CAJ | 학술논문

目的研究2008年青海省手足口病(Hand,Foot and Mouth Disease,HFMD)的致病病原体中肠道病毒71型(Human Enterovirus 71,HEV71)的VP1区基因特征.方法采集青海省HFMD患者的粪便、疱疹液和咽拭子标本共335份,并进行病毒分离,然后对阳性分离株病毒进行肠道病毒血清型别的分子定型.对分离到的HEV71阳性分离株进行VP1编码区基因扩增,核苷酸序列测定和同源进化分析.结果在335份临床标本中,共分离到45株阳性病毒分离物,其中30株鉴定为HEV71(占66.7%),是主要病原体.对30株HEV71进行VP1区核苷酸序列测定后,分析发现它们在VP1区核苷酸水平和氨基酸水平上有一定差异,同源性分别在95.2%～100.0%和96.6%～100%之间.它们与1998年以来在我国分离的HEV71在核苷酸和氨基酸水平上的同源性都较高.与其他35株各基因型和基因亚型的HEV71代表株构建的亲缘进化树中显示,青海省HEY71分离株与C4亚型代表株聚为一簇,属于C4基因亚型C4a进化分支.结论青海省从HFMD病例中分离到HEV71,也确认了青海省HFMD的病原体HEV71流行株的基因型为C4基因型中的C4a进化分支,并且存在多个传播链.
목적 연구2008년청해성수족구병(Hand,Foot and Mouth Disease,HFMD)적치병병원체중장도병독71형(Human Enterovirus 71,HEV71)적VP1구기인특정.방법 채집청해성HFMD환자적분편、포진액화인식자표본공335빈,병진행병독분리,연후대양성분리주병독진행장도병독혈청형별적분자정형.대분리도적HEV71양성분리주진행VP1편마구기인확증,핵감산서렬측정화동원진화분석.결과 재335빈림상표본중,공분리도45주양성병독분리물,기중30주감정위HEV71(점66.7%),시주요병원체.대30주HEV71진행VP1구핵감산서렬측정후,분석발현타문재VP1구핵감산수평화안기산수평상유일정차이,동원성분별재95.2%～100.0%화96.6%～100%지간.타문여1998년이래재아국분리적HEV71재핵감산화안기산수평상적동원성도교고.여기타35주각기인형화기인아형적HEV71대표주구건적친연진화수중현시,청해성HEY71분리주여C4아형대표주취위일족,속우C4기인아형C4a진화분지.결론 청해성종HFMD병례중분리도HEV71,야학인료청해성HFMD적병원체HEV71류행주적기인형위C4기인형중적C4a진화분지,병차존재다개전파련.
Objective To study the genetic characterizations of VP1 gene of human enterovirus 71 ( HEV71 ) isolated from clinical specimens of Hand, Foot and Mouth Disease (HFMD) patients in Qinghai Province in 2008. Methods 335 clinical samples including stools, throat swabs and vesicle fluids were collected from HFMD patients in Qinghai Province. Viral isolation was performed, and molecular typing was performed with the positive isolates. Then 30 identified HEV71 isolates were performed for entire VP1 coding region amplification and sequencing. Results Among the 355 clinical samples, 45 human enteroviruses were isolated, and among them, 30 were identified as HEV71. Then 30 HEV71 positive isolates were performed by nucleotide sequencing. It showed that there was some difference in the nucleotide and the amino acid among the 30 HEV71 strains, the homology were 95.2%-100% and 96.6%-100%,respectively. But they all closed to HEV71 strains isolated in China after 1998, and from the phylogenetic tree constructed with 30 Qinghai HEV71 strains and other 35 HEV71 strains represented all known genotype and subgenotype HEV71 strains available from GenBank, it revealed that the 30 Qinghai HEV71 strains clustered within the C4a evolution branch of C4 subgenotype. Conclusion HEV71 was isolated in HFMD patients in Qinghai province, and the HEV71 strains causing HFMD outbreaks in Qinghai province in 2008 were all belong to C4a evolution branch of C4 subgenotype with several transmission chains.