中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2011年
11期
678-683
,共6页
张辛燕%陈晓芳%孙正%苗聪聪%葛丽华%田振川
張辛燕%陳曉芳%孫正%苗聰聰%葛麗華%田振川
장신연%진효방%손정%묘총총%갈려화%전진천
姜黄素%口腔肿瘤%动物,实验%乳香酸
薑黃素%口腔腫瘤%動物,實驗%乳香痠
강황소%구강종류%동물,실험%유향산
Curcumin%Mouth neoplasms%Animals,laboratory%Boswellic acid
目的 观察乳香酸和姜黄素对二甲基苯并蒽(7,12-dimethyl benzanthracene,DMBA)诱导的叙利亚金黄地鼠口腔癌的化学预防作用、两者单独和联合应用是否可以抑制口腔癌的发生及发展,并对其抗肿瘤机制进行初步探讨.方法 实验对象为160只6～8周龄、体质量80～130 g的雄性叙利亚金黄地鼠,取10只为阴性对照组,不作任何处理；其余150只金黄地鼠用0.5％ DMBA涂抹左侧颊囊6周,第7周时采用随机数字表法将地鼠分为5个用药组和1个阳性对照组(每组25只),低浓度乳香酸组、高浓度乳香酸组、低浓度姜黄素组、高浓度姜黄素组及联合用药组分别给予5、10 mg/L乳香酸溶液,5、10 μmol/L姜黄素溶液及5 mg/L乳香酸+5μmol/L姜黄素溶液涂抹地鼠左侧颊囊18周,阳性对照组不做任何处理.第25周处死全部动物,记录肉眼肿瘤发生率、肿瘤数目及体积,行HE、5-溴脱氧尿苷(5-bromodeoxyuridine,BrdU)、免疫组化染色及花生四烯酸代谢物检测.结果 肉眼可见肿瘤发生率阳性对照组为93.8％,低浓度乳香酸组80.0％,高浓度乳香酸组81.3％,低浓度姜黄素组78.3％,高浓度姜黄素组75.0％,联合用药组73.9％；各用药组平均肿瘤数目均较阳性对照组[(2.19±0.98)个]有所降低,其中高浓度姜黄素组[(1.45±0.92)个]和联合用药组[(1.13±0.81)个]显著下降(P＜0.05,P＜0.01)；各用药组组织病理学观察鳞状细胞癌病变数目均较阳性对照显著降低(P＜0.05).各用药组与阳性对照组相比均能降低BrdU增殖指数,尤以低、高浓度姜黄素组和联合用药组效果更佳(P＜0.05)；高、低浓度乳香酸组、高浓度姜黄素组及联合用药组白三烯B4含量与阳性对照组相比均显著下降(P＜0.05).结论 各用药组均可明显抑制DMBA诱导的金黄地鼠口腔癌,其中联合用药组抑癌作用效果最好,乳香酸和姜黄素可以通过降低细胞增殖活性抑制DMBA诱导的金黄地鼠口腔癌的发生、发展.
目的 觀察乳香痠和薑黃素對二甲基苯併蒽(7,12-dimethyl benzanthracene,DMBA)誘導的敘利亞金黃地鼠口腔癌的化學預防作用、兩者單獨和聯閤應用是否可以抑製口腔癌的髮生及髮展,併對其抗腫瘤機製進行初步探討.方法 實驗對象為160隻6～8週齡、體質量80～130 g的雄性敘利亞金黃地鼠,取10隻為陰性對照組,不作任何處理；其餘150隻金黃地鼠用0.5％ DMBA塗抹左側頰囊6週,第7週時採用隨機數字錶法將地鼠分為5箇用藥組和1箇暘性對照組(每組25隻),低濃度乳香痠組、高濃度乳香痠組、低濃度薑黃素組、高濃度薑黃素組及聯閤用藥組分彆給予5、10 mg/L乳香痠溶液,5、10 μmol/L薑黃素溶液及5 mg/L乳香痠+5μmol/L薑黃素溶液塗抹地鼠左側頰囊18週,暘性對照組不做任何處理.第25週處死全部動物,記錄肉眼腫瘤髮生率、腫瘤數目及體積,行HE、5-溴脫氧尿苷(5-bromodeoxyuridine,BrdU)、免疫組化染色及花生四烯痠代謝物檢測.結果 肉眼可見腫瘤髮生率暘性對照組為93.8％,低濃度乳香痠組80.0％,高濃度乳香痠組81.3％,低濃度薑黃素組78.3％,高濃度薑黃素組75.0％,聯閤用藥組73.9％；各用藥組平均腫瘤數目均較暘性對照組[(2.19±0.98)箇]有所降低,其中高濃度薑黃素組[(1.45±0.92)箇]和聯閤用藥組[(1.13±0.81)箇]顯著下降(P＜0.05,P＜0.01)；各用藥組組織病理學觀察鱗狀細胞癌病變數目均較暘性對照顯著降低(P＜0.05).各用藥組與暘性對照組相比均能降低BrdU增殖指數,尤以低、高濃度薑黃素組和聯閤用藥組效果更佳(P＜0.05)；高、低濃度乳香痠組、高濃度薑黃素組及聯閤用藥組白三烯B4含量與暘性對照組相比均顯著下降(P＜0.05).結論 各用藥組均可明顯抑製DMBA誘導的金黃地鼠口腔癌,其中聯閤用藥組抑癌作用效果最好,乳香痠和薑黃素可以通過降低細胞增殖活性抑製DMBA誘導的金黃地鼠口腔癌的髮生、髮展.
