CAJ | 학술논문

目的观察快速老化小鼠P8(SAMP8)肾脏病理结构改变.方法以9月龄SAMP8为衰老动物模型,同龄抗快速老化小鼠R1(SAMR1)为对照,通过PAS、Masson染色及衰老相关β-半乳糖苷酶 (SA-β-gal) 组织化学染色观察肾脏组织病理的改变,免疫组化方法比较肾小管间质细胞外基质 (ECM)成分--纤维连接蛋白 (FN)、Ⅲ型胶原(Col Ⅲ)的变化.结果 9月龄SAMP8小鼠光镜下肾脏病理表现为小管间质纤维化、局灶节段性肾小球硬化等,以小管间质损害较为显著;SA-β-gal染色阳性细胞显著增加;免疫组化结果显示FN、ColⅢ在肾小管间质过度沉积.上述改变与SAMR1组相比,差异有显著性.结论 SAMP8肾脏的病理改变符合肾脏衰老的特点.
목적 관찰쾌속노화소서P8(SAMP8)신장병리결구개변.방법 이9월령SAMP8위쇠로동물모형,동령항쾌속노화소서R1(SAMR1)위대조,통과PAS、Masson염색급쇠로상관β-반유당감매 (SA-β-gal) 조직화학염색관찰신장조직병리적개변,면역조화방법비교신소관간질세포외기질 (ECM)성분--섬유련접단백 (FN)、Ⅲ형효원(Col Ⅲ)적변화.결과 9월령SAMP8소서광경하신장병리표현위소관간질섬유화、국조절단성신소구경화등,이소관간질손해교위현저;SA-β-gal염색양성세포현저증가;면역조화결과현시FN、ColⅢ재신소관간질과도침적.상술개변여SAMR1조상비,차이유현저성.결론 SAMP8신장적병리개변부합신장쇠로적특점.
Objective To study renal pathological structural changes in senescence accelerated mouse P8 (SAMP8). Methods SAMP8 (9 months) were used as senescence animal model and senescence accelerated mouse/resistance 1 (SAMR1) with same age as a control group. Aging kidney pathology was determined by several senescent indicators, such as PAS and Masson staining. SA-β-galactosidase activity was measured by histochemistry staining and extracellular matrix components (fibronectin and collagen Ⅲ) were measured by immunohistochemical staining. Results Compared with SAMR1 group, SAMP8 group appeared different levels of senescence. Renal pathological structural changes in SAMP8 (9 months), including tubulointerstitial fibrosis and focal segmental glomerulosclerosis, were found through microscope, and there was significant injury in the tubulointerstitial. Positive staining for SA-β-galactosidase was widely observed. And the expressions of fibronectin and collagen Ⅲ increased remarkably which indicating tubulointerstitial fibrosis. Conclusions Renal pathological changes in SAMP8 mice accord with the pathological characteristic of aging kidney.