蚕业科学
蠶業科學
잠업과학
ACTA SERICOLOGICA SINICA
2010年
1期
109-114
,共6页
娄德帅%范涛%王储炎%颜辉%桂仲争
婁德帥%範濤%王儲炎%顏輝%桂仲爭
루덕수%범도%왕저염%안휘%계중쟁
全蚕粉%1-脱氧野尻霉素%低温冻干%阳离子交换树脂分离%抗肿瘤
全蠶粉%1-脫氧野尻黴素%低溫凍榦%暘離子交換樹脂分離%抗腫瘤
전잠분%1-탈양야고매소%저온동간%양리자교환수지분리%항종류
Silkworm powder%Deoxynojirimycin (DNJ)%Freeze drying%Isolation with cation exchange resin%Anti-tumor
利用柱前衍生化反相高效液相色谱法检测不同前处理全蚕粉中1-脱氧野尻霉素(1-deoxynojirimycin, DNJ)的提取分离效果,并研究其分离产物对人宫颈癌HeLa细胞的抗肿瘤作用.结果表明,超低温冷冻干燥、热风烘干和真空浓缩煎煮冷冻干燥所得全蚕粉中DNJ的质量分数分别为 0.494 9%、0.383 9%和0.607 6%;冷冻干燥全蚕粉粉碎粒度为0.15、0.25和0.425 mm时,DNJ质量分数分别为0.494 9%、0.321 5%和0.089 5%;用稀酸浸渍及阳离子交换树脂提取与分离全蚕粉中的DNJ效果最佳,分离物中DNJ的质量分数达到1.08%.浓度为 40 mg/mL的全蚕粉分离物对肿瘤细胞的生长抑制率达92.27%,可使肿瘤细胞增殖能力完全丧失.结果提示:采用真空浓缩煎煮结合超低温冷冻干燥、超细粉碎和稀酸浸渍及阳离子交换树脂分离技术,可有效提高全蚕粉中DNJ的提取、分离效率,含DNJ的全蚕粉分离物对HeLa肿瘤细胞的生长具有显著的抑制作用.
利用柱前衍生化反相高效液相色譜法檢測不同前處理全蠶粉中1-脫氧野尻黴素(1-deoxynojirimycin, DNJ)的提取分離效果,併研究其分離產物對人宮頸癌HeLa細胞的抗腫瘤作用.結果錶明,超低溫冷凍榦燥、熱風烘榦和真空濃縮煎煮冷凍榦燥所得全蠶粉中DNJ的質量分數分彆為 0.494 9%、0.383 9%和0.607 6%;冷凍榦燥全蠶粉粉碎粒度為0.15、0.25和0.425 mm時,DNJ質量分數分彆為0.494 9%、0.321 5%和0.089 5%;用稀痠浸漬及暘離子交換樹脂提取與分離全蠶粉中的DNJ效果最佳,分離物中DNJ的質量分數達到1.08%.濃度為 40 mg/mL的全蠶粉分離物對腫瘤細胞的生長抑製率達92.27%,可使腫瘤細胞增殖能力完全喪失.結果提示:採用真空濃縮煎煮結閤超低溫冷凍榦燥、超細粉碎和稀痠浸漬及暘離子交換樹脂分離技術,可有效提高全蠶粉中DNJ的提取、分離效率,含DNJ的全蠶粉分離物對HeLa腫瘤細胞的生長具有顯著的抑製作用.
이용주전연생화반상고효액상색보법검측불동전처리전잠분중1-탈양야고매소(1-deoxynojirimycin, DNJ)적제취분리효과,병연구기분리산물대인궁경암HeLa세포적항종류작용.결과표명,초저온냉동간조、열풍홍간화진공농축전자냉동간조소득전잠분중DNJ적질량분수분별위 0.494 9%、0.383 9%화0.607 6%;냉동간조전잠분분쇄립도위0.15、0.25화0.425 mm시,DNJ질량분수분별위0.494 9%、0.321 5%화0.089 5%;용희산침지급양리자교환수지제취여분리전잠분중적DNJ효과최가,분리물중DNJ적질량분수체도1.08%.농도위 40 mg/mL적전잠분분리물대종류세포적생장억제솔체92.27%,가사종류세포증식능력완전상실.결과제시:채용진공농축전자결합초저온냉동간조、초세분쇄화희산침지급양리자교환수지분리기술,가유효제고전잠분중DNJ적제취、분리효솔,함DNJ적전잠분분리물대HeLa종류세포적생장구유현저적억제작용.
By using the pre-column derivatization reverse phase high performance liquid chromatography, the efficiency of 1-deoxynojirimycin (DNJ) isolation from silkworm powder prepared with different pre-treatment methods was investigated. The obtained isolate was examined for their inhibitory effect to HeLa tumor cells. The results showed that, in the ultra-low temperature freeze-dried, heat-dried and vacuum condensation and decoction combining with ultra-low temperature freeze-dried, silkworm powder samples, DNJ content was 0.494 9%, 0.383 9% and 0.607 6% respectively. DNJ content in the freeze-dried samples with particle sizes of 0.15, 0.25 and 0.425 mm was 0.494 9%, 0.321 5% and 0.089 5%, respectively. In the isolate obtained from dilute acid immersion and cation exchange resin, DNJ content was as high as 1.08%. The silkworm powder isolate with concentration of 40 mg/mL had inhibition efficiency of 92.27% to HeLa tumor cells, being able to eliminate tumor cell proliferation completely. The results implied that, vacuum condensation and decoction combining with ultra-low temperature freeze-drying, ultra-fine grinding technology and dilute acid immersion and cation exchange resin could improve the isolation efficiency of DNJ from silkworm powder remarkably, and the silkworm powder isolate containing DNJ had significant inhibitory effect to the growth of HeLa tumor cells.
