CAJ | 학술논문

为了探讨藜叶中黄酮类化合物的体外抗氧化活性,采用有机溶剂提取法和色谱柱法对藜叶中化学成分进行提取与分离;以Vc作对照,对分离纯化的芦丁、乙酸乙酯浸膏和正丁醇浸膏进行DPPH·、O_2~-·和·OH的清除效果试验.结果表明:三者对DPPH·、O_2~-·、·OH均具有清除作用,且与浓度呈量效关系,芦丁对·OH清除效果优于V_C,芦丁具有较强的清除DPPH·能力,其IC_(50)为0.05 μg/mL.
위료탐토려협중황동류화합물적체외항양화활성,채용유궤용제제취법화색보주법대려협중화학성분진행제취여분리;이Vc작대조,대분리순화적호정、을산을지침고화정정순침고진행DPPH·、O_2~-·화·OH적청제효과시험.결과표명:삼자대DPPH·、O_2~-·、·OH균구유청제작용,차여농도정량효관계,호정대·OH청제효과우우V_C,호정구유교강적청제DPPH·능력,기IC_(50)위0.05 μg/mL.
In order to discuss antioxidant activity in vitro of flavonoids from Chenopodium album L. leaves, with solvent extraction method and chromatogram column method, rutin is acquired from Chenopodium album L. leaves; The free radi-cal scavenging activities of rutin,ethyl acetate part and n-butanol part was studied in superoxide anion(O_2~-·) ,hydroxyl radical(·OH) and DPPH · systems ,which is compared with Vc. The results showed the three samples have scavenging capability against DPPH ·, O_2~-·and ·OH radicals in a dose dependent manner, rutin is stronger than V_C for scaven-ging · OH radical, rutin has prominent effect of scavenging DPPH ·radical, IC_(50) is 0.05μg/mL.