CAJ | 학술논문

采用分光光度法测定了乌蕨3种不同提取液对超氧阴离子自由基(O_2~-·)和羟自由基(·OH)的清除作用大小.结果表明水提液、质量分数为50%的乙醇提取液和质量分数为80%的丙酮提取液在干样浓度为10.00～50.00 μg·mL~(-1)时,对羟自由基的清除率为:水提液(36.85%)>乙醇(20.61%)>丙酮(18.70%);对超氧自由基的清除率为:丙酮(31.77%)>水提液(31.03%)>乙醇(24.37%).还利用超声提取法对乌蕨水提液的抗氧化能力进行了研究,结果表明超声水提液对超氧自由基和羟自由基的清除率均大于回流提取液.
채용분광광도법측정료오궐3충불동제취액대초양음리자자유기(O_2~-·)화간자유기(·OH)적청제작용대소.결과표명수제액、질량분수위50%적을순제취액화질량분수위80%적병동제취액재간양농도위10.00～50.00 μg·mL~(-1)시,대간자유기적청제솔위:수제액(36.85%)>을순(20.61%)>병동(18.70%);대초양자유기적청제솔위:병동(31.77%)>수제액(31.03%)>을순(24.37%).환이용초성제취법대오궐수제액적항양화능력진행료연구,결과표명초성수제액대초양자유기화간자유기적청제솔균대우회류제취액.
Scavenging effects of Stenoloma Chusana(L.) Ching on hydroxyl radicals(·OH) and superoxide anion radicals(O_2~-·) of different extracts are studied systemically by spectrophotometry. The water extract, theφ(ethanol)=50% extracts and the φ(acetone)=80% extract have the scavenging effect on hydroxyl radical and superoxide anion radical. Its consistence ranges are 10.00～50.00 μg·mL~(-1). The scavenging effects on ·OH are: water (36.85%)>ethanol (20.61%)> acetone (18.70%), and ones on O_2~-· are: acetone (31.77%)>water (31.03%)>ethanol (24.37%). The antioxygenic activity of water extract is also studied by the ultrasonic wave extraction. The results show that ultrasonic extraction is a highly efficient extraction method compared with the reflux extraction.