中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2009年
3期
237-241
,共5页
傅窈窈%张天宇%赵晖%李雯
傅窈窈%張天宇%趙暉%李雯
부요요%장천우%조휘%리문
地塞米松%受体,糖皮质激素%耳蜗%组织分布%大鼠
地塞米鬆%受體,糖皮質激素%耳蝸%組織分佈%大鼠
지새미송%수체,당피질격소%이와%조직분포%대서
Dexamethasene%Receptors,glucocorticoid%Cochlea%Tissue distribution%Rats
目的 观察不同浓度地塞米松鼓室内注射后在大鼠耳蜗中的分布及代谢规律.方法 144只SD大鼠全麻下鼓室内分别注射5 mg/ml、10 mg/ml和20 mg/ml地塞米松磷酸钠,并分别于注射后5 min、10 min、15 min、30 min、1 h、2 h,4 h,8 h、12 h、24 h、48 h及72 h处死动物,每个浓度、每个时间点4只大鼠.耳蜗标本处理后冰冻切片,免疫荧光法观察地塞米松在耳蜗组织中随时间的变化情况,并进行荧光半定量分析.另取4只正常sD大鼠采用免疫荧光方法 观察糖皮质激素受体在耳蜗内的分布.结果 地塞米松自注射后15 min起在耳蜗内始被检测到,30 min达到高峰,主要分布在螺旋韧带,Corti器和螺旋神经节.10 mg/ml组与20 mg/ml组各时间点的地塞米松浓度均高于5mg/ml组,持续时间亦从低浓度组的48 h延长至72 h.糖皮质激素受体亦主要分布于Corti器,螺旋神经节和螺旋韧带.结论 地塞米松鼓室内注射后可迅速到达耳蜗组织细胞中,其分布与糖皮质激素受体基本一致.增加地塞米松浓度可以提高其在耳蜗组织中的分布浓度,并延长其存留时间.
目的 觀察不同濃度地塞米鬆鼓室內註射後在大鼠耳蝸中的分佈及代謝規律.方法 144隻SD大鼠全痳下鼓室內分彆註射5 mg/ml、10 mg/ml和20 mg/ml地塞米鬆燐痠鈉,併分彆于註射後5 min、10 min、15 min、30 min、1 h、2 h,4 h,8 h、12 h、24 h、48 h及72 h處死動物,每箇濃度、每箇時間點4隻大鼠.耳蝸標本處理後冰凍切片,免疫熒光法觀察地塞米鬆在耳蝸組織中隨時間的變化情況,併進行熒光半定量分析.另取4隻正常sD大鼠採用免疫熒光方法 觀察糖皮質激素受體在耳蝸內的分佈.結果 地塞米鬆自註射後15 min起在耳蝸內始被檢測到,30 min達到高峰,主要分佈在螺鏇韌帶,Corti器和螺鏇神經節.10 mg/ml組與20 mg/ml組各時間點的地塞米鬆濃度均高于5mg/ml組,持續時間亦從低濃度組的48 h延長至72 h.糖皮質激素受體亦主要分佈于Corti器,螺鏇神經節和螺鏇韌帶.結論 地塞米鬆鼓室內註射後可迅速到達耳蝸組織細胞中,其分佈與糖皮質激素受體基本一緻.增加地塞米鬆濃度可以提高其在耳蝸組織中的分佈濃度,併延長其存留時間.
목적 관찰불동농도지새미송고실내주사후재대서이와중적분포급대사규률.방법 144지SD대서전마하고실내분별주사5 mg/ml、10 mg/ml화20 mg/ml지새미송린산납,병분별우주사후5 min、10 min、15 min、30 min、1 h、2 h,4 h,8 h、12 h、24 h、48 h급72 h처사동물,매개농도、매개시간점4지대서.이와표본처리후빙동절편,면역형광법관찰지새미송재이와조직중수시간적변화정황,병진행형광반정량분석.령취4지정상sD대서채용면역형광방법 관찰당피질격소수체재이와내적분포.결과 지새미송자주사후15 min기재이와내시피검측도,30 min체도고봉,주요분포재라선인대,Corti기화라선신경절.10 mg/ml조여20 mg/ml조각시간점적지새미송농도균고우5mg/ml조,지속시간역종저농도조적48 h연장지72 h.당피질격소수체역주요분포우Corti기,라선신경절화라선인대.결론 지새미송고실내주사후가신속도체이와조직세포중,기분포여당피질격소수체기본일치.증가지새미송농도가이제고기재이와조직중적분포농도,병연장기존류시간.
Objective To investigate the distribution and pharmacokinetics of dexamethasone of different concentrations in the inner ears of SD rats after intratympanic injection. Methods Totally 144 adult SD rats were anaesthetized and dexamethasone sodium phosphate of different concentrations(5 mg/ml, 10 mg/ml, 20 mg/ml) was injected into the tympanums. The rats were sacrificed at various postinjection survival times (5 min, 10 min, 15 min, 30 min, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h, 48 h, 72 h) ,and every 4 rats were included into each group. Then after a series of processes the inner ear tissue was cryostat sectioned. The distribution of dexamethasone was evaluated using immunofluorescence with semiquantitative analysis. Immunofluorescenee was also used in another 4 normal SD rats to detect the distribution of Glucocorticoid receptor (GR) in the inner ear. Results Dexamethasone was observed initially 15 min after local drug administration and 30 min to its peak level. The highest concentration of dexamethasone labeling was seen in the spiral ligament, organ of Corti and spiral ganglion, which paralleled the distribution of GR. The tissue concentration of 10 mg/ml and 20 mg/ml groups was higher than 5 mg/ml every corresponding time point, and the lasting time was also prolonged from 48 hours to 72 hours. Conclusions Dexamethasone can enter into the cochlear tissue quickly after transtympanic injection, and its distribution accords nearly exactly with that of GR. Increase of the concentration of dexamethasone results in higher tissue distribution and longer lasting time.
