CAJ | 학술논문

目的研究苦参碱对人喉癌细胞Hep-2的生长抑制作用及凋亡机制.结果四甲基噻唑蓝(MTT)法测定苦参碱对细胞的抑制作用,荧光显微镜、DNA琼脂糖凝胶电泳、流式细胞仪技术(FCM)观察细胞周期及凋亡.结果不同浓度苦参碱对Hep-2细胞有明显抑制作用,且呈时间依赖性及剂量依赖性;FCM检测结果显示:S期细胞比例及凋亡率增高,G2/M期细胞比例下降,且呈剂量依赖性;形态学发现Hep-2细胞染色质凝聚,核碎裂,凋亡小体形成;DNA琼脂糖凝胶电泳显示典型的梯形条带.结论苦参碱可能是通过将人喉癌细胞周期阻滞在S期,诱导喉癌细胞凋亡,抑制其增殖,发挥其抗癌功能.
목적 연구고삼감대인후암세포Hep-2적생장억제작용급조망궤제.결과 사갑기새서람(MTT)법측정고삼감대세포적억제작용,형광현미경、DNA경지당응효전영、류식세포의기술(FCM)관찰세포주기급조망.결과 불동농도고삼감대Hep-2세포유명현억제작용,차정시간의뢰성급제량의뢰성;FCM검측결과 현시:S기세포비례급조망솔증고,G2/M기세포비례하강,차정제량의뢰성;형태학발현Hep-2세포염색질응취,핵쇄렬,조망소체형성;DNA경지당응효전영현시전형적제형조대.결론 고삼감가능시통과장인후암세포주기조체재S기,유도후암세포조망,억제기증식,발휘기항암공능.
Objective To investigate the mechanism of grow-inhibition and apoptosis induced by marine on Hep-2 line. Methods The growth inhibiting rate of matrine on Hep-2 cells was detected by MTT assay. Fluorescence microscope, DNA gel electrophoresis and flow cytometry (FCM) were applied to determine the presence of apoptosis and cell cycle. Results Matrine had cytotoxic effect to Hep-2 cells in a time- and dose-dependent manner. The FCM analysis showed that the number of Hep-2 cells of S phase and the apoptosis rate increased, while the number of G2/M phase decreased. There were morphological changes including chromatin condensation, nuclear fragmentation and apeptotic bodies formed. DNA gel electrophoresis analysis showed typical DNA ladder of apoptosis. Conclusion Matrine inhibits cell prolif-eration by blocking Hep-2 cell cycle to S phase, and exerts its anti-carcinoma function by inducing apoptosis.