목적 관찰유향산화강황소대이갑기분병은(7,12-dimethyl benzanthracene,DMBA)유도적서리아금황지서구강암적화학예방작용、량자단독화연합응용시부가이억제구강암적발생급발전,병대기항종류궤제진행초보탐토.방법 실험대상위160지6～8주령、체질량80～130 g적웅성서리아금황지서,취10지위음성대조조,불작임하처리；기여150지금황지서용0.5％ DMBA도말좌측협낭6주,제7주시채용수궤수자표법장지서분위5개용약조화1개양성대조조(매조25지),저농도유향산조、고농도유향산조、저농도강황소조、고농도강황소조급연합용약조분별급여5、10 mg/L유향산용액,5、10 μmol/L강황소용액급5 mg/L유향산+5μmol/L강황소용액도말지서좌측협낭18주,양성대조조불주임하처리.제25주처사전부동물,기록육안종류발생솔、종류수목급체적,행HE、5-추탈양뇨감(5-bromodeoxyuridine,BrdU)、면역조화염색급화생사희산대사물검측.결과 육안가견종류발생솔양성대조조위93.8％,저농도유향산조80.0％,고농도유향산조81.3％,저농도강황소조78.3％,고농도강황소조75.0％,연합용약조73.9％；각용약조평균종류수목균교양성대조조[(2.19±0.98)개]유소강저,기중고농도강황소조[(1.45±0.92)개]화연합용약조[(1.13±0.81)개]현저하강(P＜0.05,P＜0.01)；각용약조조직병이학관찰린상세포암병변수목균교양성대조현저강저(P＜0.05).각용약조여양성대조조상비균능강저BrdU증식지수,우이저、고농도강황소조화연합용약조효과경가(P＜0.05)；고、저농도유향산조、고농도강황소조급연합용약조백삼희B4함량여양성대조조상비균현저하강(P＜0.05).결론 각용약조균가명현억제DMBA유도적금황지서구강암,기중연합용약조억암작용효과최호,유향산화강황소가이통과강저세포증식활성억제DMBA유도적금황지서구강암적발생、발전.
Objective To evaluate the chemopreventive effects of boswellic acid and curcumin on 7,12-dimethyl benzanthracene (DMBA)-induced oral carcinogenesis in the hamster cheek pouch model.Methods Male Syrian golden hamsters(6-8 weeks old,80-130 g in weight) were randomly divided into seven groups,with group A serving as the untreated negative control.The left cheek pouch of the remaining hamsters was topically treated with 0.5％ DMBA in mineral oil three times a week for 6 weeks.They were then randomized to six groups with group B serving as a positive control and receiving no further treatment.Groups C-G were treated topically with 5,10 mg/L boswellic acid,5,10 μ moL/L curcumin,or the combination of 5 mg/L boswellic acid and 5 μmnol/L curcumin three times per week for 18 weeks.The animals were injected with bromodeoxyuridine intraperitoneally at 50 mg/kg 2 h prior to killing.At the 25 th week all the hamsters were sacrificed and cheek pouch tissue was harvested.One half of the tissue was snap frozen in liquid nitrogen for analysis of arachidonic acid metabolites,and the other half was fixed in 10％ phosphate-buffered saline(PBS)-buffered formalin for histopathological examination.Results Six-weeks of DMBA followed by 18-weeks of topical application of boswellic acid and curcumin,both boswellic acid (5,10 mg/L) and curcumin (5,10 μmol/L) significantly inhibited the incidence from 93.8％ to 73.9％ (P＞0.05),numbers from 2.19 ± 0.98 to 1.13 ± 0.81 (P ＜ 0.01) and size of visible tumors.Microscopically the incidence of squamous cell carcinoma and BrdU index were also significantly suppressed by boswellic acid and curcumin.Conclusions Both boswellic acid and curcumin were effective in preventing oral carcinogenesis in DMBA-induced hamster cheek pouch model.
