中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2010年
9期
815-819
,共5页
张素萍%吴昭昭%武彦文%苏世标%童建
張素萍%吳昭昭%武彥文%囌世標%童建
장소평%오소소%무언문%소세표%동건
本底辐射%疾病影响状态调查%适应性反应%S100 蛋白质类%糖基化终产物%高级
本底輻射%疾病影響狀態調查%適應性反應%S100 蛋白質類%糖基化終產物%高級
본저복사%질병영향상태조사%괄응성반응%S100 단백질류%당기화종산물%고급
Background radiation%Sickness impact profile%Adaptive response%S100 proteins%Glycosylation end products,advanced
目的 通过检测广东阳江天然高本底辐射地区(high background radiation area,HBRA)和对照低辐射地区(control area,CA)居民晚期糖基化终产物受体(receptor for advanced glycation endproducts,RAGE)和钙结合蛋白S100A6 mRNA及蛋白的表达,研究HBRA居民适应性反应机制.方法 以HBRA及CA居民为研究对象,分别选取50～60岁健康无遗传疾病家族史的男性长住居民各53名,收集血液及痰液样品,逆转录PCR(RT-PCR)检测血细胞中RAGE和S100A6 mRNA的表达,Western Blotting方法 分析痰细胞中RAGE和S100A6蛋白的表达.热释光剂量计(thermo luminescent dosemeter,TLD)检测居民暴露水平的外照射剂量及评估年有效剂量.结果 CA和HBRA组的平均年有效剂量分别为1.95 mSv和6.24 mSv,HBRA组的剂量约为CA组的3倍.与CA组相比,HBRA组居民RAGE与S100A6 mRNA与蛋白的表达量均有明显下降:以β-肌动蛋白(β-actin)为内参,血细胞mRNA中,RAGE和S100A6在CA组的相对中位表达量分别为0.28和1.06;而在HBRA组中分别为0.16和0.79,以Wilcoxon秩和检验分析,两者差异有统计学意义(Z值分别为-2.587和-2.328,P<0.05).痰细胞总蛋白中,以β-actin为内参,RAGE和S100A6在CA组的相对中位表达量分别为2.98和2.25;而在HBRA组中分别为0.53和0.47,以Wilcoxon秩和检验分析,两者差异有统计学意义(Z值分别为-2.201和-2.366,P<0.05).结论 长期接受天然高本底辐射的人群中RAGE及S100A6的表达量降低,可能与HBRA居民的适应性反应及低癌症死亡率有关.
目的 通過檢測廣東暘江天然高本底輻射地區(high background radiation area,HBRA)和對照低輻射地區(control area,CA)居民晚期糖基化終產物受體(receptor for advanced glycation endproducts,RAGE)和鈣結閤蛋白S100A6 mRNA及蛋白的錶達,研究HBRA居民適應性反應機製.方法 以HBRA及CA居民為研究對象,分彆選取50～60歲健康無遺傳疾病傢族史的男性長住居民各53名,收集血液及痰液樣品,逆轉錄PCR(RT-PCR)檢測血細胞中RAGE和S100A6 mRNA的錶達,Western Blotting方法 分析痰細胞中RAGE和S100A6蛋白的錶達.熱釋光劑量計(thermo luminescent dosemeter,TLD)檢測居民暴露水平的外照射劑量及評估年有效劑量.結果 CA和HBRA組的平均年有效劑量分彆為1.95 mSv和6.24 mSv,HBRA組的劑量約為CA組的3倍.與CA組相比,HBRA組居民RAGE與S100A6 mRNA與蛋白的錶達量均有明顯下降:以β-肌動蛋白(β-actin)為內參,血細胞mRNA中,RAGE和S100A6在CA組的相對中位錶達量分彆為0.28和1.06;而在HBRA組中分彆為0.16和0.79,以Wilcoxon秩和檢驗分析,兩者差異有統計學意義(Z值分彆為-2.587和-2.328,P<0.05).痰細胞總蛋白中,以β-actin為內參,RAGE和S100A6在CA組的相對中位錶達量分彆為2.98和2.25;而在HBRA組中分彆為0.53和0.47,以Wilcoxon秩和檢驗分析,兩者差異有統計學意義(Z值分彆為-2.201和-2.366,P<0.05).結論 長期接受天然高本底輻射的人群中RAGE及S100A6的錶達量降低,可能與HBRA居民的適應性反應及低癌癥死亡率有關.
목적 통과검측엄동양강천연고본저복사지구(high background radiation area,HBRA)화대조저복사지구(control area,CA)거민만기당기화종산물수체(receptor for advanced glycation endproducts,RAGE)화개결합단백S100A6 mRNA급단백적표체,연구HBRA거민괄응성반응궤제.방법 이HBRA급CA거민위연구대상,분별선취50～60세건강무유전질병가족사적남성장주거민각53명,수집혈액급담액양품,역전록PCR(RT-PCR)검측혈세포중RAGE화S100A6 mRNA적표체,Western Blotting방법 분석담세포중RAGE화S100A6단백적표체.열석광제량계(thermo luminescent dosemeter,TLD)검측거민폭로수평적외조사제량급평고년유효제량.결과 CA화HBRA조적평균년유효제량분별위1.95 mSv화6.24 mSv,HBRA조적제량약위CA조적3배.여CA조상비,HBRA조거민RAGE여S100A6 mRNA여단백적표체량균유명현하강:이β-기동단백(β-actin)위내삼,혈세포mRNA중,RAGE화S100A6재CA조적상대중위표체량분별위0.28화1.06;이재HBRA조중분별위0.16화0.79,이Wilcoxon질화검험분석,량자차이유통계학의의(Z치분별위-2.587화-2.328,P<0.05).담세포총단백중,이β-actin위내삼,RAGE화S100A6재CA조적상대중위표체량분별위2.98화2.25;이재HBRA조중분별위0.53화0.47,이Wilcoxon질화검험분석,량자차이유통계학의의(Z치분별위-2.201화-2.366,P<0.05).결론 장기접수천연고본저복사적인군중RAGE급S100A6적표체량강저,가능여HBRA거민적괄응성반응급저암증사망솔유관.
Objective To study the adaptive response mechanisms in high background radiation area(HBRA) among Yangjiang local people through gene and protein expression of receptor for advanced glycation endproducts (RAGE) and S100A6 in peripheral blood and sputum in inhabitants of HBRA. Methods A total of 53 male inhabitants were selected from HBRA in Yangjiang as the exposure group, while 53 male inhabitants were selected from Enping (control area, CA)as the control group. The content of RAGE and S100A6 gene and protein were detected by RT-PCR and Western blotting assay. Thermo luminescent dosemeter(TLD) assay was used to measure the outside dose and estimate the effective dose. Results The effective dose in CA and HBRA was respectively 1.95 mSv and 6. 24 mSv,which was 3 fold difference. Compared with CA, RAGE and S100A6 expression were significantly reduced in both gene and protein level in HBRA. The relative median mRNA expression of RAGE and S100A6 in peripheral blood were respectively 0. 28,1.06 and 0. 16,0. 79 in CA and HBRA group,there was significance (with analysis Z values of - 2. 587 and - 2. 328 respectively,P < 0. 05) with Wilcoxon rank test. For the protein of sputum, the relative median expression were respectively 2. 98, 2. 25 and 0. 53,0. 47 with significant difference (with analysis Z values of - 2. 201 and - 2. 366 respectively, P < 0. 05) by Wilcoxon rank test. Conclusion The low expression of RAGE and S100A6 in HBRA group might be correlated with the adaptive response and the low mortality of cancer in HBRA.
