CAJ | 학술논문

目的评价椎间孔注射多柔比星对神经病理性痛大鼠痛行为学和背根神经节(DRG)神经元超微结构的影响.方法健康雄性SD大鼠100只,体重200～250 g,3月龄,采用坐骨神经分支选择性损伤(SNI)的方法建立大鼠神经病理性痛模型,采用随机数字表法,将大鼠随机分为5组(n=20):SNI组和不同浓度多柔比星组(A1组、A2组、A3组和A4组),于模型建立2周后,L4,5和L5,6椎间孔分别注射生理盐水、0.25％、0.50％、0.75％和1.00％多柔比星5 μl.于给药前及给药后1～28 d测定热辐射缩足潜伏期及冷刺激缩足持续时间,于给药后28 d取3只大鼠,取DRG,电镜下观察大、小神经元超微结构.结果与SNI组比较,A1组、A2组、A3组及A4组热辐射缩足潜伏期延长,冷刺激缩足持续时间缩短,呈浓度依赖性(P＜0.05).电镜下SNI组DRG神经元超微结构未见异常,A1组、A2组、A3组和A4组DRG小神经元细胞核固缩、线粒体肿胀、溶酶体增多、染色质边集、细胞间质纤维化和细胞坏死,而大神经元线粒体肿胀及纤维增多,多数大神经元存活.结论椎间孔注射多柔比星可减轻大鼠神经病理性痛,可导致DRG大、小神经元损伤,小神经元的损伤更明显.
목적 평개추간공주사다유비성대신경병이성통대서통행위학화배근신경절(DRG)신경원초미결구적영향.방법 건강웅성SD대서100지,체중200～250 g,3월령,채용좌골신경분지선택성손상(SNI)적방법건립대서신경병이성통모형,채용수궤수자표법,장대서수궤분위5조(n=20):SNI조화불동농도다유비성조(A1조、A2조、A3조화A4조),우모형건립2주후,L4,5화L5,6추간공분별주사생리염수、0.25％、0.50％、0.75％화1.00％다유비성5 μl.우급약전급급약후1～28 d측정열복사축족잠복기급랭자격축족지속시간,우급약후28 d취3지대서,취DRG,전경하관찰대、소신경원초미결구.결과 여SNI조비교,A1조、A2조、A3조급A4조열복사축족잠복기연장,랭자격축족지속시간축단,정농도의뢰성(P＜0.05).전경하SNI조DRG신경원초미결구미견이상,A1조、A2조、A3조화A4조DRG소신경원세포핵고축、선립체종창、용매체증다、염색질변집、세포간질섬유화화세포배사,이대신경원선립체종창급섬유증다,다수대신경원존활.결론 추간공주사다유비성가감경대서신경병이성통,가도치DRG대、소신경원손상,소신경원적손상경명현.
Objective To investigate the effects of paravertebral injection of doxorubicin on pain behavior and ultrastructure of dorsal root ganglion ( DRG ) neurons in rats with neuropathic pain.Methods One hundred male SD rats,aged 3 months,weighing 200-250 g,were studied.The spared nerve injury (SNI) model was established by ligation and separation of tibial and common fibular nerves.The rats were randomly divided into 5 groups ( n =20 each):SNI group and different concentrations of doxorubicin groups (groups A1,A2,A3,A4 ).The animals were anesthetized with intrapetitaneal 10％ chioral hydrate 0.3 ml/100 g.The L4,6 intervertebral foramina was exposed.Normal saline and 0.25％,0.50％,0.75％ and 1.00％ doxorubicin 5 μl were injected into the intervertebral foramina in groups SNI,A1,A2,A3,and A4 respectively.The paw withdrawal latency to the radiant heat stimulation (PWL) and paw withdrawal duration to acetone (PWD) were measured before administration and on day 1-28 after administration.Three rats were chosen in each group on day 28 after administration and DRG were removed to observe the ultrastructure of large and small neurons with electron microscope.Results Compared with group SNI,PWL was significantly prolonged and PWD was significantly shortened in groups A1,A2,A3,A4 in a concentration manner ( P ＜ 0.05).Nucleus condensation,mitochondrial swelling,increased lysosomes,chromatin condensation,fibrosis of intercellular substances,and cell necrosis were observed in small neurons.Mitochondrial swelling and increased fibers wcre observed in large neurons and most of large neurons survived.Conclusion Paravertebral injection of doxorubicin can reduce neuropathic pain and result in damage to large and small DRG neurons,and small neurons in the DRG are more susceptible and vulnerable to doxorubicin than large neurons in rats.