CAJ | 학술논문

目的观察鱼藤酮对大鼠中脑α-突触核蛋白(α-SYN)的影响.方法 SD大鼠长期低剂量皮下注射1.0 mg·kg~(-1)·d~(-1)的鱼藤酮,建立帕金森病(PD)动物模型.采用旷场实验评价动物行为学改变,观察大鼠5 min的活动情况.免疫荧光化学方法检测中脑部位TH阳性神经元数目的变化.免疫组织化学和Western blot方法检测中脑部位α-SYN的蛋白水平的改变.结果同对照组相比,鱼藤酮实验组动物5 min的正中格停留时间明显延长(P<0.05),方格穿行次数明显减少(P<0.05),竖起时间和次数明显减少(P<0.01);中脑部位TH阳性神经元数目明显减少(P<0.05),α-SYN蛋白水平明显增加(P<0.05).结论给予鱼藤酮能够使SD大鼠出现运动障碍,中脑部位TH阳性神经元数目减少,该现象可能与大鼠中脑部位α-SYN蛋白水平增加有关.
목적 관찰어등동대대서중뇌α-돌촉핵단백(α-SYN)적영향.방법 SD대서장기저제량피하주사1.0 mg·kg~(-1)·d~(-1)적어등동,건립파금삼병(PD)동물모형.채용광장실험평개동물행위학개변,관찰대서5 min적활동정황.면역형광화학방법검측중뇌부위TH양성신경원수목적 변화.면역조직화학화Western blot방법검측중뇌부위α-SYN적단백수평적개변.결과 동대조조상비,어등동실험조동물5 min적정중격정류시간명현연장(P<0.05),방격천행차수명현감소(P<0.05),수기시간화차수명현감소(P<0.01);중뇌부위TH양성신경원수목명현감소(P<0.05),α-SYN단백수평명현증가(P<0.05).결론 급여어등동능구사SD대서출현운동장애,중뇌부위TH양성신경원수목감소,해현상가능여대서중뇌부위α-SYN단백수평증가유관.
Abstract:Aim To study the effect of rotenone on α-synuclein in rat midbrain of Parkinson's disease(PD).Methods Rats were subcutaneouly injected with chronic low dose rotenone(1.0 mg·kg~(-1)·d~(-1)).Movements within 5 minutes were evaluated in open field test.Tyrosine hydroxylase(TH)-positive neuronsin the midbrain were measured with immunofluorescence staining.α-SYN protein level in the midbrain was demonstrated with immunohistochemical staining and Western blot.Results Compared to the control group,latency time,crossing,rearing and rearing time were changed significantly(P<0.05,0.01)in the rotenone group,TH-positive neurons were reduced(P<0.05)and α-SYN protein level in the midbrain was increased(P<0.05).Conclusion Injection with rotenone can induce PD symptom,which may be correlated to α-SYN protein level in the midbrain.