中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2010年
1期
9-12
,共4页
李梅%李飞%张礼均%陈志%张项%单佑安%林江凯%朱刚%尹志勇%冯华
李梅%李飛%張禮均%陳誌%張項%單祐安%林江凱%硃剛%尹誌勇%馮華
리매%리비%장례균%진지%장항%단우안%림강개%주강%윤지용%풍화
脑损伤%脾切除术%死亡率%脑含水量
腦損傷%脾切除術%死亡率%腦含水量
뇌손상%비절제술%사망솔%뇌함수량
Brain injuries%Splenectomy%Mortality%Brain water content
目的 观察脾脏切除对脑创伤大鼠死亡率及脑组织含水量的影响,为提高重型颅脑创伤患者的救治水平探讨新思路.方法 成年雄性SD大鼠采用随机数字表法分为三组:颅脑创伤假手术+脾切除假手术组(A组),颅脑创伤+脾切除假手术组(B组)和颅脑创伤+脾切除组(C组).采用改进的Feeney法对大鼠右侧大脑半球进行致伤,伤后采用Longa法进行行为学评分;观察A(23只)、B(48只)、c(47只)三组大鼠致伤后7 d内的死亡率;测定各组致伤后1 d(8只)、2 d(8只)、3 d(8只)及7 d(7只)时各组大鼠脑组织含水量.结果 致伤后B、C两组之间Longs评分差异无统计学意义(P>0.05);致伤后7 d内大鼠死亡率A、B、C组分别为0%、35.4%及14.9%,各组间比较差异均有统计学意义(P<0.05);B、C组致伤侧脑含水量于伤后1,2,3及7 d时分别为(81.98±0.35)%和(81.78%±0.41)%、(82.58±0.63)%和(81.81±0.48%)(P<0.05)、(82.54±0.54)%和(81.52%±0.84)%(P<0.05),以及(81.50±0.41)%和(81.21±0.36)%.结论 颅脑创伤大鼠于伤后行脾脏切除能够降低致伤侧脑组织水肿程度,显著降低大鼠的死亡率.
目的 觀察脾髒切除對腦創傷大鼠死亡率及腦組織含水量的影響,為提高重型顱腦創傷患者的救治水平探討新思路.方法 成年雄性SD大鼠採用隨機數字錶法分為三組:顱腦創傷假手術+脾切除假手術組(A組),顱腦創傷+脾切除假手術組(B組)和顱腦創傷+脾切除組(C組).採用改進的Feeney法對大鼠右側大腦半毬進行緻傷,傷後採用Longa法進行行為學評分;觀察A(23隻)、B(48隻)、c(47隻)三組大鼠緻傷後7 d內的死亡率;測定各組緻傷後1 d(8隻)、2 d(8隻)、3 d(8隻)及7 d(7隻)時各組大鼠腦組織含水量.結果 緻傷後B、C兩組之間Longs評分差異無統計學意義(P>0.05);緻傷後7 d內大鼠死亡率A、B、C組分彆為0%、35.4%及14.9%,各組間比較差異均有統計學意義(P<0.05);B、C組緻傷側腦含水量于傷後1,2,3及7 d時分彆為(81.98±0.35)%和(81.78%±0.41)%、(82.58±0.63)%和(81.81±0.48%)(P<0.05)、(82.54±0.54)%和(81.52%±0.84)%(P<0.05),以及(81.50±0.41)%和(81.21±0.36)%.結論 顱腦創傷大鼠于傷後行脾髒切除能夠降低緻傷側腦組織水腫程度,顯著降低大鼠的死亡率.
목적 관찰비장절제대뇌창상대서사망솔급뇌조직함수량적영향,위제고중형로뇌창상환자적구치수평탐토신사로.방법 성년웅성SD대서채용수궤수자표법분위삼조:로뇌창상가수술+비절제가수술조(A조),로뇌창상+비절제가수술조(B조)화로뇌창상+비절제조(C조).채용개진적Feeney법대대서우측대뇌반구진행치상,상후채용Longa법진행행위학평분;관찰A(23지)、B(48지)、c(47지)삼조대서치상후7 d내적사망솔;측정각조치상후1 d(8지)、2 d(8지)、3 d(8지)급7 d(7지)시각조대서뇌조직함수량.결과 치상후B、C량조지간Longs평분차이무통계학의의(P>0.05);치상후7 d내대서사망솔A、B、C조분별위0%、35.4%급14.9%,각조간비교차이균유통계학의의(P<0.05);B、C조치상측뇌함수량우상후1,2,3급7 d시분별위(81.98±0.35)%화(81.78%±0.41)%、(82.58±0.63)%화(81.81±0.48%)(P<0.05)、(82.54±0.54)%화(81.52%±0.84)%(P<0.05),이급(81.50±0.41)%화(81.21±0.36)%.결론 로뇌창상대서우상후행비장절제능구강저치상측뇌조직수종정도,현저강저대서적사망솔.
Objective To observe the effect of splenectomy on mortality and brain water content of rats with brain injury so as to explore novel way for better clinical management of patients with severe traumatic brain injury. Methods Adult male SD rats were randomly divided into three groups, ie, sham operation on brain and spleen (Group A, n = 23), experimental brain trauma & sham operation on spleen (Group B, n =48) and experimental brain injury & splenectomy (Group C, n = 47). Modified Feeney' s method was used to create the animal model of experimental brain trauma, Longa' s scale was applied to evaluate the neurologic defect. Mortality within seven days following brain injury was calculat-ed. In the meantime, the brain water content was detected at days 1 (n = 8), 2 (n = 8), 3 (n = 8) and 7 (n = 7) after brain injury in each group, Results No statistical difference of Longs' s scale was found between Group B and Group C (P > 0.05). The mortalities within seven days after brain injury were 0%, 35.42 and 14.89% in Groups A, B and C respectively, with statistical difference between groups (P<0.05). The brain water content of Groups B and C at days 1, 2, 3 and 7 were (81.98±0.35)% & (81.78±0.41)%, (82.58±0.63)% & (81.81±0.48)% (P<0.05),(82.54±0.54)% & (81.52±0.84)% (P<0.05) and (81.50±0.41)% & (81.21±0.36)% (P>0.05) respectively. Conclusion Splenectomy can effectively reduce brain water content and significantly decrease mortality in rata with brain injury.
