석현시,HMGB1고표체조、중표체조화저표체조환자적5년무류생존솔분별위27.8％、43.9％화91.7％,5년총생존솔분별위38.9％、68.3％화91.7％,차이균유통계학의의(x2=13.815,P=0.001；x2=11.912,P=0.003).단인소급다인소Cox비례풍험회귀모형분석현시,HMGB1단백표체수평화림파결전이정황위후린암환자예후적독립영향인소.결론 HMGB1단백재후린암조직중표체현저승고,차기고표체여후린암환자적예후밀절상관.HMGB1가능재후린암적발생、발전중유중요작용,유가능성위평고후린암환자예후적중요분자표지물.
Objective To evaluate the expression of HMGB1 protein in tissue specimens of laryngeal squamous cell carcinoma (LSCC) and adjacent normal mucosa,and explore the correlation of HMGB1 protein expression with clinicopathologic features and prognosis in LSCC.Methods Ninty-three cases of LSCC and 15 cases of adjcent mucosal tissue samples were included in this study.Immunohistochemical staining was performed on paraffin-embedded tissue specimens to examine the HMGB1 protein expression.The data were futher correlated with the clinicopathological features and prognosis of the LSCC patients.Results The positive rates of HMGB1 expression in LSCC specimens was 87.1％,significantly higher than that in the adjcent normal mucosa samples ( 46.7％,P =0.001 ),and its overexpresion was closely correlated with T stage (x2 =10.878,P =0.004),clinical stage (x2 =21.115,P ＜0.01 ),metastasis (x2 =28.298,P ＜0.0l ) and recurrence (x2 =14.923,P =0.001 ) in patients with LSCC.Patients with HMGB1 overexpression had both poorer disease-free survival and poorer overall survival compared with that in patients with low HMGB1 expression (x2 =13.815,x2 =11.912 ; Both P ＜ 0.01 ).Univariate and multivariate Cox regression analyses revealed that HMGB1 expression is an independent prognostic factor for patients with LSCC.Conclusions The results of this study demonstrate that HMGB1 protein expression is significantly increased in LSCC tissues,and HMGB1 protein overexpression is associated with a poorer prognosis in patients with LSCC.These results suggest that HMGB1 may play a critical role in the initiation and progression of LSCC,implicating HMGB1 may become a valuable marker for the prediction of prognosis in patients with LSCC.
