国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2009年
2期
100-102
,共3页
单平因%卢志勇%宣国红%谈素红%陈海潮%茹金城%许德顺
單平因%盧誌勇%宣國紅%談素紅%陳海潮%茹金城%許德順
단평인%로지용%선국홍%담소홍%진해조%여금성%허덕순
肝炎,乙型%表面大蛋白%病毒复制
肝炎,乙型%錶麵大蛋白%病毒複製
간염,을형%표면대단백%병독복제
. Hepatitis B%Large surface protein%Virus replication
目的 检测乙型肝炎患者血清中3种HBV标志物(HBV-M)模式的HBV表面大蛋白(LHBs)和HBV DNA,对比并观察其相关关系,探讨HBeAg阴性的乙型肝炎检测LHBs的临床价值.方法 对534例乙型肝炎患者中的HBeAg阴性者346例,以ELISA法进行LHBs和HBV-M检测,荧光定量PCR方法 进行HBV DNA检测,并与HBeAg阳性者的检测结果 作比较.结果 534份乙型肝炎患者的LHBs、HBV DNA检测结果 显示:L组(大三阳)LHBs与HBV DNA检测阳性率分别为95.21%和86.17%,两种检测方法 之间有很好的相关性;S组(小三阳)及DN组(HBeAg和抗-Hbe均阴性)LHBs与HBV DNA阳性率分别为73.67%、81.48%和42.32%、55.56%,差异均有统计学意义(P<0.01,P<0.05);HBeAg阴性的346例样本中LHBs与HBV DNA的阳性率分别为71.39%和43.35%,差异有统计学意义(P<0.01),其中HBV DNA检测的196例阴性样本经LHBs检测阳性率为68.88%.结论 LHBs可作为从蛋白水平反映乙型肝炎患者体内病毒复制的血清学指标,且与HBV DNA检测具有很好的相关性,特别对于HBeAg阴性的患者LHBs检测可作为体内HBV复制及预后判断的较好血清免疫学指标.
目的 檢測乙型肝炎患者血清中3種HBV標誌物(HBV-M)模式的HBV錶麵大蛋白(LHBs)和HBV DNA,對比併觀察其相關關繫,探討HBeAg陰性的乙型肝炎檢測LHBs的臨床價值.方法 對534例乙型肝炎患者中的HBeAg陰性者346例,以ELISA法進行LHBs和HBV-M檢測,熒光定量PCR方法 進行HBV DNA檢測,併與HBeAg暘性者的檢測結果 作比較.結果 534份乙型肝炎患者的LHBs、HBV DNA檢測結果 顯示:L組(大三暘)LHBs與HBV DNA檢測暘性率分彆為95.21%和86.17%,兩種檢測方法 之間有很好的相關性;S組(小三暘)及DN組(HBeAg和抗-Hbe均陰性)LHBs與HBV DNA暘性率分彆為73.67%、81.48%和42.32%、55.56%,差異均有統計學意義(P<0.01,P<0.05);HBeAg陰性的346例樣本中LHBs與HBV DNA的暘性率分彆為71.39%和43.35%,差異有統計學意義(P<0.01),其中HBV DNA檢測的196例陰性樣本經LHBs檢測暘性率為68.88%.結論 LHBs可作為從蛋白水平反映乙型肝炎患者體內病毒複製的血清學指標,且與HBV DNA檢測具有很好的相關性,特彆對于HBeAg陰性的患者LHBs檢測可作為體內HBV複製及預後判斷的較好血清免疫學指標.
목적 검측을형간염환자혈청중3충HBV표지물(HBV-M)모식적HBV표면대단백(LHBs)화HBV DNA,대비병관찰기상관관계,탐토HBeAg음성적을형간염검측LHBs적림상개치.방법 대534례을형간염환자중적HBeAg음성자346례,이ELISA법진행LHBs화HBV-M검측,형광정량PCR방법 진행HBV DNA검측,병여HBeAg양성자적검측결과 작비교.결과 534빈을형간염환자적LHBs、HBV DNA검측결과 현시:L조(대삼양)LHBs여HBV DNA검측양성솔분별위95.21%화86.17%,량충검측방법 지간유흔호적상관성;S조(소삼양)급DN조(HBeAg화항-Hbe균음성)LHBs여HBV DNA양성솔분별위73.67%、81.48%화42.32%、55.56%,차이균유통계학의의(P<0.01,P<0.05);HBeAg음성적346례양본중LHBs여HBV DNA적양성솔분별위71.39%화43.35%,차이유통계학의의(P<0.01),기중HBV DNA검측적196례음성양본경LHBs검측양성솔위68.88%.결론 LHBs가작위종단백수평반영을형간염환자체내병독복제적혈청학지표,차여HBV DNA검측구유흔호적상관성,특별대우HBeAg음성적환자LHBs검측가작위체내HBV복제급예후판단적교호혈청면역학지표.
Objective To detect large surface protein (LHBs)and HBV DNA on 3 HBV-M and compare their difference, to study the relation between LHBs and HBV DNA and to explore the clinical value of detecting LHBs among HBeAg negative in the patients with hepatitis B. Methods 346 HBeAg negative patients among 534 cases were detected LHBs and HBV DNA respectively. LHBs were measured by ELISA,while serum HBV DNA level by fluorescent quantitative PCR, and the results were also compared to those observed form HBeAg positive patients. Results There was no statistical difference between the positive rates of LHBs and HBV DNA in L group, the result to were 95.21% and 86.17% respectively (P>0.05), and showed a good correlation. The positive rates of HBV DNA and LHBs among S and DN groups were 73.67%, 81.48% and 42.32%, 55.56% respectively and showed statistical difference between LHBs and HBV DNA(P<0.01). In 346 cases with HBeAg-negative, the positive rates of LHBs and HBV DNA were 71.39% and 43.35% respectively and showed statistical difference (P<0.01). Among 196 HBV DNA negtive cases, the positive rates of LHBs were 68.88 %. Conclusions LHRs is correlated closely with the levels of HBV DNA, it would be a good immuno-serological index reflecting HBV replication and prognosis criterion for HBeAg negative patients.
