中国老年学杂志
中國老年學雜誌
중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2010年
3期
345-347
,共3页
基质金属蛋白酶-9%RNA干扰%慢病毒载体
基質金屬蛋白酶-9%RNA榦擾%慢病毒載體
기질금속단백매-9%RNA간우%만병독재체
Matrix metalloproteinase (MMP)-9%RNA interference%Lentivirus vector
目的 探讨构建的小鼠基质金属蛋白酶(MMP)-9 RNA干扰(RNAi)慢病毒载体在体外工具细胞中的沉默效应.方法 针对小鼠MMP-9基因序列构建的MMP-9过表达克隆质粒和RNAi慢病毒载体共转染培养良好的工具细胞(293T和NIH3T3细胞),采用实时荧光定量PCR和Western印迹观察RNAi对工具细胞中MMP-9 mRNA和蛋白表达的下调作用.结果 构建的MMP-9 RNAi慢病毒载体能显著降低工具细胞中MMP-9表达量.结论 成功构建了能够抑制小鼠MMP-9表达的RNAi慢病毒载体,为在体研究奠定了基础.
目的 探討構建的小鼠基質金屬蛋白酶(MMP)-9 RNA榦擾(RNAi)慢病毒載體在體外工具細胞中的沉默效應.方法 針對小鼠MMP-9基因序列構建的MMP-9過錶達剋隆質粒和RNAi慢病毒載體共轉染培養良好的工具細胞(293T和NIH3T3細胞),採用實時熒光定量PCR和Western印跡觀察RNAi對工具細胞中MMP-9 mRNA和蛋白錶達的下調作用.結果 構建的MMP-9 RNAi慢病毒載體能顯著降低工具細胞中MMP-9錶達量.結論 成功構建瞭能夠抑製小鼠MMP-9錶達的RNAi慢病毒載體,為在體研究奠定瞭基礎.
목적 탐토구건적소서기질금속단백매(MMP)-9 RNA간우(RNAi)만병독재체재체외공구세포중적침묵효응.방법 침대소서MMP-9기인서렬구건적MMP-9과표체극륭질립화RNAi만병독재체공전염배양량호적공구세포(293T화NIH3T3세포),채용실시형광정량PCR화Western인적관찰RNAi대공구세포중MMP-9 mRNA화단백표체적하조작용.결과 구건적MMP-9 RNAi만병독재체능현저강저공구세포중MMP-9표체량.결론 성공구건료능구억제소서MMP-9표체적RNAi만병독재체,위재체연구전정료기출.
Objective To explore the silencing effect of the constructed mice's matrix metalloproteinase (MMP)-9 RNA interference (RNAi) lentivirus vector in vitro. Methods 293T and NIH3T3 cells were cocultured by MMP-9 overexpressed clone plasmid and RNAi lentivirus vector. Real time PCR and Western blot were adopted to observe the downregulation of MMP-9 mRNA expression in 293T and NIH3T3 cells by RNAi. Results The constructed MMP-9 RNAi lentivirus vector significantly decreased MMP-9 expression in 293T and NIH3T3 cells. Conclusions RNAi lentivirus vector inhibiting MMP-9 expression is successfully constructed, laying foundation for the research in vivo.
